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Plasmid#102870PurposeExpresses mouse Fzd8 in mammalian cellsDepositorInsertFrizzled 8 (Fzd8 Mouse)
UseLentiviralTagsExpressionMammalianMutationT to C substitution at nucleotide 1134, C to T su…PromoterEF1AAvailable sinceJan. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCBh-T7-SpG-P2A-mTagBFP2(RAS3583)
Plasmid#223110PurposepCBh Human expression plasmid for SpG with with C-term BPNLS-P2A-mTagBFP2DepositorInserthuman codon optimized nuclease SpG-P2A-mTagBFP2
UseCRISPRTagsBPNLS-3xFLAG-P2A-mTagBFP2ExpressionMammalianMutationSpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337RPromoterCMV and T7Available sinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCBh-T7-SpCas9-P2A-mTagBFP2(RAS3575)
Plasmid#223111PurposepCBh Human expression plasmid for SpCas9 with with C-term BPNLS-P2A-mTagBFP2DepositorInserthuman codon optimized nuclease SpCas9-P2A-mTagBFP2
UseCRISPRTagsBPNLS-3xFLAG-P2A-mTagBFP2ExpressionMammalianMutationPromoterCMV and T7Available sinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-BPNLS-CE-ABE8e-SpCas9(D10A)-BPNLS-P2A-EGFP (NK350)
Plasmid#208292PurposeCMV and T7 promoter expression plasmid for human codon optimized CE-ABE8e-SpCas9 A-to-G base editor and P2A-EGFPDepositorInsertpCMV-T7-BPNLS-CE-ABE8e-SpCas9-BPNLS-P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLS and BPNLS-P2A-EGFPExpressionMammalianMutationABE8e mutations in TadA inlaid into nSpCas9(D10A)PromoterCMV and T7Available sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-FLEX-jGCaMP7c variant 1513-WPRE (AAV1)
Viral Prep#105322-AAV1PurposeReady-to-use AAV1 particles produced from pGP-AAV-syn-FLEX-jGCaMP7c variant 1513-WPRE (#105322). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP7c variant 1513-WPRE plasmid DNA. Synapsin-driven, Cre-dependent jGCaMP7c expression. jGCaMP7c exhibits high contrast between peak fluorescence and resting fluorescence. It is useful for activity imaging of large populations of densely-labeled neurons because background fluorescence from inactive neurons is reduced. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsNoneAvailable sinceJune 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-jGCaMP7c variant 1513-WPRE (AAV1)
Viral Prep#105321-AAV1PurposeReady-to-use AAV1 particles produced from pGP-AAV-syn-jGCaMP7c variant 1513-WPRE (#105321). In addition to the viral particles, you will also receive purified pGP-AAV-syn-jGCaMP7c variant 1513-WPRE plasmid DNA. Synapsin-driven jGCaMP7c expression. jGCaMP7c exhibits high contrast between peak fluorescence and resting fluorescence. It is useful for activity imaging of large populations of densely-labeled neurons because background fluorescence from inactive neurons is reduced. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsNoneAvailable sinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-FLEX-jGCaMP7b-WPRE (AAV Retrograde)
Viral Prep#104493-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pGP-AAV-syn-FLEX-jGCaMP7b-WPRE (#104493). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP7b-WPRE plasmid DNA. Synapsin-driven, Cre-dependent jGCaMP7b expression. jGCaMP7b expression exhibits the brightest resting fluorescence and can be used for imaging of small neuronal processes (dendrites and axons). These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsNoneAvailable sinceNov. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJK681
Plasmid#72271PurposeProduces Synechocystis PCC6803 orf sll1489 (synthetic gene)DepositorInserthypothetical protein SYNGTS_3005
UseTagsHis6 and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceFeb. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK588
Plasmid#72270PurposeSynthetic gene corresponding to Synechocystis PCC6803 orf sll1489DepositorInserthypothetical protein SYNGTS_3005
UseGene cloningTagsHis6 and TEV cleavage siteExpressionMutationPromoterT7Available sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-SpCas9(LWKYQS)-P2A-EGFP (AHK34)
Plasmid#223079PurposepCMV and pT7 Human expression plasmid for SpCas9 enzyme with LWKYQS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFPDepositorInserthuman codon optimized nuclease SpCas9(LWKYQS)-P2A-EGFP
UseCRISPRTagsBPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationSpCas9(LWKYQS)=D1135L, S1136W, G1218K, E1219Y, R1…PromoterCMV and T7Available sinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-SpCas9(MRKCRS)-P2A-EGFP (RAS2821)
