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pCheY Tc delta C

Plasmid

#13520

Depositor

James McKerrow

Publication

Eakin et al J Biol Chem. 1993 Mar 25. 268(9):6115-8.

Insert

cruzain

Tags

CheY

Expression

Bacterial

Mutation

Truncated variant: stop codon after Gly212

Available since

March 11, 2009

Availability

Academic Institutions and Nonprofits only
pYPQ230-RRV

Plasmid

#188543

Purpose

Gateway entry clone (attL1 & attR5) for LbCas12a-RRV mediated genome editing

Depositor

Yiping Qi

Publication

Zhang et al Genome Biol. 2023 Apr 30;24(1):102. doi:

Insert

LbCas12a-RRV

Use

CRISPR; Gateway compatible lbcas12a-rrv entry clo…

Tags

NLS

Expression

Plant

Mutation

G146R, D156R, R182V

Available since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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FLAG-3C-sGFP1-10_pcDNA3.1(-)

Plasmid

#182931

Purpose

For expression of membrane protein with N-terminal FLAG tag and C-terminal split GFP1-10 fragment

Depositor

William Weis

Publication

Bruguera et al J Biol Chem. 2022 Jan 21:101628. doi: 10

Type

Empty backbone

Tags

3C-cleavable split GFP 1-10, FLAG tag, and HA sig…

Expression

Mammalian

Promoter

CMV

Available since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pENTR L3-4XmCherry-HA-L2

Plasmid

#32315

Depositor

Steve Stowers

Publication

Petersen et al PLoS One. 2011;6(9):e24531. Epub 2011 Se

Insert

4XmCherry-HA

Use

Gateway multisite entry vector

Tags

HA

Available since

Sept. 15, 2011

Availability

Academic Institutions and Nonprofits only

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neurod1:CaMPARI

Plasmid

#173479

Purpose

CaMPARI is driven by -5kb neurod1 promoter.

Depositor

John Rawls

Publication

Ye et al Cell Host Microbe. 2021 Feb 10;29(2):179

Insert

CaMPARI

Use

Zebrafish expression

Available since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pUC19/ER-mChe

Plasmid

#183163

Purpose

Construct for transient expression of endoplasmic reticulum-targeted mCherry in plants.

Depositor

Yao-Guang Liu, Qinlong Zhu

Publication

Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

Insert

ER-mCherry

Tags

25 aa ER signal of Glycine soja kunitz-type tryps…

Expression

Plant

Available since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

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neurod1:Gal4; cmlc2:EGFP

Plasmid

#173478

Purpose

Gal4 is driven by -5kb neurod1 promoter. The vector contains a cmlc2:EGFP marker

Depositor

John Rawls

Publication

Ye et al Cell Host Microbe. 2021 Feb 10;29(2):179

Insert

Gal4

Use

Zebrafish expression

Available since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pmEOS2-VSVG

Plasmid

#98286

Purpose

mammalian expression of VSVG-mEOS2

Depositor

Mike Heilemann

Publication

Fricke et al Sci Rep. 2015 Sep 11;5:14072. doi: 10.10

Insert

VSVG

Tags

mEos2

Expression

Mammalian

Promoter

CMV

Available since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pT7-G-AsCas12a crRNA-BsaI cassette (MSP3491)

Plasmid

#114067

Purpose

T7 promoter crRNA entry vector used for in vitro transcription of AsCas12a guides

Depositor

Keith Joung, Benjamin Kleinstiver

Publication

Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

Insert

entry vector for AsCas12a crRNAs

Use

In vitro transcription

Available since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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p2T-CAG-SpCas9-BlastR

Plasmid

#107190

Purpose

Confers constitutive expression of SpCas9

Depositor

Richard Sherwood

Publication

Shen et al Nature. 2018 Nov;563(7733):646-651. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SpCas9 (NEWENTRY )

Use

CRISPR

Tags

3xFLAG

Expression

Bacterial and Mammalian

Promoter

Chicken ß-actin

Available since

Dec. 6, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pcDNA4/HisMaxB-YAP2-1st&2nd WW mutant

Plasmid

#19000

Depositor

Marius Sudol

Publication

Oka et al J Biol Chem. 2008 Oct 10;283(41):27534-2

Insert

Yes-kinase associated protein (YAP1 Human)

Tags

His

Expression

Mammalian

Mutation

Tryptophan 199 changed to Alanine, and Proline 2…

Available since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pENTR hH1-TERRA

Plasmid

#111381

Purpose

TERRA with approximately 130 telomere hexanucleotide repeats of TTAGGG and TTGGGG variant sequences under control of hH1 promoter and terminator

Depositor

Klaus Holzmann

Publication

Kreilmeier et al Genes (Basel). 2016 Aug 16;7(8). pii: ge

Insert

hH1-TERRA

Use

Gateway entry cloning

Promoter

hH1

Available since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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6his-WT gusA-pET28a+

Plasmid

#61156

Purpose

N -terminal His tagged wild-type GUS expression vector

Depositor

Andrew Ellington, Ichiro Matsumura

Publication

Matsumura et al Nat Biotechnol. 1999 Jul;17(7):696-701.

Insert

gusA

Tags

6x His

Expression

Bacterial

Promoter

T7

Available since

Jan. 8, 2015

Availability

Academic Institutions and Nonprofits only

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MAC-BSG

Plasmid

#158383

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Publication

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

BSG (BSG Human)

Tags

MAC-tag

Expression

Mammalian

Available since

Nov. 10, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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RBD-Fc

Plasmid

#164221

Purpose

Expresses soluble , dimeric Fc-His fusion protein for the original SARS-CoV2 Spike RBD region, along with dTomato reporter

Depositor

Sriram Neelamegham

Publication

Yang et al Elife. 2020 Oct 26;9. pii: 61552. doi: 1

Insert

Spike RBD (S SARS-CoV)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pRPaCas9

Plasmid

#111819

Purpose

Expresses Cas9 in T. brucei (2T1) cells

Depositor

David Horn

Publication

Rico et al Sci Rep. 2018 May 21;8(1):7960. doi: 10.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Cas9

Use

CRISPR

Available since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRK5 beta4 1355T

Plasmid

#16038

Depositor

Filippo Giancotti

Publication

Nikolopoulos et al Mol Cell Biol. 2005 Jul . 25(14):6090-10

Insert

beta4 (ITGB4 Human)

Expression

Mammalian

Mutation

1355T: amino acids 1-1355

Available since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pRSF-G1(4/5FTrp)RS

Plasmid

#207620

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of 4-Fluoro-L-Tryptophan (4FTrp) and 5-Fluoro-L-Tryptophan (5FTrp) into proteins in E. coli in response to the amber (TAG) codon

Depositor

Thomas Huber

Publication

Qianzhu et al J Am Chem Soc. 2024 Apr 30. doi: 10.1021

Insert

tRNA synthetase

Expression

Bacterial

Promoter

T7

Available since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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pBES1-LacOx50-Hyg

Plasmid

#210261

Purpose

Integrates 50xLacO repeats upstream of the BES1 promoter with a hygromycin drug selection marker downstream of the promoter.

Depositor

Calvin Tiengwe

Publication

Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

Insert

50xLacO repeats derived from: PMID: 12864855.

Available since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

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TB201

Bacterial strain

#230031

Purpose

Fluorescently labelled (YFP) E. coli

Depositor

Tobias Bergmiller, Calin Guet

Publication

Tomasek et al J Biotechnol. 2018 Feb 20;268:40-52. doi

Bacterial resistance

None

Available since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only