We narrowed to 10,634 results for: SEC

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pWalium20-10XUAS-3XFLAG-dCas9-VPR

Plasmid

#78897

Purpose

Expresses 3XFLAG-dCas9-VPR (human codon) under 10XUAS control, for in vivo CRISPRa

Depositor

Norbert Perrimon

Article

Lin et al Genetics. 2015 Oct;201(2):433-42. doi: 1

Insert

dCas9-VPR

Tags

3xFLAG

Expression

Insect

Promoter

10XUAS

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pCoofy44

Plasmid

#55185

Purpose

Insect expression vector for parallel SLIC cloning containing N-terminal 10x His and SumoStar tags. C-terminal tags can be added during SLIC.

Depositor

Sabine Suppmann

Article

Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

Type

Empty backbone

Tags

10x His, 10x His-Stop-OneStrep-Stop-S-tag-Stop-HP…

Expression

Bacterial

Promoter

pPH (Polyhedrin promoter)

Available Since

July 23, 2014

Availability

Academic Institutions and Nonprofits only

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pFastBac TwinStrepII Prescission SNAPf TEV cloning vector with BioBrick PolyPromoter LIC Subcloning (438-SNAP-V1)

Plasmid

#55222

Depositor

Scott Gradia

Article

Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

Type

Empty backbone

Tags

SNAPf and TwinStrepII

Expression

Insect

Available Since

June 17, 2014

Availability

Academic Institutions and Nonprofits only

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pENTR R4-vas2-integrase-R3

Plasmid

#62299

Purpose

phageC31 integrase-expressing helper plasmid for Anopheles transgenesis, vasa promoter

Depositor

Eric Marois

Article

Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

Insert

phageC31 integrase under control of vasa promoter and terminator

Tags

HA and SV40 NLS

Expression

Insect

Promoter

vasa

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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pF3BGX-7xGFP11

Plasmid

#138397

Purpose

RMCE vector for generating C-terminal fusion of seven tandem GFP11 tags (split GFP)

Depositor

Shu Kondo

Article

Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

Insert

7xGFP11

Expression

Insect

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pAct-PE2-HygroR

Plasmid

#149552

Purpose

Expression of PE2 enzyme under control of ubiquitous Actin5c promoter. Can be used to generate stable cell lines by Hygromycin selection.

Depositor

Norbert Perrimon

Article

Bosch et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

Insert

PE2

Expression

Insect

Promoter

Act5c

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pFastBac1 INI1

Plasmid

#1953

Depositor

Robert Kingston

Article

Phelan et al Mol Cell 1999 Feb;3(2):247-53.

Insert

INI1 (SMARCB1 Human)

Tags

flag

Expression

Insect

Available Since

May 24, 2006

Availability

Academic Institutions and Nonprofits only
pUASP dsRed Rab7 WT

Plasmid

#44824

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab7 (Rab7 Fly)

Use

Drosophila

Tags

dsRed

Expression

Insect

Available Since

April 18, 2013

Availability

Academic Institutions and Nonprofits only
pTriEx-mCherry-LOV2 I427T

Plasmid

#81035

Purpose

Mammalian expression of LOV I427T, a mutant with super short relaxation time

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

LOV I427T

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Mutation

I427T

Available Since

Aug. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pTriExEKAR2G2

Plasmid

#40175

Depositor

Olivier Pertz

Article

Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

Insert

Erk activity reporter

Tags

His-Myc

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-3xP3-RFP

Plasmid

#130276

Purpose

CRISPaint universal donor plasmid for gene exon insertion. Encodes 3xP3-RFP visible eye marker and multiple cloning site.

Depositor

Norbert Perrimon

Article

Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

Insert

3xP3-RFP

Use

CRISPR

Expression

Insect

Available Since

Aug. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pKanB alpha

Plasmid

#223524

Purpose

Used for expression of secreted recombinant protein in Pichia pastoris; with G418 resistance gene as selectable marker

Depositor

Geoff Lin-Cereghino

Article

Lin-Cereghino et al Yeast. 2008 Apr;25(4):293-9. doi: 10.100

Type

Empty backbone

Expression

Yeast

Promoter

AOX1

Available Since

Sept. 11, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBD-SpyTag-noro-VLP

Plasmid

#165989

Purpose

Expresses norovirus-like particles with SpyTags presented on their surface for convenient decoration of SpyCatcher fusion proteins

Depositor

Vesa Hytönen

Article

Lampinen et al J Nanobiotechnology. 2021 Jan 19;19(1):2

Insert

SpyTag-noro-VLP (ORF2 norovirus, Synthetic)

Tags

SpyTag

Expression

Insect

Promoter

Polyhedrin

Available Since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-3xP3-GFP

Plasmid

#130277

Purpose

CRISPaint universal donor plasmid for gene exon insertion. Encodes 3xP3-GFP visible eye marker and multiple cloning site.

Depositor

Norbert Perrimon

Article

Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

Insert

3xP3-GFP

Use

CRISPR

Expression

Insect

Available Since

Aug. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pKM3

Plasmid

#154168

Purpose

Empty vector with FLAG tag expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus.

Depositor

Kevin Maringer

Article

Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

Type

Empty backbone

Expression

Insect

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pJC-20xUAS-TALE3-VP64-P10

Plasmid

#104607

Purpose

Expresses TALE3 under UAS control

Depositor

Tudor Fulga

Article

Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

Insert

TALE3

Use

TALEN

Expression

Insect

Promoter

hsp70

Available Since

Jan. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pJC-20xUAS-TALE4-VP64-P10

Plasmid

#104608

Purpose

Expresses TALE4 under UAS control

Depositor

Tudor Fulga

Article

Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

Insert

TALE4

Use

TALEN

Expression

Insect

Promoter

hsp70

Available Since

Jan. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pFasBac+GFP-CAMSAP1

Plasmid

#59036

Purpose

for baculovirus expression of CAMSAP1 in Sf9 cells

Depositor

Ron Vale

Article

Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

Insert

CAMSAP1 (CAMSAP1 Human)

Tags

His and eGFP

Expression

Insect

Available Since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pHACK-GAL4>QF2(newHA1)

Plasmid

#104873

Purpose

HACK Donor plasmids for converting GAL4 lines to QF2 lines using HACK method. Updated version of Addgene#80275 for easier cloning of inserts

Depositor

Christopher Potter

Article

Lin et al Genetics. 2016 Jun 22. pii: genetics.116

Insert

T2A-QF2

Use

CRISPR

Expression

Insect

Available Since

March 12, 2018

Availability

Academic Institutions and Nonprofits only

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p{CFD4-EYFP-3xP3::DsRed}

Plasmid

#86863

Purpose

Expresses an EYFP gRNA ubiquitously and DsRed in eyes as a marker. With attB integration site.

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2017 Apr 3;7(4):1339-1347

Type

Empty backbone

Expression

Insect

Available Since

Feb. 24, 2017

Availability

Academic Institutions and Nonprofits only

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