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pWS936

Plasmid

#123050

Purpose

G_ subunit for creating C-terminal transplants

Depositor

Tom Ellis

Publication

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

Gpa1 C-terminal Truncation Vector [3-4a]

Expression

Yeast

Available since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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pDM1220

Plasmid

#108993

Purpose

integrating expression vector

Depositor

Rob Kay

Publication

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Dictyostelium discoideum integrating expression v…

Available since

July 18, 2018

Availability

Academic Institutions and Nonprofits only

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pTC440

Plasmid

#91225

Purpose

protoplast vector expressing gRNA38 targeting barley MLO (control without TREX2 expression)

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

gRNA targeting barley MLO

Use

CRISPR

Expression

Plant

Available since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pTC391

Plasmid

#91220

Purpose

protoplast vector expressing gRNA23 targeting tomato ANT1 (with TREX2 expression)

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

gRNA targeting tomato ANT1

Use

CRISPR

Expression

Plant

Available since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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pTC394

Plasmid

#91224

Purpose

protoplast vector expressing gRNA24 targeting tomato ANT1 (control without TREX2 expression)

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

gRNA targeting tomato ANT1

Use

CRISPR

Expression

Plant

Available since

July 11, 2017

Availability

Academic Institutions and Nonprofits only

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pTC392

Plasmid

#91223

Purpose

protoplast vector expressing gRNA23 targeting tomato ANT1 (control without TREX2 expression)

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

gRNA targeting tomato ANT1

Use

CRISPR

Expression

Plant

Available since

July 11, 2017

Availability

Academic Institutions and Nonprofits only

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pTC436

Plasmid

#91222

Purpose

protoplast vector expressing gRNA38 targeting barley MLO (with TREX2 expression)

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

gRNA targeting barley MLO

Use

CRISPR

Expression

Plant

Available since

July 11, 2017

Availability

Academic Institutions and Nonprofits only

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pTC393

Plasmid

#91221

Purpose

protoplast vector expressing gRNA24 targeting tomato ANT1 (with TREX2 expression)

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

gRNA targeting tomato ANT1

Use

CRISPR

Expression

Plant

Available since

July 11, 2017

Availability

Academic Institutions and Nonprofits only

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pMOD_A1410

Plasmid

#91035

Purpose

Module A, Promoter: ZmUbi, Gene: TaCas9_dead (D10A + H840A), Terminator: HSP

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

TaCas9_dead (D10A + H840A)

Use

CRISPR

Mutation

D10A, H840A

Promoter

ZmUbi

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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2xNLS-pMJ915v2-sfGFP

Plasmid

#88920

Purpose

For expression of modified SpyCas9 in e.coli.

Depositor

Jennifer Doudna

Publication

Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Inserts

Cas9
T7

Use

CRISPR

Expression

Bacterial

Available since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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1xNLS-pMJ915v2-sfGFP

Plasmid

#88919

Purpose

For expression of modified SpyCas9 in e.coli.

Depositor

Jennifer Doudna

Publication

Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Inserts

Cas9
T7

Use

CRISPR

Expression

Bacterial

Available since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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pGGD008

Plasmid

#48838

Purpose

Provides an ER retrieval signal as GreenGate C-terminal tag module.

Depositor

Jan Lohmann

Publication

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

ER retrieval signal

Use

Golden gate compatible cloning vector

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pEvol sfGFP WT

Plasmid

#253740

Purpose

WT reporter plasmid

Depositor

Abhishek Chatterjee

Publication

Soni et al ACS Cent Sci. 2024 May 20;10(6):1211-122

Insert

sfGFP

Tags

6xHis tag

Expression

Bacterial

Promoter

T5-lac

Available since

June 17, 2026

Availability

Academic Institutions and Nonprofits only

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pTW2657

Plasmid

#252577

Purpose

TRV2 multiplex vector expressing Ymu1-WFR, AtPDS3 gRNA12 and AtCHLl1 gRNA4

Depositor

Steven Jacobsen

Publication

Weiss et al Plant Biotechnol J. 2026 Mar 17. doi: 10

Insert

Ymu1-WFR with AtPDS3 gRNA 12 and AtCHLl1 gRNA4

Use

CRISPR

Expression

Plant

Available since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_NTC2

Plasmid

#183334

Purpose

All-in-One CRISPRko system containing a non-targeting control

Depositor

Prashant Mali

Publication

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

NTC2

Use

Lentiviral

Available since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pTRE3G-Fluc-DR

Plasmid

#242434

Purpose

Firefly Luciferse with a Direct Repeat sequence in the 3’UTR recognised by the CasRx under the control of the TRE3G promoter.

Depositor

Diego Di Bernardo

Publication

De Carluccio et al Nat Commun. 2024 Apr 17;15(1):3311. doi:

Insert

Firefly Luciferase

Use

CRISPR and Luciferase

Tags

CasRx Direct Repeat

Available since

Nov. 11, 2025

Availability

Academic Institutions and Nonprofits only

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TwinStrepSUMO-scFv-SpyCatcher

Plasmid

#243753

Purpose

For affinity purification of anti-HA scFv fused to SpyCatcher

Depositor

Feng Zhang

Publication

Kreitz et al Nat Biotechnol. 2025 Aug 12. doi: 10.103

Insert

scFv-SpyCatcher

Expression

Bacterial

Available since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pBR322-sgRNA-pvc18-20

Plasmid

#243747

Purpose

PVC cargo/regulatory region - deleted toxin cargos (Pdp1/Pnf) - vegfa sgRNA

Depositor

Feng Zhang

Publication

Kreitz et al Nat Biotechnol. 2025 Aug 12. doi: 10.103

Insert

vegfa sgRNA-pvc18-20

Expression

Bacterial

Available since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_pvc12-FLAG_Δpvc10

Plasmid

#243738

Purpose

PVC structural operon - FLAG-tagged baseplate (Pvc12) - deleted spike tip (Pvc10)

Depositor

Feng Zhang

Publication

Kreitz et al Nat Biotechnol. 2025 Aug 12. doi: 10.103

Insert

pvc12-FLAG_ Δpvc10

Expression

Bacterial

Available since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pGWB514-35S:SynVrFLS2_22-HA

Plasmid

#226418

Purpose

Expressing SynVrFLS2_22 protein in plants

Depositor

Gitta Coaker

Publication

Li et al Nat Plants. 2025 Aug;11(8):1628-1641. do

Insert

SynVrFLS2_22

Tags

HA

Expression

Plant

Promoter

35S

Available since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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