We narrowed to 18,457 results for: multi

1. pEY68

   Plasmid

   #191065

   Purpose

   ceh-14 fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

   Depositor

   Oliver Hobert, Eviatar Yemini

   Article

   Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

   Insert

   ceh-14 (ceh-14 Nematode)
   

   Expression

   Worm

   Mutation

   None

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. FRB-CFP-ras

   Plasmid

   #87451

   Purpose

   rapamycin binding motif of mTOR (FRB) anchored to the plasma membrane

   Depositor

   Xiaodong Liu

   Article

   Liu et al Elife. 2017 Jan 6;6. pii: e21989. doi: 1

   Insert

   FRB (MTOR Human)
   

   Tags

   CFP and ras fragment

   Expression

   Mammalian

   Mutation

   FKBP and rapamycin binding domain only

   Promoter

   CMV

   Available Since

   March 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBCC155-MXMT

   Plasmid

   #79816

   Purpose

   CFP-based BiFC vector for plants (Positive control: MXMT)

   Depositor

   Yutaka Kodama, Masamitsu Wada

   Article

   Kodama et al Plant Mol Biol. 2009 May;70(1-2):211-7.

   Insert

   CaMXMT1
   

   Use

   Reporter plasmid

   Tags

   CC155/CFP (155–239)

   Expression

   Plant

   Promoter

   CaMV 35S promoter and CaMV35S

   Available Since

   Dec. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBS U6 WIP RNAi

   Plasmid

   #11774

   Depositor

   Jack Strominger

   Article

   Krzewski et al J Cell Biol. 2006 Apr 10. 173(1):121-32.

   Insert

   WIP RNAi (WIPF1 Human)
   

   Use

   RNAi

   Expression

   Mammalian

   Available Since

   Feb. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

5. pJR2953

   Plasmid

   #122549

   Purpose

   Expression of a C. elegan codon optimized florescent protein (CemCardinal2) under the intestinal specific promoter nhx-2

   Depositor

   Gary Silverman

   Article

   Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do

   Insert

   Pnhx-2_CemCardinal2
   

   Expression

   Bacterial

   Promoter

   Pnhx-2

   Available Since

   March 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. NLS-dCas9-HA-NLS-GFP4-VPR

   Plasmid

   #183924

   Purpose

   VPR activation domain coupled to catalytically inactive dCas9 for localization; contains a GFP tetramer fpr labeling and as a spacer

   Depositor

   Karsten Rippe

   Article

   Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

   Insert

   dCas9-GFP4-VPR
   

   Use

   CRISPR

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available Since

   July 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMIII-Neo-E2Crimson

   Plasmid

   #153425

   Purpose

   Retroviral (MSCV) expression of E2-Crimson fluorescent protein

   Depositor

   Maki Nakayama

   Article

   Mann et al Front Immunol. 2020 Apr 9;11:633. doi: 1

   Insert

   E2-Crimson
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Aug. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDGE663

   Plasmid

   #153261

   Purpose

   M6E shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

   Depositor

   Johannes Stuttmann

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDGE665

   Plasmid

   #153263

   Purpose

   M8E shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

   Depositor

   Johannes Stuttmann

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDGE654

    Plasmid

    #153252

    Purpose

    M1E shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEY64

    Plasmid

    #191061

    Purpose

    aqp-6 fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

    Depositor

    Oliver Hobert, Eviatar Yemini

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    aqp-6 (aqp-6 Nematode)
    

    Expression

    Worm

    Mutation

    None

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. LentiU6-mHbb-bh1_ gRNA_1-MS2-Puro

    Plasmid

    #192681

    Purpose

    Lentiviral expression of sgRNA targeting mHbb-bh1promoter to activate mouse Hbb-bh1 transcription

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    Mouse Hbb-bh1 activating gRNA #1 (Hbb-bh1 Mouse)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSG5-HA-CaMKKbeta rat FL WT

    Plasmid

    #33321

    Depositor

    Anthony Means

    Article

    Green et al J Biol Chem. 2011 Aug 12;286(32):28066-7

    Insert

    Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Rat)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    S3V, G96R, and P108L

    Promoter

    SV40

    Available Since

    April 17, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pGL4.13/GUG-FFLuc-3XFLAG

    Plasmid

    #127334

    Purpose

    Express 3xFLAG tagged firefly luciferase (FFLuc) from GUG start codon

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    Firefly luciferase
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    SV40

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBGC155-MXMT

    Plasmid

    #79818

    Purpose

    GFP(S65T)-based BiFC vector for plants (Positive control: MXMT)

    Depositor

    Yutaka Kodama, Masamitsu Wada

    Article

    Kodama et al Plant Mol Biol. 2009 May;70(1-2):211-7.

    Insert

    CaMXMT1
    

    Use

    Reporter plasmid

    Tags

    GC155/GFP (155–239)

    Expression

    Plant

    Promoter

    CaMV 35S promoter and CaMV35S

    Available Since

    Dec. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1(+)MBII-85-5'/3'ss mut

    Plasmid

    #67645

    Purpose

    MBII85 snoRNA expression cassett, contains snoRNA in natural intron sorounded by natural exons. To improve snoRNA expression efficiency 5' and 3' splice sites were mutated to consensus sequences.

    Depositor

    Stefan Stamm

    Article

    Falaleeva et al Gene. 2015 Jul 26. pii: S0378-1119(15)00

    Insert

    MBII85 snoRNA
    

    Expression

    Mammalian

    Mutation

    5' and 3' splice sites mutations

    Available Since

    Aug. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. p3xFlag-CMV14-hISD11

    Plasmid

    #31897

    Depositor

    Gino Cortopassi

    Article

    Shan et al Hum Mol Genet. 2007 Apr 15;16(8):929-41.

    Insert

    ISD11 (LYRM4 Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

18. KTK_001

    Plasmid

    #180517

    Purpose

    Empty backbone for creation of entry parts for the KTK, contains sfGFP dropout

    Depositor

    Tom Ellis

    Article

    Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    April 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pR6Kg-Alpha1spm

    Plasmid

    #165866

    Purpose

    Modified Alpha level R6K_ origin GB Cloning vector, Conditionally replicative ori, requires pir gene expression requires pir gene expression requires EC100D or EC100D116 bacterial strain (DH10B derived) uses blue-white screening

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBP-pET-RBS

    Plasmid

    #72981

    Purpose

    PET RBS - derived from pET vectors (5' GTAC/3' CATA fusion)

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    pET RBS derived from pET vectors
    

    Use

    Synthetic Biology

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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