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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 2,282 results for: sp1

Showing: 701 - 720 of 2282 results

  1. pRSFDuet-sumo-NSP12 (SARS-CoV-2)

    Plasmid
    #159107
    Purpose
    The SARS-CoV-2 NSP12 coding sequence was cloned into a modified pRSFDuet-1 vector (Novagen) bearing an N-terminal His6-SUMO-tag which is cleavable by the ubiquitin-like protease (ULP1).
    Depositor
    Thomas Tuschl
    Article
    Chen et al Cell. 2020 Sep 17;182(6):1560-1573.e13.
    Insert
    NSP12
    Use
    Tags
    His-SUMO tag
    Expression
    Bacterial
    Mutation
    None
    Promoter
    T7
    Available since
    Sept. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    159107-plasmid-map-sequence-id-310025

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  4. pDONR207 SARS-CoV-2 NSP1 Delta RC

    Plasmid
    #164522
    Purpose
    Gateway-compatible Entry vector, with insert of mutated NSP1 CDS from SARS-CoV-2 isolate Wuhan-Hu-1 (amino acid substitutions K164A/H165A)
    Depositor
    Mitchell Guttman
    Article
    Banerjee et al Cell. 2020 Nov 25;183(5):1325-1339.e21.
    Insert
    NSP1 Delta RC (ORF1ab )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Insert of NSP1 gene's CDS from SARS-CoV-2 is…
    Promoter
    Available since
    Feb. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    164522-plasmid-map-sequence-id-324313

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  6. pBIG1a_nsp12-His6-3xFlag (SARS-CoV-2)

    Plasmid
    #169182
    Purpose
    Baculoviral transfer vector to express nsp12-His6-3xFlag (SARS-CoV-2) in insect cells
    Depositor
    John Diffley
    Article
    Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443
    Insert
    nsp12-His6-3xFlag (ORF1ab SARS-CoV-2, Synthetic)
    Use
    Tags
    His6-3xFlag
    Expression
    Insect
    Mutation
    Codon optimised for insect cells (Spodoptera frug…
    Promoter
    Polyhedrin
    Available since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169182-plasmid-map-sequence-id-333999

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  9. EGFP in pDBEcR-pIND(SP1)/Bla

    Plasmid
    #201557
    Purpose
    Ecdysone-inducible vector with SB ITRs for expressing DBEcR (3xmyc- tagged) and FLAG- EGFP; blasticidin resistance.
    Depositor
    Randy Poon
    Article
    Yeung et al J Cell Sci. 2023 Apr 15;136(8):jcs260754
    Insert
    DBEcR and EGFP
    Use
    Unspecified; Sleeping beauty transposon
    Tags
    DBEcR-myc and FLAG-EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a and pIMD(SP1)
    Available since
    July 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    201557-plasmid-map-sequence-id-400936

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  10. EGFP in pDBEcR-pIND(SP1)-DD-W/Bla

    Plasmid
    #201560
    Purpose
    Ecdysone-inducible vector with SB ITRs for expressing FLAG- EGFP-DD (WPRE in 3' UTR) and DBEcR (3xmyc-tagged); blasticidin resistance.
    Depositor
    Randy Poon
    Article
    Yeung et al J Cell Sci. 2023 Apr 15;136(8):jcs260754
    Insert
    DBEcR and EGFP
    Use
    Unspecified; Sleeping beauty transposon
    Tags
    DBEcR-myc, FLAG-EGFP, and FLAG-EGFP-DD
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a and pIND(SP1)
    Available since
    July 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    201560-plasmid-map-sequence-id-400950

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  13. EGFP in pDBEcR-pIND(SP1)-DD/Bla

    Plasmid
    #201559
    Purpose
    Ecdysone-inducible vector with SB ITRs for expressing FLAG- EGFP-DD and DBEcR (3xmyc-tagged); blasticidin resistance.
    Depositor
    Randy Poon
    Article
    Yeung et al J Cell Sci. 2023 Apr 15;136(8):jcs260754
    Insert
    DBEcR and EGFP
    Use
    Unspecified; Sleeping beauty transposon
    Tags
    DBEcR-myc, FLAG-EGFP, and FLAG-EGFP-DD
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a and pIND(SP1)
    Available since
    July 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    201559-plasmid-map-sequence-id-400941

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  14. EGFP in pDBEcR-pIND(SP1)-W/Bla

    Plasmid
    #201558
    Purpose
    Ecdysone-inducible vector with SB ITRs for expressing FLAG- EGFP (WPRE in 3' UTR) and DBEcR (3xmyc-tagged); blasticidin resistance.
    Depositor
    Randy Poon
    Article
    Yeung et al J Cell Sci. 2023 Apr 15;136(8):jcs260754
    Insert
    DBEcR and EGFP
    Use
    Unspecified; Sleeping beauty transposon
    Tags
    DBEcR-myc and FLAG-EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a and pIND(SP1)
    Available since
    July 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    201558-plasmid-map-sequence-id-400924

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Showing: 701 - 720 of 2282 results