We narrowed to 7,723 results for: ALP

1. pSNAP-CAPZ

   Plasmid

   #69948

   Purpose

   Simultaneous expression of a His- and SNAP-tagged β1 subunit and untagged α1 subunits of chicken CapZ

   Depositor

   Jeff Gelles

   Article

   Bombardier et al Nat Commun. 2015 Nov 13;6:8707. doi: 10.

   Inserts

   CapZ beta 1 (CAPZB Chicken)
   CapZ alpha 1 (CAPZA1 Chicken)
   

   Tags

   His 6 and SNAP

   Expression

   Bacterial

   Available Since

   Nov. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. NbPT5b-p3

   Plasmid

   #160001

   Purpose

   Multigene construct containing the betalain pigment biosynthetic genes under the control of the NbPT5b promoter for visualisation of arbuscular mycorrhizal colonisation in Nicotiana benthamiana roots.

   Depositor

   Sam Brockington

   Article

   Timoneda et al PLoS Biol. 2021 Jul 14;19(7):e3001326. d

   Inserts

   Bar (reverse orientation)
   DODAα1
   CYP76AD1
   cDOPA5GT
   

   Expression

   Plant

   Promoter

   Agrobacterium tumefaciens Nopaline synthase Promo…

   Available Since

   July 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. NbBCP1b-p3

   Plasmid

   #160002

   Purpose

   Multigene construct containing the betalain pigment biosynthetic genes under the control of the NbBCP1b promoter for visualisation of arbuscular mycorrhizal colonisation in Nicotiana benthamiana roots

   Depositor

   Sam Brockington

   Article

   Timoneda et al PLoS Biol. 2021 Jul 14;19(7):e3001326. d

   Inserts

   Bar (reverse orientation)
   DODAα1
   CYP76AD1
   cDOPA5GT
   

   Expression

   Plant

   Promoter

   Agrobacterium tumefaciens Nopaline synthase Promo…

   Available Since

   July 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA-GFP-EphrinB3-Flag(A227Y)

   Plasmid

   #155013

   Purpose

   Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EphrinB3 A227Y variant from pcDNA3.1

   Depositor

   Sinisa Urban

   Article

   Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

   Insert

   EphrinB3 (EPHB3 Human)
   

   Tags

   Flag, GFP, and signal peptide (residues 1 to 32) …

   Expression

   Mammalian

   Mutation

   A227Y

   Promoter

   CMV

   Available Since

   Nov. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. INS-GFP-CD19-EPT

   Plasmid

   #210469

   Purpose

   Donor plasmid for knock-in GFP-CD19 into the human INS locus

   Depositor

   Kai Wang

   Article

   Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

   Insert

   INS homologous recombination arms with a GFP-CD19 and EF1alpha-drived puromycin-NLS-tdTomato selection cassette (INS Human)
   

   Use

   CRISPR

   Available Since

   Jan. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. ADRA1D-Tango

   Plasmid

   #66215

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   ADRA1D (ADRA1D Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

7. TFORF0075

   Plasmid

   #142448

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   KCNIP4 (KCNIP4 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   June 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. human a5 full length

   Plasmid

   #217818

   Purpose

   Full length human integrin a5 expression

   Depositor

   Timothy Springer

   Article

   Yuxin et al bioRxiv 2024.01.26.577394

   Insert

   ITGA5 (ITGA5 Human)
   

   Tags

   P2A-EGFP

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1 NLRP1-delPYD-delCARD-TEV-GFP-FLAG codon-optimized

   Plasmid

   #166834

   Purpose

   Expression and purification of NLRP1 (without the PYD and CARD) from mammalian cells

   Depositor

   Hao Wu

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   NLRP1 (NLRP1 Human)
   

   Tags

   TEV-GFP-FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. PLL5.0-Anillin ShRNA-GFP-AnillinFL

    Plasmid

    #187272

    Purpose

    Lentiviral expression of shRNA targeting Anillin + reconstitution of Anillin full length

