We narrowed to 21,681 results for: mpl

1. pD649-HAsp-ROBO4-Fc(DAPA)-AviTag-6xHis

   Plasmid

   #156864

   Purpose

   Mammalian expression of secreted protein fused to Fc(DAPA)-Avi-6xHis.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   ROBO4 (ROBO4 Human)
   

   Use

   Tags

   Fc(DAPA)-AviTag-6xHis

   Expression

   Mammalian

   Mutation

   Fc region of human IgG contains D265A and P329A m…

   Promoter

   CMV/SP6

   Available since

   Jan. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pD649-HAsp-ROBO2-COMP5AP-AviTag-9xHis

   Plasmid

   #157555

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   ROBO2 (ROBO2 Human)
   

   Use

   Tags

   COMP5AP-AviTag-9xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/SP6

   Available since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-N2-6MYC-hMID2

   Plasmid

   #51035

   Purpose

   mammalian expression of myc-tagged human MID2

   Depositor

   Timothy Cox

   Article

   Short et al BMC Cell Biol. 2002;3:1. Epub 2002 Jan 4

   Insert

   MID2 (MID2 Human)
   

   Use

   Tags

   MYC

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 19, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pU6-(BsaI)_CBh-Cas9-T2A-mCherry

   Plasmid

   #135012

   Purpose

   Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 linked to mCherry via a T2A peptide

   Depositor

   Yosef Shaul

   Article

   Reuven et al Biomolecules. 2019 Oct 8;9(10). pii: bio

   Insert

   humanized S. pyogenes Cas9
   

   Use

   Tags

   3x Flag, 3xFLAG (N terminal on insert), NLS, and …

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTT3-SP-6xHis-NTRK1(FL)-FLAG

   Plasmid

   #157620

   Purpose

   Mammalian cell surface expression of His- and FLAG-tagged full-length protein for binding and signaling assays

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   NTRK1 (NTRK1 Human)
   

   Use

   Tags

   6xHis and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRS-mEmerald-VIM

   Plasmid

   #62849

   Purpose

   Donor vector for gene editing at human VIM locus

   Depositor

   Gaudenz Danuser

   Article

   Gan et al Cell Syst. 2016 Sep 21. pii: S2405-4712(

   Insert

   mEmerald-VIM (VIM Synthetic, Human)
   

   Use

   Yeast-e.coli shuttle vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. MSCV-IRES-GFP ASXL1 (1-479) 3x FLAG

   Plasmid

   #81023

   Purpose

   Cancer-associated ASXL1 truncation mutant in retroviral vector

   Depositor

   Anjana Rao

   Article

   Balasubramani et al Nat Commun. 2015 Jun 22;6:7307. doi: 10.

   Insert

   ASXL1 (ASXL1 Human)
   

   Use

   Tags

   3X FLAG

   Expression

   Mammalian

   Mutation

   Contains ASXL1 amino acids 1-479

   Promoter

   5’LTR

   Available since

   Nov. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pD649-HAsp-SPACA6-COMP5AP-AviTag-9xHis

   Plasmid

   #157274

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   SPACA6 (SPACA6 Human)
   

   Use

   Tags

   COMP5AP-AviTag-9xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/SP6

   Available since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. PX458-sgTSC2

   Plasmid

   #128098

   Purpose

   CRISPR gRNA against human TSC2 with Cas9 from S. pyogenes and 2A-EGFP

   Depositor

   Chi Van Dang

   Article

   Walton et al Cell. 2018 Jun 28;174(1):72-87.e32. doi:

   Insert

   sgRNA against human TSC2 (TSC2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGAPTrap-mCherry-IRESMygro

    Plasmid

    #82505

    Purpose

    Vector targeting the mCherry gene to the GAPDH locus of human cells. mCherry is expressed from a 2A sequence at the C terminus of GAPDH. Hygromycin B resistance is encoded by the Mygro gene.

    Depositor

    Ed Stanley

    Article

    Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221

    Insert

    mCherry-IRESMygro
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. InsRA-EBFP2

    Plasmid

    #79797

    Purpose

    Insulin receptor isoform A with a inter-domain EBFP2 tag at extracellular region (between furin-like domain and transmembrane domain). For imaging insulin receptor trafficing.

    Depositor

    James Johnson

    Article

    Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:

    Insert

    InsRA (INSR Human)
    

    Use

    Tags

    EBFP2 extracellular

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 5, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. shBCL2L1 # 2

    Plasmid

    #42552

    Depositor

    William Hahn

    Article

    Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

    Insert

    shBCL2L1 (BCL2L1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2013

    Availability

    Academic Institutions and Nonprofits only

13. shBCL2L1 # 1

    Plasmid

    #42551

    Depositor

    William Hahn

    Article

    Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

    Insert

    shBCL2L1 (BCL2L1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pGAPTrap-eGFP-IRESMygro

    Plasmid

    #82503

    Purpose

    Vector targeting the eGFP gene to the GAPDH locus of human cells. eGFP is expressed from a 2A sequence at the C terminus of GAPDH. Hygromycin B resistance is encoded by the Mygro gene.

    Depositor

    Ed Stanley

    Article

    Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221

    Insert

    eGFP-IRESMygro
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGex-4T-2_GATE-16

    Plasmid

    #73518

    Purpose

    For recombinant expression of human GATE-16/GABARAPL2 in E. coli

    Depositor

    Dieter Willbold

    Article

    Ma et al J Biol Chem. 2013 Dec 27;288(52):37204-1

    Insert

    GATE-16 (GABARAPL2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

16. pD649-HAsp-THY1-COMP5AP-AviTag-9xHis

    Plasmid

    #157056

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    THY1 (THY1 Human)
    

    Use

    Tags

    COMP5AP-AviTag-9xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/SP6

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSNAP-CAPZ

    Plasmid

    #69948

    Purpose

    Simultaneous expression of a His- and SNAP-tagged β1 subunit and untagged α1 subunits of chicken CapZ

    Depositor

    Jeff Gelles

    Article

    Bombardier et al Nat Commun. 2015 Nov 13;6:8707. doi: 10.

    Inserts

    CapZ beta 1 (CAPZB Chicken)
    CapZ alpha 1 (CAPZA1 Chicken)
    

    Use

    Tags

    His 6 and SNAP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBiex-1 AC30_eGFP

    Plasmid

    #82715

    Purpose

    Expresses a GFP-tagged recombinant adenylyl cyclase.

    Depositor

    Sivaraj Sivaramakrishnan

    Article

    Sommese et al Protein Sci. 2016 Aug 18. doi: 10.1002/p

    Insert

    Adenylyl Cyclase V/II (ADCY2, ADCY5 Human)
    

    Use

    Tags

    FLAG purification tag and eGFP

    Expression

    Bacterial and Insect

    Mutation

    Promoter

    T7 Promoter

    Available since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pD649-HAsp-PILRA-COMP5AP-AviTag-9xHis

    Plasmid

    #157142

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    PILRA (PILRA Human)
    

    Use

    Tags

    COMP5AP-AviTag-9xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/SP6

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEBTet-SNAP-CPAP

    Plasmid

    #136815

    Purpose

    Mammalian expression of the centrosomal protein CENPJ N-terminally fused to SNAP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    SNAP-CENPJ (CENPJ Synthetic, Human)
    

    Use

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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