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343 pTol2-fli1epDest

Plasmid

#73486

Purpose

Tol2 vector with chimeric endothelial enhancer/promoter upstream of attR1/R2 cassette and late SV40 pA signal

Depositor

Nathan Lawson

Publication

Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

Insert

fli1ep

Available since

March 7, 2016

Availability

Academic Institutions and Nonprofits only

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pBCV

Plasmid

#62659

Purpose

Mammalian expression of FLPo recombinase from the broadly active CAG promoter/enhancer

Depositor

Masato Ohtsuka

Publication

Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

Insert

FLPo

Expression

Mammalian

Available since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

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pICH47811

Plasmid

#48008

Depositor

Sylvestre Marillonnet

Publication

Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

Type

Empty backbone

Use

Unspecified

Available since

Dec. 3, 2013

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

5 Articles

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pSLQ10871

Plasmid

#183962

Purpose

U6-SKSKSO(crRNA array)-CAG-dCas12a(WT)-miniVPR

Depositor

Stanley Qi

Publication

Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

dCas12a(wildtype)
poly-crRNA array targeting Sox2-Klf4-Oct4

Use

CRISPR

Tags

HA

Expression

Mammalian

Mutation

D832A

Promoter

CAG and U6

Available since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pMD19T-psba1-Ppsba2-dCas9-SpR

Plasmid

#73220

Purpose

Contains dCas9 from S. pyogenes under constitutive promoter. Suicide vector inserts into psba1 site of Synechocystis. Carries spectinomycin resist. Recommend E. coli Copy cutter for propogation.

Depositor

Paul Hudson

Publication

Yao et al ACS Synth Biol. 2015 Dec 28.

Insert

dCas9 from S. pyogenes

Tags

c-myc

Expression

Bacterial

Mutation

Silent mutation at bp 1341 A->C to remove an E…

Promoter

PpsbA2

Available since

March 15, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pHR_TRE3GS-->sGFP-P2A-BFP

Plasmid

#162234

Purpose

Lentiviral vector for the expression of TRE3GS-->sGFP-P2A-BFP in mammalian cells

Depositor

Wendell Lim

Publication

Toda et al Science. 2020 Oct 16;370(6514):327-331.

Insert

EGFP and BFP

Use

Lentiviral

Expression

Mammalian

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pUltra-FPheKRS

Plasmid

#136631

Purpose

Genetic Incorporation 4-fluorophenyl carbamate lysine (FPheK) into proteins

Depositor

Han Xiao

Publication

Yu et al Bioconjug Chem. 2018 Nov 21;29(11):3522-

Insert

FPheKRS

Expression

Bacterial

Promoter

Tac1 promoter

Available since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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Seattle_IPDA_control_9_003

Plasmid

#167349

Purpose

ddPCR gating control for droplets containing either gag+env targets OR tat + env targets

Depositor

Florian Hladik

Publication

Levy et al Cell Rep Med. 2021 Apr 12;2(4):100243. d

Insert

gag_tat_env

Use

Other

Available since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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Seattle_IPDA_control_6_002

Plasmid

#167348

Purpose

ddPCR gating control for droplets containing either gag+5'pol targets OR 3'pol+tat targets

Depositor

Florian Hladik

Publication

Levy et al Cell Rep Med. 2021 Apr 12;2(4):100243. d

Insert

pol_gag_tat

Use

Other

Available since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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Seattle_IPDA_control_29_006

Plasmid

#167352

Purpose

ddPCR gating control for droplets containing either env+5'pol targets OR env+3'pol targets

Depositor

Florian Hladik

Publication

Levy et al Cell Rep Med. 2021 Apr 12;2(4):100243. d

Insert

pol_env

Use

Other

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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Seattle_IPDA_control_14_004

Plasmid

#167350

Purpose

ddPCR gating control for droplets containing the env target

Depositor

Florian Hladik

Publication

Levy et al Cell Rep Med. 2021 Apr 12;2(4):100243. d

Insert

env

Use

Other

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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Seattle_IPDA_control_32_007

Plasmid

#167353

Purpose

ddPCR gating control for droplets containing 5'pol or 3'pol targets

Depositor

Florian Hladik

Publication

Levy et al Cell Rep Med. 2021 Apr 12;2(4):100243. d

Insert

pol

Use

Other

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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Seattle_IPDA_control_17_005

Plasmid

#167351

Purpose

ddPCR gating control for droplets containing gat and tat targets

Depositor

Florian Hladik

Publication

Levy et al Cell Rep Med. 2021 Apr 12;2(4):100243. d

Insert

gat_tat

Use

Other

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pDGE504

Plasmid

#153277

Purpose

Intermediate sgRNA array cloning vector position 2

Depositor

Johannes Stuttmann

Publication

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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psgRNA-ccdB

Plasmid

#99915

Purpose

Basic cloning vector for the expression of imaging-based optimization sgRNA

Depositor

Yujie Sun

Publication

Shao et al ACS Synth Biol. 2017 Sep 7. doi: 10.1021

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available since

Oct. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pM

Plasmid

#64179

Purpose

harbours marker gene MET17

Depositor

Markus Ralser

Publication

Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

Type

Empty backbone

Expression

Bacterial and Yeast

Available since

Aug. 24, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBDR

Plasmid

#62663

Purpose

Donor vector for tdTomato knock-in via landing pad

Depositor

Masato Ohtsuka

Publication

Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

Insert

tdTomato

Use

Cre/Lox

Available since

Aug. 10, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMOS017E: Entry vector for: ratiometric pHluorin (cytosolic)

Plasmid

#163069

Purpose

Entry vector for: ratiometric pHluorin (cytosolic)

Depositor

Adam Cohen

Publication

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ratiometric pHluorin

Use

Gateway Cloning

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pSLQ8518 PB-PGK-N term (1-406) dLbCpf1-EE12RR345L-pA

Plasmid

#140221

Purpose

Constitutive PGK driven expression of N terminal dCpf1 (Cas12a) 406-split half with leucine zipper

Depositor

Stanley Qi

Publication

Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

dLbCpf1 (Cas12a) N terminal half (406) with zipper

Expression

Mammalian

Promoter

PGK

Available since

April 21, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-FDIO-SYP-HRP

Plasmid

#117187

Purpose

Plasmid name in publication: pAAV-FDIO-SV-HRP. Peroxidase reporter for multiplexed electron microscopy labeling

Depositor

David Ginty

Publication

Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

Insert

SYP-HRP

Use

AAV; Flp/frt

Expression

Mammalian

Promoter

EF1a

Available since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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