We narrowed to 23,678 results for: crispr

1. pFETCh_ZFP1_iso1

   Plasmid

   #86294

   Purpose

   Donor vector for 3' FLAG tag of human ZFP1_iso1

   Depositor

   Eric Mendenhall, Richard M. Myers

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   ZFP1_iso1 homology arms (ZFP1 Human)
   

   Use

   CRISPR

   Tags

   3XFLAG-P2A-NeoR

   Available Since

   Feb. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFETCh_ZSCAN9

   Plasmid

   #86268

   Purpose

   Donor vector for 3' FLAG tag of human ZSCAN9

   Depositor

   Eric Mendenhall, Richard M. Myers

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   ZSCAN9 homology arms (ZNF193 Human)
   

   Use

   CRISPR

   Tags

   3XFLAG-P2A-NeoR

   Available Since

   Jan. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCas2B

   Plasmid

   #82373

   Purpose

   dCas9 expressed from aTc-inducible promoter in acsA, B RBS: TCGAGA

   Depositor

   Brian Pfleger

   Article

   Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

   Insert

   dCas9
   

   Promoter

   PEZ3

   Available Since

   Oct. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCas8

   Plasmid

   #82380

   Purpose

   dCas9 without a promoter in acsA

   Depositor

   Brian Pfleger

   Article

   Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

   Insert

   dCas9
   

   Promoter

   none

   Available Since

   Sept. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCas7

   Plasmid

   #82379

   Purpose

   dCas9 expressed from low constituve promoter, c225, in acsA

   Depositor

   Brian Pfleger

   Article

   Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

   Insert

   dCas9
   

   Promoter

   c225

   Available Since

   Sept. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCas2E

   Plasmid

   #82376

   Purpose

   dCas9 expressed from aTc-inducible promoter in acsA, E RBS: AGGGCG

   Depositor

   Brian Pfleger

   Article

   Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

   Insert

   dCas9
   

   Promoter

   PEZ3

   Available Since

   Sept. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCas2A

   Plasmid

   #82372

   Purpose

   dCas9 expressed from aTc-inducible promoter in acsA, A RBS: AGGAGA

   Depositor

   Brian Pfleger

   Article

   Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

   Insert

   dCas9
   

   Promoter

   PEZ3

   Available Since

   Sept. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCas28

   Plasmid

   #82385

   Purpose

   sgRNA targeting YFP expressed from pJ23108 in the NS1 locus with kanamycin resistance

   Depositor

   Brian Pfleger

   Article

   Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

   Insert

   gRNA
   

   Promoter

   PJ23108

   Available Since

   Sept. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCas27

   Plasmid

   #82384

   Purpose

   sgRNA targeting YFP expressed from pJ23117 in the NS1 locus with kanamycin resistance

   Depositor

   Brian Pfleger

   Article

   Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

   Insert

   gRNA
   

   Promoter

   PJ23117

   Available Since

   Sept. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. MsMUT_sgRNA1

    Plasmid

    #111296

    Purpose

    CRISPR KO murine MUT

    Depositor

    Vamsi Mootha

    Article

    Shen et al Cell. 2017 Nov 2;171(4):771-782.e11. doi

    Insert

    sgRNA1 against murine MUT
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Availability

    Academic Institutions and Nonprofits only

    

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11. MsMUT_sgRNA3

    Plasmid

    #111297

    Purpose

    CRISPR KO murine MUT

    Depositor

    Vamsi Mootha

    Article

    Shen et al Cell. 2017 Nov 2;171(4):771-782.e11. doi

    Insert

    sgRNA3 against murine MUT
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJK412

    Plasmid

    #155371

    Purpose

    dCas9 oscillator v1 (dCRv1) + [dCas9 + PA4-mVenus]

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas9 (bacteria)
    PA4-mVenus
    dCas9 sgRNA oscillator v1
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    D10A H840A (catalytically inactive)

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHR-EF1a-dCas9-HA-BFP-KRAB-NLS

    Plasmid

    #102244

    Purpose

    Lentiviral expression of dCas9-HA-BFP-KRAB-NLS for CRISPRi driven by a hEF1alpha promoter

    Depositor

    Jacob Corn

    Insert

    dCas9-HA-BFP-KRAB-NLS
    

    Use

    CRISPR and Lentiviral

    Tags

    HA-TagBFP-KRAB-NLS

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEF1a-PEmax-P2A-GFP

    Plasmid

    #202000

    Purpose

    Based on the pCMV-PEmax-P2A-GFP, we replaced the CMP promoter with the EF1a promoter for expression in iPSC.

    Depositor

    Daniel Geschwind

    Insert

    PEmax-P2A-GFP
    

    Use

    CRISPR and Synthetic Biology

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. EFS-FLAG-SpCas9-6xNLS(c-Myc)-P2A-Puro (pRG214)

    Plasmid

    #221385

    Purpose

    Lentiviral expression of SpCas9-6xNLS(c-Myc) with puromycin resistance gene

    Depositor

    Junwei Shi

    Insert

    SpCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    June 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. PX408 Francisella tularensis subsp. novicida Cas9

    Plasmid

    #68705

    Purpose

    Francisella tularensis subsp. novicida Cas9

    Depositor

    Feng Zhang

    Insert

    FtCas9
    

    Use

    CRISPR

    Tags

    HA-NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. miniTurbo-dCasRx

    Plasmid

    #219821

    Purpose

    EF1α-driven expression of miniTurbo fused to dCasRx with GFP (Adapted from plasmid #153209)

    Depositor

    Philipp Maass

    Insert

    dCasRx
    

    Use

    CRISPR

    Tags

    miniTurbo

    Expression

    Mammalian

    Promoter

    EF1a and hU6

    Available Since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. p-dCas9-dTET1-H1672Y-D1674A-Hygro

    Plasmid

    #104413

    Purpose

    transient expression of dCas9-dTET1 fusion protein

    Depositor

    Stephan Beck

    Insert

    dTET1
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    H1672Y-D1674A

    Promoter

    CMV promoter

    Available Since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. PX400 Campylobacter lari CF89-12 Cas9

    Plasmid

    #68336

    Purpose

    Campylobacter lari CF89-12 Cas9

    Depositor

    Feng Zhang

    Insert

    ClCas9
    

    Use

    CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCB32

    Plasmid

    #111911

    Purpose

    Expresses gRNA to target Nte1 locus and has HygR marker

    Depositor

    Yi Tang

    Insert

    gRNA cassette targeting Nte1 locus
    

    Use

    CRISPR

    Expression

    Bacterial and Yeast

    Promoter

    SNR52p

    Available Since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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