We narrowed to 8,708 results for: sgRNA

1. pBTK614

   Plasmid

   #110608

   Purpose

   BTK assembled plasmid - Encodes dCas9 with no targeting sgRNA (used in Stage 2 assembly to repress target genes)

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

   Insert

   dCas9
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSLQ2827 pHR: U6-SpsgSV40 CMV-PYL1-KRAB-IRES-mCherry

   Plasmid

   #84260

   Purpose

   Expresses Sp sgSV40 gRNA with ABA-inducible KRAB and mCherry for diametric regulation

   Depositor

   Stanley Qi

   Article

   Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

   Inserts

   Sp sgSV40
   PYL1-KRAB
   

   Use

   CRISPR and Lentiviral

   Tags

   IRES-mCherry and PYL1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and mouse U6

   Available since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBTK601

   Plasmid

   #110607

   Purpose

   BTK assembled plasmid - encodes Cas9 with no targeting sgRNA (used with suicide plasmid for broad-host-range genome alteration)

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

   Insert

   Cas9
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMC-Z-SpCas9-gRNA-repeat-polyT-11

   Plasmid

   #197781

   Purpose

   Phytobrick (MoClo) Level 0 Part

   Depositor

   Daniel Voytas

   Article

   Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

   Insert

   gRNA repeat for U6 sgRNA assembly
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. gRNA-HEK3-Puro

   Plasmid

   #136282

   Purpose

   Expresses a guide RNA targeting HEK3 site

   Depositor

   Zhaohui Ye

   Article

   McGrath et al Nat Commun. 2019 Nov 25;10(1):5353. doi:

   Insert

   sgRNA-HEK3
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDECKO-mCherry MALAT1_Exon.1

   Plasmid

   #78540

   Purpose

   Experimental plasmid

   Depositor

   Roderic Guigo, Rory Johnson

   Article

   Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

   Insert

   gRNA1+scaffold+H1promoter+gRNA2
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and U6 (for expressing sgRNA)

   Available since

   July 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. Sha2Cas9

   Plasmid

   #192140

   Purpose

   Expresses Sha2Cas9, and cloning backbone for sgRNA

   Depositor

   Yongming Wang

   Article

   Wang et al PLoS Biol. 2022 Nov 30;20(11):e3001897.

   Insert

   Sha2Cas9
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. SpeCas9

   Plasmid

   #192139

   Purpose

   Expresses SpeCas9, and cloning backbone for sgRNA

   Depositor

   Yongming Wang

   Article

   Wang et al PLoS Biol. 2022 Nov 30;20(11):e3001897.

   Insert

   SpeCas9
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. Sa-SchCas9

   Plasmid

   #192137

   Purpose

   Expresses Sa-SchCas9, and cloning backbone for sgRNA

   Depositor

   Yongming Wang

   Article

   Wang et al Adv Sci (Weinh). 2022 Feb;9(4):e2104789.

   Insert

   Sa-SchCas9
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLG1-puro-sgULK1-1

    Plasmid

    #109004

    Purpose

    CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

    Depositor

    Jacob Corn

    Article

    Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

    Insert

    ULK1-sgRNA #1 (ULK1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6 promoter

    Available since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPTG-GG-sg2+sg3

    Plasmid

    #127199

    Purpose

    Plasmid expressing sgRNA2 (TACCACATTTGTAGAGGTT) & sgRNA3 (CAATGTATCTTATCATGTC) as polycistronic tRNA-gRNA from single U6 promoter

    Depositor

    Jordan Green

    Article

    Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

    Insert

    tRNA-gRNA
    

    Use

    Empty sgrna plasmid

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. sgp27#1

    Plasmid

    #102759

    Purpose

    Expresses sgRNA targeting mouse p27

    Depositor

    Jin Chen

    Article

    Song et al Oncogene. 2017 Jun 5. doi: 10.1038/onc.2

    Insert

    p27 (Cdkn1b Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJY-RpABE-FT_INF

    Plasmid

    #112874

    Purpose

    binary vector for INF (Induced Neo-Functionalization) of FT in Arabidopsis

    Depositor

    Jae-Young Yun, Jin-Soo Kim

    Article

    Kang et al Nat Plants. 2018 Jun 4. pii: 10.1038/s41

    Inserts

    RPS5A promoter
    ABE7.10
    U6-sgRNA targeting FT
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Sept. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pU6-(BsaI)_CBh-Cas9

    Plasmid

    #135010

    Purpose

    Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9

    Depositor

    Yosef Shaul

    Article

    Reuven et al Biomolecules. 2019 Oct 8;9(10). pii: bio

    Insert

    humanized S. pyogenes Cas9
    

    Use

    Tags

    3xFLAG (N terminal on insert)

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLG1-puro-sgATL2-1

    Plasmid

    #109008

    Purpose

    CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

    Depositor

    Jacob Corn

    Article

    Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

    Insert

    ATL2-sgRNA #1 (ATL2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6 promoter

    Available since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPN013

    Plasmid

    #91587

    Purpose

    Express sgRNA targeting human CSMD1

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    CSMD1 (CSMD1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDECKO-mCherry TFRC_B

    Plasmid

    #78544

    Purpose

    Experimental plasmid

    Depositor

    Roderic Guigo, Rory Johnson

    Article

    Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

    Insert

    gRNA1+scaffold+H1promoter+gRNA2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and U6 (for expressing sgRNA)

    Available since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. MTK234_037

    Plasmid

    #123912

    Purpose

    Encodes the spCas9 UAS (miniCMV core) gRNA expression cassette as a type 234 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    sgRNA-pUAS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. MTK234_041

    Plasmid

    #123916

    Purpose

    Encodes the spCas9 mScarlet gRNA expression cassette as a type 234 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    mScarlet sgRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPN177

    Plasmid

    #91628

    Purpose

    Express sgRNA targeting human IDO2

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    IDO2 (IDO2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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