We narrowed to 4,125 results for: p2a

1. Crimson/P2A-ICP8 (pSLIK1)

   Plasmid

   #113856

   Purpose

   For Doxycycline-inducible expression of ICP8 synaptase, with red fluorescent protein gene E2-Crimson-P2A-ICP8

   Depositor

   Richard S Myers

   Article

   Valledor et al PLoS One. 2018 Aug 15;13(8):e0200955. do

   Insert

   ICP8
   

   Use

   Lentiviral

   Tags

   P2A/Crimson

   Expression

   Mutation

   Promoter

   Available since

   June 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. AttB_ACE2_IRES_iRFP670-H2A-P2A-PuroR

   Plasmid

   #190076

   Purpose

   express human ACE2 with selectable markers iRFP670 and puromycin resistance gene

   Depositor

   Kenneth Matreyek

   Article

   Roelle et al PLoS Biol. 2022 Jul 27;20(7):e3001738. d

   Insert

   ACE2 (ACE2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   none

   Available since

   Sept. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEMT-halo-HA-p2a-neoR

   Plasmid

   #161977

   Purpose

   homologous donor template for endogenous tagging

   Depositor

   Joanna Wysocka

   Article

   Gu et al Mol Cell. 2020 Nov 19;80(4):699-711.e7.

   Insert

   GAlinker-HA-halo-HA-p2a-neoR
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEMT-puroR-p2a-HA-halo

   Plasmid

   #161979

   Purpose

   homologous donor template for endogenous tagging

   Depositor

   Joanna Wysocka

   Article

   Gu et al Mol Cell. 2020 Nov 19;80(4):699-711.e7.

   Insert

   puroR-p2a-HA-halo-GSlinker
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. LV_EF1a_CDK4(R24C)-P2A-Hygro_Barcode

   Plasmid

   #170222

   Purpose

   Barcoded lentiviral vector to express CDK4 (R24C) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

   Depositor

   Prashant Mali

   Article

   Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

   Insert

   CDK4 (CDK4 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   R24C

   Promoter

   EF1a

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMY-MCS-P2A-PuroR-HA

   Plasmid

   #163352

   Purpose

   Retroviral backbone for in-frame cloning of a gene of interest and a P2A separated Puromycin selection marker

   Depositor

   Louis Ates, Teunis Geijtenbeek

   Article

   van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMY-MCS-P2A-CD90.1

   Plasmid

   #163354

   Purpose

   Retroviral backbone for in-frame cloning of a gene of interest and a P2A separated CD90.1 surface marker

   Depositor

   Louis Ates, Teunis Geijtenbeek

   Article

   van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. mKate2-P2A-APEX2-2xML1N(7Q)

   Plasmid

   #67666

   Purpose

   Mammalian expression vector driving APEX2-ML1N (Mouse 2xML1N domain (mutant version), [marks PI(3,5)P2] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.

   Depositor

   Rob Parton

   Article

   Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

   Insert

   mKate2-P2A-APEX2-ML1Nmut*2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLNCX2-mCherry-CHMP2A-siRNAres

   Plasmid

   #115332

   Purpose

   expresses siRNA-resistant mCherry-CHMP2A in mammalian cells

   Depositor

   Sanford Simon

   Article

   Johnson et al Elife. 2018 Jul 4;7. pii: 36221. doi: 10

   Insert

   CHMP2A (CHMP2A Human)
   

   Use

   Retroviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   mutated to be resistant to siRNA knockdown

   Promoter

   CMV

   Available since

   Sept. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. KP201: pMVP (L5-L4) eGFP-P2A

    Plasmid

    #121708

    Purpose

    pMVP L5-L4 entry plasmid, contains eGFP-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term eGFP linked by P2A to gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    eGFP-P2A (open)
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-SRGAP2A-FBAR-mRFP

    Plasmid

    #168502

    Purpose

    Expression of the F-BAR domain (aa1-501) of SRGAP2 with a C-terminal mRFP fusion.

    Depositor

    Franck Polleux

    Article

    Guerrier et al Cell. 2009 Sep 4;138(5):990-1004. doi: 1

    Insert

    SRGAP2A-FBAR (SRGAP2 Human)
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. KP301: pMVP (L5-L4) mCherry-P2A

    Plasmid

    #121710

    Purpose

    pMVP L5-L4 entry plasmid, contains mCherry-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term mCherry linked by P2A to gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    mCherry-P2A (open)
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. LV_EF1a_Caspase8(C360A)-P2A-Hygro_Barcode

    Plasmid

    #170217

    Purpose

    Barcoded lentiviral vector to express Caspase8 (C360A) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

    Depositor

    Prashant Mali

    Article

    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

    Insert

    CASP8 (CASP8 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    C360A

    Promoter

    EF1a

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-VQRRA-P2A-Puro

    Plasmid

    #110851

    Purpose

    Lentiviral vector for constitutive expression of Cas9-VQRRA in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-VQRRA
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    D1135V, R1335Q, T1337R and NLS sequence at the N-…

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKF-D2irTNP2AG-T2APuroR-5kwh

    Plasmid

    #128255

    Purpose

    Mammalian linearizer gene circuit based on negative feedback in a Flp-In expression system controlling the PuroR gene.

    Depositor

    Gabor Balazsi

    Article

    Farquhar et al Nat Commun. 2019 Jun 24;10(1):2766. doi:

    Insert

    hTetR::P2A::EGFP::T2A::PuroR
    

    Use

    Synthetic Biology; Flp-in expression vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-D2ir promoter

    Available since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. KP501: pMVP (L5-L4) eYFP-P2A

    Plasmid

    #121714

    Purpose

    pMVP L5-L4 entry plasmid, contains eYFP-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term eYFP linked by P2A to gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    eYFP-P2A (open)
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. LV_EF1a_ERalpha(Y537S)-P2A-Hygro_Barcode

    Plasmid

    #170225

    Purpose

    Barcoded lentiviral vector to express ERalpha (Y537S) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

    Depositor

    Prashant Mali

    Article

    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

    Insert

    ER (ESR1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    Y537S

    Promoter

    EF1a

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pME-nCas9n-P2A-mKate2-pA

    Plasmid

    #109547

    Purpose

    Multisite gateway vector for expression of nCas9n with bicistronic expression of mKate2

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    nCas9n and mKate2
    

    Use

    Zebrafish plasmids

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. MmCavin4-APEX2-P2A-mKate2

    Plasmid

    #170524

    Purpose

    Vector for expression of MmCavin4-APEX2-P2A-mKate2 in mammalian cells. Parton lab clone EJF

    Depositor

    Rob Parton

    Article

    Rae et al Elife. 2021 Apr 27;10. pii: 64630. doi:

    Insert

    Cavin4 (Cavin4 Mouse)
    

    Use

    Tags

    APEX2-P2A-mKate2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBS-M71-IRES-tauRFP2ACNf

    Plasmid

    #15510

    Depositor

    Peter Mombaerts

    Article

    Li et al Nature. 2004 Mar 25. 428(6981):393-9.

    Insert

    M71 targeting vector with IRES tauRFP-RFP ACN
    

    Use

    High copy number

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 24, 2007

    Availability

    Academic Institutions and Nonprofits only