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pRSETB/mStayGold

Plasmid

#212017

Purpose

mStayGold has an n1 adaptor at its N-terminus and is useful for C-terminal tagging. This construct is used also for expression of mStayGold itself.

Depositor

Atsushi Miyawaki

Article

Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Insert

mStayGold

Expression

Bacterial

Promoter

T7

Available Since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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TB:4xHRE_RE:MiniP

Plasmid

#178313

Purpose

4 copies of the HRE DNA binding motif

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Methods Mol Biol. 2022;2524:409-432. doi

Insert

4 copies of the heat shock DNA binding motif

Use

Luciferase and Synthetic Biology

Expression

Mammalian

Available Since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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pDD428

Plasmid

#202081

Purpose

Cas9/sgRNA plasmid targeting the C-terminus of Myh9

Depositor

Daniel Dickinson

Article

Shi et al Development. 2023 May 15;150(10):dev2013

Insert

pEF1a-mCherry-Cas9 + Myh9 CT sgRNA (Myh9 S. pyogenes (Cas9))

Use

CRISPR

Promoter

EF1a

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-Rab7Q67L

Plasmid

#169038

Purpose

Express human constitutively active form of Rab7a(Q67L) in mammalian cells

Depositor

Gia Voeltz

Article

Wu et al Dev Cell. 2021 Jan 11;56(1):52-66.e7. do

Insert

Rab7a (RAB7A Human)

Tags

acGFP1

Expression

Mammalian

Mutation

Q67L

Promoter

CMV

Available Since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pQCXIP-HA.Tetherin

Plasmid

#41069

Depositor

Paul Spearman

Article

Hammonds et al PLoS Pathog. 2010 Feb 5;6(2):e1000749.

Insert

Tetherin (BST2 Human)

Use

Retroviral

Tags

HA and IRES Puro

Expression

Mammalian

Promoter

CMV/MSV

Available Since

Nov. 5, 2012

Availability

Academic Institutions and Nonprofits only
Human ICAM-1

Plasmid

#8632

Depositor

Timothy Springer

Article

Staunton et al Cell 1988 Mar 25;52(6):925-33.

Insert

ICAM-1 (ICAM1 Human)

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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pWM_12x601_25bpLinker

Plasmid

#157788

Purpose

EcoRV digestion produces 12x601 chromatin assembly construct with 25 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

12x601_25bp linker

Expression

Bacterial

Available Since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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qTAG-C-mStayGold-EFS-Puro

Plasmid

#227256

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mStayGold-EFS-Puro cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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HA GSK3 beta wt pcDNA3

Plasmid

#14753

Purpose

Mammalian expression of human GSK3 beta with HA tag

Depositor

Jim Woodgett

Article

He et al Nature. 1995 Apr 13. 374(6523):617-22.

Insert

GSK3 beta (GSK3B Human)

Tags

HA

Expression

Mammalian

Available Since

April 12, 2007

Availability

Industry, Academic Institutions, and Nonprofits

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pET-15b-EK_C122S_His5

Plasmid

#49048

Purpose

Expresses the pentahistidine-tagged catalytic domain of bovine enteropeptidase in E. coli

Depositor

Hans Brandstetter

Article

Skala et al J Biotechnol. 2013 Dec;168(4):421-5. doi

Insert

Enteropeptidase, peptidase domain (TMPRSS15 Bovine)

Tags

5xHis

Expression

Bacterial

Mutation

P92R, E185D, C122S

Promoter

T7

Available Since

Jan. 14, 2014

Availability

Academic Institutions and Nonprofits only

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pX330-AAVS1

Plasmid

#227272

Purpose

Expresses SpCas9 and a sgRNA targeting the AAVS1 loci for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting AAVS1 (AAVS1 Synthetic)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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HA GSK3 beta K85A pcDNA3

Plasmid

#14755

Depositor

Jim Woodgett

Article

Hughes et al EMBO J. 1993 Feb . 12(2):803-8.

Insert

GSK3 beta K85A (GSK3B Human)

Tags

HA

Expression

Mammalian

Mutation

K85A. Kinase dead mutant.

Available Since

April 12, 2007

Availability

Industry, Academic Institutions, and Nonprofits

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pWM_12x601_15bpLinker

Plasmid

#157786

Purpose

EcoRV digestion produces 12x601 chromatin assembly construct with 15 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

12x601_15bp linker

Expression

Bacterial

Available Since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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pCMVHA SUZ12

Plasmid

#24232

Purpose

Mammalian expression of human SUZ12 with HA tag

Depositor

Kristian Helin

Article

Pasini et al EMBO J. 2004 Oct 13. 23(20):4061-71.

Insert

SUZ12 (SUZ12 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only

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pLIB

Plasmid

#80610

Purpose

pLIB - Library vector (biGBac system for expression of protein complexes in insect cells)

Depositor

Jan-Michael Peters

Article

Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

Type

Empty backbone

Expression

Insect

Promoter

polyhedrin

Available Since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pKH144_mCherry-YIPF4 WT

Plasmid

#214972

Purpose

mCherry tagged YIPF4

Depositor

Wade Harper

Article

Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

Insert

mCherry-YIPF4 (YIPF4 Human)

Use

Lentiviral

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-HuR

Plasmid

#121162

Purpose

Mammalian expression of GFP tagged HuR

Depositor

Christine Mayr

Article

Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

Insert

HuR (ELAVL1 )

Expression

Mammalian

Available Since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pLentiCMV-Blast

Plasmid

#125133

Purpose

Lentiviral empty backbone control vector with CMV promoter and Blasticidin resistance gene

Depositor

Jin Chen

Article

Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

Type

Empty backbone

Use

Lentiviral

Available Since

May 24, 2021

Availability

Academic Institutions and Nonprofits only

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pFGAMS-mCherry

Plasmid

#166024

Purpose

Expresses human PFAS-mCherry fusion in mammalian cells

Depositor

Stephen Benkovic

Article

Doigneaux et al J Biol Chem. 2020 Jul 10;295(28):9551-95

Insert

PFAS-mCherry (PFAS Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pDEST14-Avitag-SpyCatcher002

Plasmid

#102828

Purpose

Bacterial expression of SpyCatcher002 with a peptide tag enabling site-specific biotinylation

Depositor

Mark Howarth

Article

Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

Insert

Avitag-SpyCatcher002

Tags

Avitag, GSSGS, His6, and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

Dec. 20, 2017

Availability

Academic Institutions and Nonprofits only

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