    Depositor

    Alpha Yap

    Article

    Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

    Insert

    Anillin ,hsAnillin, Entrez Gene ANLN (a.k.a. FSGS8, Scraps, scra) (ANLN Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Promoter

    U6, CMV

    Available Since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRRLNeo-pEF1a-p53ashL344P-mCerulean

    Plasmid

    #69581

    Purpose

    Expresses p53-L344P mutant tagged with mCerulean

    Depositor

    Galit Lahav

    Article

    Gaglia et al Proc Natl Acad Sci U S A. 2013 Sep 17;11

    Insert

    p53-L344P (TP53 Human)
    

    Use

    Lentiviral

    Tags

    mCerulean

    Expression

    Mammalian

    Mutation

    p53 L344P mutation that prevents oliogomerization…

    Promoter

    EF1alpha

    Available Since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. Cav2.1 EA2, R57A, R59A pMT2

    Plasmid

    #206122

    Purpose

    expression of rabbit Cav2.1 calcium channel with a CaV2.1-P1217fs mutation causing a truncated protein, (EA2). R57A R59A mutations prevent dominant-negative suppression of Cav2.1 currents

    Depositor

    Annette Dolphin

    Article

    Dahimene et al Neurobiol Dis. 2016 Sep;93:243-56. doi:

    Insert

    cacna1a (Cacna1a Rat)
    

    Expression

    Mammalian

    Mutation

    Cav2.1-P1217fs, R57A, R59A

    Promoter

    Ad MLP/TPL/SV40

    Available Since

    Nov. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMH012_AAVS1_puro-pA-9xTetO-pEF-H2B-mCitrine-core-pRSV-H2B-mCherry

    Plasmid

    #179435

    Purpose

    Dual fluorescent reporter construct with single core HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, arms for integration at AAVS1 locus

    Depositor

    Lacra Bintu

    Article

    Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

    Insert

    TRE-cit-coreHS4-mch (SC)
    

    Expression

    Mammalian

    Promoter

    pEF alpha, pRSV

    Available Since

    Feb. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28a-CTLA4-FLN-ELP-His-ybbr

    Plasmid

    #168038

    Purpose

    Expresses CTLA-4 with FLN fingerprint domain, ELP linker, 6x-His tag and ybbr tag at the C terminus

    Depositor

    Michael Nash

    Article

    Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

    Insert

    Cytotoxic T-lymphocyte associated protein 4 (CTLA4 Human)
    

    Tags

    3x ELP linker, 6x Histag, ddFLN4 (C18S), and ybbr…

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEGB SF-35S:Renilla:TNOS-35S:P19:TNOS (GB0160)

    Plasmid

    #75412

    Purpose

    Module for the expression of the Renilla Luciferase with the silencing suppressor P19

    Depositor

    Diego Orzaez

    Article

    Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

    Insert

    Renilla / P19
    

    Use

    Luciferase and Synthetic Biology

    Expression

    Plant

    Promoter

    35S

    Available Since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. TFORF3405

    Plasmid

    #144881

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    KCNIP4 (KCNIP4 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. TFORF0077

    Plasmid

    #142450

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    KCNIP4 (KCNIP4 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    June 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. TFORF0076

    Plasmid

    #142449

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    KCNIP4 (KCNIP4 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    June 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. TFORF0078

    Plasmid

    #141507

    Purpose

    Lentiviral vector for overexpressing the KCNIP4 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    KCNIP4 (KCNIP4 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. FSS-GFP

    Plasmid

    #163654

    Purpose

    Expresses a TFR and ST6GAL1 swapped chimera with the cytosolic tail from TFR and TMD and luminal domain from ST6GAL1 in mammalian cells

    Depositor

    Lei Lu

    Article

    Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

    Insert

    ST6 beta-galactoside alpha-2,6-sialyltransferase 1 and transferrin receptor (ST6GAL1, TFRC Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    This chimera contains the cytosolic tail from TFR…

    Available Since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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