We narrowed to 7,679 results for: URE
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Plasmid#162654PurposeExpresses ORF68 K174A/R179A/K182A in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage siteDepositorInsertORF68
TagsN-terminal 2xStrep-Tag II and HRV 3C siteExpressionMammalianMutationAmino acids K174, R179, and K182 mutated to alani…PromoterCMVAvailable SinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVRC8400-SARS-CoV-2-RBD-L455RG496R-AVI
Plasmid#160479PurposeExpresses SARS-CoV-2 RBD-L455RG496R domain with single chain Fc, HRV3C protease cleavage site, and AVI tagDepositorInsertSARS-CoV-2-RBD-L455RG496R
TagsAVI, HRV3C, and scFcExpressionMammalianMutationchanged Leucine 455 to Arginine and changed Glyci…Available SinceOct. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVRC8400-SARS-CoV-2-RBD-L455RA475R-AVI
Plasmid#160478PurposeExpresses SARS-CoV-2 RBD-L455RA475R domain with single chain Fc, HRV3C protease cleavage site, and AVI tagDepositorInsertSARS-CoV-2-RBD-L455RA475R
TagsAVI, HRV3C, and scFcExpressionMammalianMutationchanged Leucine 455 to Arginine and changed Alani…Available SinceOct. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDF11-SFPQ(214-598)_quadruple_mutant
Plasmid#135441PurposeExpresses SFPQ 214-598 quadruple mutant (L535A, L539A, L546A, M549A) with TEV-cleavable hexahistidine tagDepositorInsertsplicing factor proline and glutamine rich (SFPQ Human)
TagsHexahistidineExpressionBacterialMutationL535A, L539A, L546A, M549APromoterT7Available SinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAD100_hIRG1_4-461_pvp008
Plasmid#124875PurposeExpresses hCAD Arg273His mutant in E.coliDepositorInsertcis-aconitate decarboxylase (ACOD1 Human)
TagsStrepTag and TEV siteExpressionBacterialMutationmutated Arg273 to His, deleted amino acids 1-3 an…PromoterT7Available SinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-TIRAP-cGASΔN-HA
Plasmid#130921PurposeExpresses human cGASΔN (aa160-522)-HA with an N terminal fusion of the TIRAP N terminus (aa1-85); Puromycin selection markerDepositorInsertTIRAP-ΔNcGAS
TagsHAExpressionMammalianMutationcGAS N terminus (aa1-159) replaced with the N ter…PromoterCMVAvailable SinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV hSyn1 CaRhAC T2A tDimer
Plasmid#101722Purpose- humanized - variant of pAAV hSyn YFP-CaRhAC Addgene # 101721– less reliable in hippocampal neuronsDepositorInsertsCatRhAC
red fluorescent protein
UseAAVMutationE497K,C569DPromoterhuman Synapsin1 promotor and ribosomal skip seque…Available SinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
GST-PRMT9 deltaC 350 (stop)
Plasmid#67612Purposebacterial expression of GST-PRMT9 missing residues 351-845DepositorInsertPRMT9 (PRMT9 Human)
TagsGSTExpressionBacterialMutationStop codons placed at aa 351 and 352 to create C …PromotertacAvailable SinceJuly 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT/TO-GFP-I3(V41A/W43A)
Plasmid#61402PurposeExpresses GFP-tagged, PP1 binding-deficient Inhibitor-3 in mammalian cellsDepositorAvailable SinceSept. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMT mir-1-1 reporter cel-mir-50
Plasmid#46740DepositorInsertcel-mir-50
UseRNAiTagsHis and hsa-mir-1-1ExpressionInsectPromoterMetallothionein (MT)Available SinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMT mir-1-1 reporter cel-lsy-6
Plasmid#46738DepositorInsertcel-lsy-6
UseRNAiTagsHis and hsa-mir-1-1ExpressionInsectPromoterMetallothionein (MT)Available SinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMT mir-1-1 reporter cel-lin-4
Plasmid#46737DepositorInsertcel-lin-4
UseRNAiTagsHis and hsa-mir-1-1ExpressionInsectPromoterMetallothionein (MT)Available SinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
SRAcode_in_pUC57_v3(419-999)
Plasmid#49824Purposefor transcribing SRA nucl 419-999 of NM_001035235 with T7DepositorInsertsteroid receptor RNA activator RNA coding (SRA1 Human)
ExpressionBacterialMutationKpnI site at 5' start of RNA coding seqPromoterT7Available SinceJan. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
SRAcode_in_pUC57_v3(767-999)
Plasmid#49825Purposefor transcribing SRA nucl 767-999 of NM_001035235 with T7DepositorInsertsteroid receptor RNA activator RNA coding (SRA1 Human)
ExpressionBacterialMutationKpnI site at 5' start of RNA coding seqPromoterT7Available SinceJan. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
SRAcode_in_pUC57_v3(419-771)
Plasmid#49823Purposefor transcribing SRA nucl 419-771 of NM_001035235 with T7DepositorInsertsteroid receptor RNA activator RNA coding (SRA1 Human)
ExpressionBacterialMutationKpnI site at 5' start of RNA coding seqPromoterT7Available SinceJan. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTYB1-pLAC-N71
Plasmid#48720PurposeBacterial expression of Lacritin protein with 70 AA removed from the N-terminusDepositorInsertLacritin (LACRT Human)
TagsInteinExpressionBacterialMutationDeleted first 71 amino acids of mature lacritin w…PromoterT7Available SinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTYB1-pLAC-N45
Plasmid#48718PurposeBacterial expression of Lacritin protein with 45 AA removed from N terminusDepositorInsertLacritin (LACRT Human)
TagsInteinExpressionBacterialMutationDeleted first 45 amino acids of mature lacritin w…Available SinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
HLH Bmal Pas 1-465-VenC F423R/V435R
Plasmid#47371PurposeBmal fragment cloned with Venus tag used for mutationDepositorInsertBMAL (Bmal1 Mouse)
TagsVenus aa 156-239ExpressionMammalianMutationaa 1-465, F423R/V435RPromoterCMVAvailable SinceSept. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMB38-HF380deltaMTBD
Plasmid#37333DepositorInsertCytoplasmic Dynein Heavy Chain (dhcA Dictyostelium discoideum)
UseDictyostelium expressionTagsHis6 and FLAG tagMutationdeleted amino acids 1-1387 and replaced amino aci…PromoterTRE-PminAvailable SinceJan. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJLI-Sup35N-KDG6-C
Plasmid#1238DepositorInsertSUP35 (SUP35 Budding Yeast)
UseYeast integrative plasmidMutationSUP35 middle region (aa124-253) was replaced with…Available SinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only