We narrowed to 22,830 results for: Sis

1. pMax_mOrange_A215C

   Plasmid

   #177828

   Purpose

   Site-specific mutagenesis of mOrange for the purposes of creating a site-specific 8-oxo-G:C lesion for the purposes of measuring OGG1 activity via Host Cell Reactivation (HCR).

   Depositor

   Leona Samson, Zachary Nagel

   Article

   Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

   Insert

   mOrange_A215C
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   A215C

   Promoter

   CMV

   Available Since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMBEC2

   Plasmid

   #187596

   Purpose

   Base editing plasmid, constitutively expressing cytosine base editor and cas6. Contains GFP, flanked by BsaI restriction sites to introduce spacer and gRNAs. Kanamycin resistance

   Depositor

   Pablo Ivan Nikel

   Article

   Volke et al Nat Commun. 2022 May 31;13(1):3026. doi:

   Insert

   nCas9-BEC-UGI; cas6f, gfp
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   trc

   Available Since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. NPY-pHTomato

   Plasmid

   #83501

   Purpose

   Expression of a fluorescent granule marker, neuropeptide-Y, for localization and exocytosis studies.

   Depositor

   Sebastian Barg

   Article

   Gandasi et al PLoS One. 2015 Jun 19;10(6):e0127801. do

   Insert

   NPY (NPY Human)
   

   Use

   Tags

   pHTomato

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available Since

   Nov. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. hHSF1 K80Q

   Plasmid

   #118351

   Purpose

   Plasmid expresses hHSF1 with K80 in DBD mutated to Q, which abolish DNA-binding activity of HSF1.

   Depositor

   Lea Sistonen

   Article

   Westerheide et al Science. 2009 Feb 20. 323(5917):1063-6.

   Insert

   hHSF1 (HSF1 Human)
   

   Use

   Tags

   myc and 6xHIS

   Expression

   Mammalian

   Mutation

   K80Q

   Promoter

   Available Since

   Nov. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pQE-BR1

   Plasmid

   #160333

   Purpose

   This plasmid encodes an engineered oxygen-independent flavin binding fluorescent protein with N-terminal 6xHis tag. Its quantum yield is improved compared to wild type.

   Depositor

   Sang Taek Jung

   Article

   Ko et al J Agric Food Chem. 2019 Oct 30;67(43):12

   Insert

   BR1
   

   Use

   Tags

   6xHis tag

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available Since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMax_GFP_C289T

   Plasmid

   #177826

   Purpose

   Site-specific mutagenesis of GFP for the purposes of creating a site-specific hypoxanthine lesion for the purposes of measuring MPG activity via Host Cell Reactivation (HCR).

   Depositor

   Leona Samson, Zachary Nagel

   Article

   Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

   Insert

   GFP_C289T
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   C289T

   Promoter

   CMV

   Available Since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHLuc (Antares-SEPLuc C1A4E+6)

   Plasmid

   #141089

   Purpose

   final optimized bioluminescent reporter for detecting extracellular pH changes in tumor acidosis

   Depositor

   Jeak Ling Ding

   Article

   Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

   Insert

   Antares-T2A-puromycin-IRESv24-SEP-C1A4E+6
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDFDuet-AtUBC8-S-AtUBA1-S

   Plasmid

   #222714

   Purpose

   Expression AtUBA1-S and AtUBC8-S in in bacterial cells

   Depositor

   Lei Wu

   Article

   Pu et al Biomolecules. 2024 Sep 25;14(10):1209. d

   Inserts

   AtUBC8 (UBC8 Mustard Weed)
   ATUBA1 (UBA1 Mustard Weed)
   

   Use

   Tags

   S-Tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Nov. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. mRFP-mem

   Plasmid

   #55622

   Purpose

   This red fluorescent plasma membrane marker consists of the first twenty residues of neuromodulin fused to mRFP.

   Depositor

   Catherine Berlot

   Article

   Mervine et al Mol Pharmacol. 2006 Jul;70(1):194-205. E

   Insert

   mRFP-Mem
   

   Use

   Tags

   The N-terminal 20 residues of neuromodulin are fu…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pICE-NLS-mCherry

    Plasmid

    #60364

    Purpose

    Plasmid for expression of NLS-mCherry (control plasmid). Confers resistance to Puromycin. Expression is inducible in T-REx cells.

    Depositor

    Sébastien Britton, Patrick Calsou

    Article

    Britton et al Nucleic Acids Res. 2014 Oct 1;42(14):904

    Insert

    NLS-mCherry
    

    Use

    Tags

    NLS from SV40 T antigen and mCherry

    Expression

    Mutation

    Promoter

    CMV-tet

    Available Since

    Jan. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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