Plasmid#223102PurposepCMV and pT7 Human expression plasmid for SpCas9 enzyme with MRKCRS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFPDepositorInserthuman codon optimized nuclease SpCas9(MRKCRS)-P2A-EGFP
UseCRISPRTagsBPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationSpCas9(MRKCRS)=D1135M, S1136R, G1218K, E1219C, R1…PromoterCMV and T7Available sinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-SpCas9(SRQMRG)-P2A-EGFP (RAS3017)
Plasmid#223103PurposepCMV and pT7 Human expression plasmid for SpCas9 enzyme with SRQMRG amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFPDepositorInserthuman codon optimized nuclease SpCas9(SRQMRG)-P2A-EGFP
UseCRISPRTagsBPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationSpCas9(SRQMRG)=D1135S, S1136R, G1218Q, E1219M, R1…PromoterCMV and T7Available sinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-SpCas9(MRKQKC)-P2A-EGFP (RAS2838)
Plasmid#223104PurposepCMV and pT7 Human expression plasmid for SpCas9 enzyme with MRKQKC amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFPDepositorInserthuman codon optimized nuclease SpCas9(MRKQKC)-P2A-EGFP
UseCRISPRTagsBPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationSpCas9(MRKQKC)=D1135M, S1136R, G1218K, E1219Q, R1…PromoterCMV and T7Available sinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-SpCas9(MRKSKC)-P2A-EGFP (RAS2840)
Plasmid#223105PurposepCMV and pT7 Human expression plasmid for SpCas9 enzyme with MRKSKC amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFPDepositorInserthuman codon optimized nuclease SpCas9(MRKSKC)-P2A-EGFP
UseCRISPRTagsBPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationSpCas9(MRKSKC)=D1135M, S1136R, G1218K, E1219S, R1…PromoterCMV and T7Available sinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCBh-T7-SpCas9(KRTMQR)-P2A-mTagBFP2(RAS3591)
Plasmid#223106PurposepCBh Human expression plasmid for SpCas9 enzyme with KRTMQR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-mTagBFP2DepositorInserthuman codon optimized nuclease SpCas9(KRTMQR)-P2A-mTagBFP2
UseCRISPRTagsBPNLS-3xFLAG-P2A-mTagBFP2ExpressionMammalianMutationSpCas9(KRTMQR)=D1135K, S1136R, G1218T, E1219M, R1…PromoterCMV and T7Available sinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCBh-T7-SpCas9(KRHWMR)-P2A-mTagBFP2(RAS3594)
Plasmid#223107PurposepCBh Human expression plasmid for SpCas9 enzyme with KRHWMR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-mTagBFP2DepositorInserthuman codon optimized nuclease SpCas9(KRHWMR)-P2A-mTagBFP2
UseCRISPRTagsBPNLS-3xFLAG-P2A-mTagBFP2ExpressionMammalianMutationSpCas9(KRHWMR)=D1135K, S1136R, G1218H, E1219W, R1…PromoterCMV and T7Available sinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCBh-T7-SpCas9(MRRWYR)-P2A-mTagBFP2(RAS3598)
Plasmid#223108PurposepCBh Human expression plasmid for SpCas9 enzyme with MRRWYR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-mTagBFP2DepositorInserthuman codon optimized nuclease SpCas9(MRRWYR)-P2A-mTagBFP2
UseCRISPRTagsBPNLS-3xFLAG-P2A-mTagBFP2ExpressionMammalianMutationSpCas9(MRRWYR)=D1135M, S1136R, G1218R, E1219W, R1…PromoterCMV and T7Available sinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCBh-T7-SpCas9(MRRWMR)-P2A-mTagBFP2(RAS3579)
Plasmid#223109PurposepCBh Human expression plasmid for SpCas9 enzyme with MRRWMR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-mTagBFP2DepositorInserthuman codon optimized nuclease SpCas9(MRRWMR)-P2A-mTagBFP2
UseCRISPRTagsBPNLS-3xFLAG-P2A-mTagBFP2ExpressionMammalianMutationSpCas9(MRRWMR)=D1135M, S1136R, G1218R, E1219W, R1…PromoterCMV and T7Available sinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-SpCas9(KWKYES)-P2A-EGFP (RAS2900)
Plasmid#223095PurposepCMV and pT7 Human expression plasmid for SpCas9 enzyme with KWKYES amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFPDepositorInserthuman codon optimized nuclease SpCas9(KWKYES)-P2A-EGFP
UseCRISPRTagsBPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationSpCas9(KWKYES)=D1135K, S1136W, G1218K, E1219Y, R1…PromoterCMV and T7Available sinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-SpCas9(LWKYEY)-P2A-EGFP (RAS3001)
Plasmid#223096PurposepCMV and pT7 Human expression plasmid for SpCas9 enzyme with LWKYEY amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFPDepositorInserthuman codon optimized nuclease SpCas9(LWKYEY)-P2A-EGFP
UseCRISPRTagsBPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationSpCas9(LWKYEY)=D1135L, S1136W, G1218K, E1219Y, R1…PromoterCMV and T7Available sinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only