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Plasmid#56557PurposeLocalization: Endoplasmic Reticulum, Excitation: 516, Emission: 529DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertER (CALR Human)
TagsmCitrineExpressionMammalianMutationER Signal PeptidePromoterCMVAvailable sinceDec. 5, 2014AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
mKO2-LaminB1-10
Plasmid#57878PurposeLocalization: Nuclear Envelope, Excitation: 551, Emission: 565DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLaminB1 (LMNB1 Human)
TagsmKO2ExpressionMammalianPromoterCMVAvailable sinceOct. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-ATG5-N-14
Plasmid#54001PurposeLocalization: Membrane Receptor, Excitation: 487, Emission: 509DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertATG5 (ATG5 Human)
TagsmEmeraldExpressionMammalianPromoterCMVAvailable sinceJune 16, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHPHS232
Plasmid#228356PurposeLanding pad with Bxb1 attP_wildtype site, Dox-inducible BFP-iCasp9-Blasticidin, CMV-driven rtTA3, T2A-neomycin for HDR into AAVS1 locusDepositorInsertsmTagBFP2
Reverse tetracycline transactivator 3
neo
TagsBlasticidin S deaminase and iCasp9ExpressionMammalianPromoterTRE3GV and gene trapAvailable sinceJan. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-Synaptojanin-1-145
Plasmid#223563PurposeExpresses the short isoform of SYNJ1 (neuronal) with mCherry tagged at the n-terminusDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSYNJ1 (SYNJ1 Human)
TagsHis and mCherryExpressionMammalianAvailable sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
CCR7-DuET
Plasmid#213203PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-DRD2-cpHalo∆-mEGFP
Plasmid#194679PurposeCMV driven expression of D2R GPCR recorder in mammalian cell lines fused to mEGFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertDRD2_cpHalo∆
TagsmEGFPExpressionMammalianPromoterCMVAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
VGAT scFv [L118/14]
Plasmid#206754PurposeMammalian Expression of VGAT scFV. Derived from hybridoma L118/14 scFv.DepositorAvailable sinceJan. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-GRAB_NTS1.0
Plasmid#208670PurposeExpresses the genetically-encoded fluorescent neurotensin (NTS) sensor GRAB_NTS1.0 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGPCR activation based neurotensin (NTS) sensor GRAB_NTS1.0
ExpressionMammalianPromoterCMVAvailable sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG1345 mCherry-FIREtag-DEVDG-MoA
Plasmid#205176PurposeExpresses mCherry-FIREtag-DEVDG-MoA in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmCherry-cagedFIREtag
ExpressionMammalianAvailable sinceSept. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSFFV_Gal4LOV[SK22]-VP64-IRES-mCherry (pLZA140)
Plasmid#203907PurposeEncodes LightsOut (AsLOV2(408-543) inserted in Gal4 SK22) transcription factor in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGal4LOV(SK22)-VP64-IRES-mCherry
ExpressionMammalianPromoterSFFVAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV-GfaABC1D-iCreV-4x6T
Plasmid#196412PurposeAstrocytic expression of light-inducible iCreV recombinase in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassetteDepositorInsertiCreV
UseAAV; Astrocyte-selectiveExpressionMammalianPromoterCAG and GfaABC1DAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-SA-neo-CAGGS-PE2-2A-GFP
Plasmid#180014PurposeTargeting vector for knocking in constitutively expressed PE2-2A-GFP into the human AAVS1 locusDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPE2-2A-GFP
UseCRISPR; Human genome engineeringTagsGFPPromoterCAGAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
tdTomato-LacI
Plasmid#183921PurposeLac repressor labeled with tdTomato; can be used to label lacO sitesDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserttdTomato-LacI (lacI Synthetic)
ExpressionMammalianMutationnonePromoterCMVAvailable sinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1a-HygroR-T2A-TetOn3G
Plasmid#179887PurposeExpresses TetOn3G transcription factor selectable by hygromycin resistance (Lentiviral vector)DepositorInsertTetOn3G
UseLentiviralTagsHygromycin resistanceExpressionBacterial and MammalianPromoterEF1aAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCS2-mCherry-Rab11a (S25N)
Plasmid#184036PurposeExpresses dominate negative mCherry Rab11a in mammalian cells and zebrafish embryosDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRab11
TagsmCherryExpressionMammalianMutationSerine 25 changed to AsparaginePromoterCMV IE94Available sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
ND4.2-Left TALE-G1397-N-dead-mCherry
Plasmid#179683PurposeExpression of base editor in mammalian cells and for cell sortingDepositorInsertCOX8a MTS_3xFLAG_ND4.2 Left TALE_2aa_DddA G1397-N (E1347A)_1xUGI_P2A_mCherry_ATP5B 3'UTR
ExpressionMammalianMutationE1347AAvailable sinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSmart-EF1a-Nla-cas5
Plasmid#178878PurposeExpression of human codon-optimized Nlacas5 with C terminal NLS and HA tagDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEF1a-NlaCas5c-bGH polyA
TagsHA and NLSExpressionMammalianPromoterEF1aAvailable sinceJan. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSmart-EF1a-Nla-cas7
Plasmid#178879PurposeExpression of human codon-optimized Nlacas7 with C terminal NLS and HA tagDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEF1a-NlaCas7c-bGH PolyA
TagsHA and NLSExpressionMammalianPromoterEF1aAvailable sinceJan. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSmart-EF1a-Nla-cas8
Plasmid#178880PurposeExpression of human codon-optimized NlaCas8 with N terminal NLS and HA tagDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEF1a-NlaCas8c-bGH PolyA
TagsHA and NLSExpressionMammalianPromoterEF1aAvailable sinceJan. 18, 2022AvailabilityAcademic Institutions and Nonprofits only