We narrowed to 5,094 results for: p2a

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LV_EF1a_CDK1-P2A-Hygro_Barcode

Plasmid

#170221

Purpose

Barcoded lentiviral vector to express CDK1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

Depositor

Prashant Mali

Article

Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

Insert

CDK1 (CDK1 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pH3R-P2A-mCherry-N1

Plasmid

#84332

Purpose

Co-expresses (untagged) Histamine-3 receptor and mCherry

Depositor

Dorus Gadella

Article

van Unen et al Mol Pharmacol. 2016 Sep;90(3):162-76. do

Insert

H3R-P2A-mCherry (HRH3 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX-4T_CIP2A_1-560

Plasmid

#217921

Purpose

E. coli expression of GST-tagged CIP2A_1-560 protein

Depositor

Jukka Westermarck

Insert

CIP2A (CIP2A Human)

Tags

GST

Expression

Bacterial

Available Since

April 25, 2024

Availability

Academic Institutions and Nonprofits only

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LPA2R-P2A-mCherry

Plasmid

#112932

Purpose

Plasmid co-producing the LPA2 receptor and a mCherry

Depositor

Dorus Gadella

Article

Mastop et al PLoS One. 2018 Mar 5;13(3):e0193705. doi

Insert

LPA2 receptor

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pLJM1-Rap2A-3xFLAG

Plasmid

#87973

Purpose

Expression of Rap2A in mammalian cells

Depositor

David Sabatini

Article

Cantor et al Cell. 2017 Apr 6;169(2):258-272.e17. doi

Insert

RAP2A (RAP2A Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

April 18, 2017

Availability

Academic Institutions and Nonprofits only

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MS2392.EF1a.SLC6A1.WT.P2A.BLAST.JL.p159

Plasmid

#170170

Purpose

Lentiviral Expression of EF1a.SLC6A1.WT

Depositor

Christopher Moxham

Insert

SLC6A1 (SLC6A1 Human)

Use

Lentiviral

Mutation

n/a

Available Since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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TOP2A G11.2 gRNA

Plasmid

#90914

Purpose

3rd generation lentiviral gRNA plasmid targeting human TOP2A

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

TOP2A (Guide Designation G11.2)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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mOrange-P2A-hKvb1

Plasmid

#154084

Purpose

Independent expression of mOrange and human Kvbeta 1 under human synapsin 1 promoter

Depositor

Michael Hoppa

Article

Cho et al Proc Natl Acad Sci U S A. 2020 Nov 24;11

Insert

mOrange-P2A-human Kvb1 (KCNAB1 Human, Synthetic)

Use

AAV

Expression

Mammalian

Promoter

human synapsin 1

Available Since

Nov. 2, 2020

Availability

Academic Institutions and Nonprofits only

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MS2392.EF1a.SLC6A1.WT.EGFP.P2A.BLAST.JL.p164

Plasmid

#170169

Purpose

Lentiviral Expression of EF1a.SLC6A1.WT.EGFP

Depositor

Christopher Moxham

Insert

SLC6A1 (SLC6A1 Human)

Use

Lentiviral

Mutation

n/a

Available Since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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SIN40C.TRE.dTomato.miR-125b-2.PGK.sfGFP.P2A.Tet3G

Plasmid

#169315

Purpose

Doxycycline-inducible expression of miR-125b-2 with dTomato as reporter fluorescent protein

Depositor

Jan-Henning Klusmann

Article

Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

Insert

miR-125b-2 (MIR125B2 Human)

Use

Lentiviral

Tags

dTomato

Promoter

TRE

Available Since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pLJC2-Rap2A-3xFLAG

Plasmid

#87974

Purpose

3rd generation lentiviral vector for Expression of Rap2A in mammalian cells

Depositor

David Sabatini

Article

Cantor et al Cell. 2017 Apr 6;169(2):258-272.e17. doi

Insert

RAP2A (RAP2A Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

April 18, 2017

Availability

Academic Institutions and Nonprofits only

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pLJC5-Rap2A-3xFLAG

Plasmid

#163444

Purpose

lentiviral overexpression vector for human Rap2A

Depositor

Jason Cantor

Article

Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

Insert

Rap2A (RAP2A Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

Ubc

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLJC6-Rap2A-3xFLAG

Plasmid

#163445

Purpose

lentiviral overexpression vector for human Rap2A

Depositor

Jason Cantor

Article

Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

Insert

Rap2A (RAP2A Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

Ubc

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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LV_EF1a_CDK6-P2A-Hygro_Barcode

Plasmid

#170224

Purpose

Barcoded lentiviral vector to express CDK6 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

Depositor

Prashant Mali

Article

Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

Insert

CDK6 (CDK6 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pLJC1-Rap2A-3xFLAG

Plasmid

#87972

Purpose

Expression of Rap2A in mammalian cells

Depositor

David Sabatini

Article

Cantor et al Cell. 2017 Apr 6;169(2):258-272.e17. doi

Insert

RAP2A (RAP2A Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

April 18, 2017

Availability

Academic Institutions and Nonprofits only

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AmCyan-P2A-Xerry

Plasmid

#48686

Purpose

Expresses AmCyan while the mCherry gene has been mutated

Depositor

Ilpo Huhtaniemi

Insert

AmCyan

Tags

mutant mCherry (truncated)

Expression

Mammalian

Mutation

SV40 NLS

Promoter

CMV

Available Since

Nov. 6, 2013

Availability

Academic Institutions and Nonprofits only

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QuasAr-P2A-GCaMP

Plasmid

#154082

Purpose

Independent expression of QuasAr and GCaMP under human synapsin 1 promoter

Depositor

Michael Hoppa

Article

Cho et al Proc Natl Acad Sci U S A. 2020 Nov 24;11

Insert

QuasAr-P2A-GCaMP6f

Use

AAV

Expression

Mammalian

Mutation

Changed Tyrosine 327 to Alanine

Promoter

human synapsin 1

Available Since

Nov. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pKF-P14MMP2AG-T2APuroR-5h

Plasmid

#128256

Purpose

Mammalian positive feedback gene circuit in a Flp-In expression system controlling the PuroR gene. Also called mPF-PuroR.

Depositor

Gabor Balazsi

Article

Farquhar et al Nat Commun. 2019 Jun 24;10(1):2766. doi:

Insert

rtTA-Advanced::P2A::EGFP::T2A::PuroR

Use

Synthetic Biology; Flp-in expression vector

Expression

Mammalian

Promoter

tight TRE promoter

Available Since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pBT2-4xUAS:P2A-mRFP

Plasmid

#127593

Purpose

This vector is used for cloning a gene driven by 4xnrUAS, to be co-expressed with mRFP through P2A self-cleaving peptides.

Depositor

Longhou Fang

Article

Gu et al Science. 2019 Mar 8;363(6431):1085-1088.

Type

Empty backbone

Expression

Mammalian

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pMiR-CIP2A(partial)

Plasmid

#53705

Purpose

Luciferase reporter assay for microRNA binding on CIP2A coding sequence

Depositor

Edward Chan

Article

Jung et al Mol Biol Cell. 2013 Jun;24(11):1638-48,

Insert

CIP2A (CIP2A Human)

Use

Luciferase

Available Since

Aug. 28, 2014

Availability

Academic Institutions and Nonprofits only