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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 9,930 results for: bli

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Showing: 7101 - 7120 of 9930 results

  1. pCMV-T7-SpCas9-BPNLS(SV40)-3xFLAG-P2A-mScarlet (MNW001)

    Plasmid
    #174091
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9 with a C-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized SpCas9 with BPNLS-3xFLAG-P2A-mScarlet
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-mScarlet
    Expression
    Mammalian
    Promoter
    pCMV and T7
    Available Since
    Sept. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    174091-plasmid-map-sequence-id-341917

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  3. PTPN11

    Plasmid
    #38965
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PTPN11 (PTPN11 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  4. HSD17B4

    Plasmid
    #39156
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    HSD17B4 (HSD17B4 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  6. pAAV2-CAGIG-LAMP1-mGL

    Plasmid
    #222394
    Purpose
    To express in mammalian cells, the lysosome-associated membrane glycoprotein 1 (LAMP1) fused with the FP protein mGreenLantern. This plasmid used to label lysosomes in transfected and infected cells.
    Depositor
    Pantelis Tsoulfas
    Insert
    LAMP1 and mGreenlantern (LAMP1 Synthetic, Human, Homo Sapiens)
    Use
    AAV
    Tags
    LAMP1 and mGreenLantern
    Expression
    Mammalian
    Promoter
    CAG or CAGIG
    Available Since
    Sept. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    222394-plasmid-map-sequence-id-446519

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  7. ACE2 g3

    Plasmid
    #153013
    Purpose
    A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing mCherry
    Depositor
    Jason Sheltzer
    Insert
    ACE2 (ACE2 Human)
    Use
    CRISPR and Lentiviral
    Tags
    mCherry
    Available Since
    June 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    153013-plasmid-map-sequence-id-298679

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  8. Hs.KRAS4b G13C

    Plasmid
    #83145
    Purpose
    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), G13C mutation
    Depositor
    Dominic Esposito
    Insert
    KRAS (KRAS Human)
    Use
    Gateway Cloning
    Mutation
    G13C
    Available Since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    83145-plasmid-map-sequence-id-158108

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  11. CD44I-c013

    Plasmid
    #175450
    Purpose
    Protein expression/secretion in mammalian cells. CD44 Link module (exons 2-5), A20-P220.
    Depositor
    Opher Gileadi
    Insert
    CD44 (CD44 Human)
    Tags
    His6
    Expression
    Mammalian
    Available Since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175450-plasmid-map-sequence-id-353226

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  12. Hs.KRAS4b Q61R

    Plasmid
    #83135
    Purpose
    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), Q61R mutation
    Depositor
    Dominic Esposito
    Insert
    KRAS (KRAS Human)
    Use
    Gateway Cloning
    Mutation
    Q61R
    Available Since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    83135-plasmid-map-sequence-id-158098

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  13. Hs.KRAS4a G12C

    Plasmid
    #83167
    Purpose
    Gateway ORF Entry clone of human KRAS4A [NM_033360.3] with stop codon (for native or N-terminal fusions), G12C mutation
    Depositor
    Dominic Esposito
    Insert
    KRAS (KRAS Human)
    Use
    Gateway Cloning
    Mutation
    G12C
    Available Since
    Sept. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    83167-plasmid-map-sequence-id-158130

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  14. tet-pLKO.puro_shGAC

    Plasmid
    #110423
    Purpose
    shGAC, silence glutaminase GAC isoform, doxycycline inducible, puromycin selection
    Depositor
    Sandra Martha Gomes Dias
    Insert
    GLS glutaminase (GLS Human)
    Use
    Lentiviral and RNAi
    Expression
    Mammalian
    Promoter
    H1/TO (RNA PolIII)
    Available Since
    June 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    110423-plasmid-map-sequence-id-213314

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  15. MECR

    Plasmid
    #38850
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    MECR (Mecr Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  18. HSP90B1

    Plasmid
    #39076
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    HSP90B1 (HSP90B1 Human)
    Tags
    TEV-His6-Flag
    Expression
    Bacterial
    Available Since
    May 7, 2013
    Availability
    Academic Institutions and Nonprofits only

  19. pALS3-Ma-SUMO-sfGFP-WT

    Plasmid
    #212122
    Purpose
    Fluorescence control plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses SUMO-sfGFP-WT and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.
    Depositor
    Ryan Mehl
    Inserts
    SUMO-sfGFP-WT
    M. alvus Pyl-tRNA(6)
    Tags
    His6 and SUMO
    Expression
    Bacterial
    Promoter
    araC and lpp
    Available Since
    Sept. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    212122-plasmid-map-sequence-id-444187

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  20. Lamin A (R527P)-mRFP

    Plasmid
    #124272
    Purpose
    Lamin A mutant (R527P), C-terminally tagged with mRFP. Mutation associated with EDMD myopathy.
    Depositor
    Eric Schirmer
    Insert
    LMNA (R527P) (LMNA Human)
    Tags
    mRFP
    Expression
    Mammalian
    Mutation
    LMNA (R527P)
    Available Since
    May 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    124272-plasmid-map-sequence-id-241977

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Showing: 7101 - 7120 of 9930 results