We narrowed to 22,830 results for: Sis

1. hMEF2A WT

   Plasmid

   #118354

   Purpose

   This plasmid expresses human MEF2A-1 WT.

   Depositor

   Lea Sistonen

   Article

   Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

   Insert

   hMEF2A (MEF2A Human)
   

   Use

   Tags

   myc and 6xHIS

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available Since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pS648∙CsR

   Plasmid

   #197860

   Purpose

   Expresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteria

   Depositor

   Susanne Häußler

   Article

   Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

   Insert

   gRNA scaffold - Gm resistance cassette - incP oriT
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   PEM7

   Available Since

   Oct. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. gRNA NIA TLS1

   Plasmid

   #196980

   Purpose

   Expresses two gRNAs targeting Arabidopsis NIA1 gene for knockout. Each gRNA is fused to TLS1 mobility signal that confers graft mobility.

   Depositor

   Friedrich Kragler

   Article

   Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   AtNIA1 gRNAs fused to TLS1 tags
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   pU6-26, pU6-29

   Available Since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-SF3B1-K700E-reporter

   Plasmid

   #200318

   Purpose

   A fluorescent reporter for SF3B1 K700E mutation. Co-expresses a blasticidin resistance gene for selection. Includes LoxP sites for Cre-mediated deletion of reporter cassette.

   Depositor

   Lili Wang

   Article

   Fernandez et al Cancer Res Commun. 2024 Aug 28. doi: 10.

   Insert

   BSD-2A-EGFPi
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-a

   Available Since

   Aug. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pIRES-Puro-MGMT

   Plasmid

   #187654

   Purpose

   Expresses hMGMT together with the puromycin resistance gene via an IRES.

   Depositor

   Robert Sobol

   Article

   Li et al Cancers (Basel). 2022 Jul 22;14(15). pii

   Insert

   MGMT (MGMT Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHEE901-A3A

   Plasmid

   #229044

   Purpose

   nCas9 mediated-base editing with NGG PAM recognition motif in Arabidopsis. A3A-nCas9-UGI includes D10A in Cas9.

   Depositor

   Huawei Zhang

   Article

   Wang et al Genome Biol. 2024 Aug 9;25(1):215. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMax_mOrange_G299C

   Plasmid

   #177829

   Purpose

   Site-specific mutagenesis of mOrange for the purposes of creating a site-specific G:G mispair for the purposes of measuring MMR activity via Host Cell Reactivation (HCR).

   Depositor

   Leona Samson, Zachary Nagel

   Article

   Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

   Insert

   mOrange_G299C
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   G299C

   Promoter

   CMV

   Available Since

   Nov. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA-hHSF1 WT

   Plasmid

   #71724

   Purpose

   plasmid expressing myc-his tag hHSF1 WT

   Depositor

   Lea Sistonen

   Article

   Budzynski et al Mol Cell Biol. 2015 Jul;35(14):2530-40.

   Insert

   HSF1 (HSF1 Human)
   

   Use

   Tags

   MYC 6xHIS

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBAD miniSOG

   Plasmid

   #91774

   Purpose

   Bacterial expression of a fluorescent protein, mini Singlet Oxygen Generator (miniSOG)

   Depositor

   Erik Rodriguez, Roger Tsien

   Article

   Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

   Insert

   mini Singlet Oxygen Generator
   

   Use

   Tags

   Hexa-Histidine tag

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD

   Available Since

   June 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRelA*

    Plasmid

    #175590

    Purpose

    Tet inducible, KAN Resistant, fluor labelled (YFP) synthesis domain of the E. coli relA gene on a low copy backbone. Used to induce controllable synthesis of ppGpp in E. coli.

    Depositor

    Greg Bokinsky

    Article

    Buke et al Curr Biol. 2022 Feb 28;32(4):870-877.e5.

    Insert

    Catalytic domain of E.coli relA gene.
    

    Use

    Tags

    Fused with a flexible GS linker to mVenus

    Expression

    Bacterial

    Mutation

    Only the catalytic domain of the enzyme is used (…

    Promoter

    Available Since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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