We narrowed to 59,942 results for: %s

1. pAAV-EF1a-iDreV (AAV PHP.eB)

   Viral Prep

   #140136-PHPeB

   Purpose

   Ready-to-use AAV PHP.eB particles produced from pAAV-EF1a-iDreV (#140136). In addition to the viral particles, you will also receive purified pAAV-EF1a-iDreV plasmid DNA. EF1a-driven, light-inducible iDreV recombinase for spatiotemporally precise optogenomic modifications. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Hongkui Zeng

   Article

   Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s

   Promoter

   EF1a

   Available Since

   Nov. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

2. pCMV4 p50

   Plasmid

   #21965

   Depositor

   Warner Greene

   Article

   Ballard et al Proc Natl Acad Sci U S A. 1992 Mar 1. 89

   Insert

   NF-kB p50 subunit (NFKB1 Human)
   

   Expression

   Mammalian

   Available Since

   Sept. 17, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCH1/RAD51o

   Plasmid

   #102562

   Purpose

   Express Human RAD51 protein codon optimzed for E.coli and the E.coli GorE Operon

   Depositor

   Maria Spies

   Article

   Subramanyam et al Proc Natl Acad Sci U S A. 2016 Oct 11;11

   Inserts

   Homo sapiens RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae) (RAD51), transcript variant 1 (RAD51 Synthetic, Human)
   E. coli groE operon
   

   Expression

   Bacterial

   Mutation

   Codon optimized for E.coli Expression

   Promoter

   T7

   Available Since

   Feb. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. GFP-Intersectin Short

   Plasmid

   #47394

   Depositor

   Peter McPherson

   Article

   Hussain et al Nat Cell Biol. 2001 Oct;3(10):927-32.

   Insert

   Intersectin short (ITSN1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. TadA-NG-CBE

   Plasmid

   #227420

   Purpose

   C-to-T base editing in mammalian cells

   Depositor

   Yang Sun

   Article

   Liu et al Adv Sci (Weinh). 2024 Sep 19:e2405628. d

   Insert

   TadA-nSpCas9-NG-2xUGI (AICDA Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. AID-NG-CBE

   Plasmid

   #227416

   Purpose

   C-to-T base editing in mammalian cells

   Depositor

   Yang Sun

   Article

   Liu et al Adv Sci (Weinh). 2024 Sep 19:e2405628. d

   Insert

   AID-nSpCas9-NG-2xUGI (AICDA Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. Flag Intersectin Short (human)

   Plasmid

   #47392

   Depositor

   Peter McPherson

   Article

   Hussain et al Nat Cell Biol. 2001 Oct;3(10):927-32.

   Insert

   Intersectin short (ITSN1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 29, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pACEBac1-PSMA5-PSMA6-PSMA7

   Plasmid

   #217894

   Purpose

   Insect cell expression of human 20S CP proteasome subunit alpha type-5 (PSMA5), alpha type-6 (PSMA6), and alpha type-7 (PSMA7)

   Depositor

   Brenda Schulman

   Article

   Adolf et al Nat Struct Mol Biol. 2024 Apr 10. doi: 1

   Inserts

   PSMA5 (PSMA5 Human)
   PSMA6 (PSMA6 Human)
   PSMA7 (PSMA7 Human)
   

   Expression

   Insect

   Available Since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSin-5HT-1.0

   Plasmid

   #172882

   Purpose

   make sindbis virus of 5-HT sensor GRAB_5-HT1.0 and express in cells

   Depositor

   J. Julius Zhu

   Article

   Wan et al Nat Neurosci. 2021 Apr 5. pii: 10.1038/s

   Insert

   5HT SENSOR
   

   Expression

   Mammalian

   Promoter

   sp6

   Available Since

   Oct. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAS18

    Plasmid

    #158787

    Purpose

    Ribosome-tagged GCaMP (soma localized) expression pan-neuronally C. elegans

    Depositor

    Zachary Knight, Jennifer Garrison

    Article

    Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

    Insert

    Ptag-168::GCaMP6m::rpl-1a
    

    Expression

    Worm

    Available Since

    Sept. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUDP012

    Plasmid

    #101167

    Purpose

    E. coli/S. cerevisiae shuttle vector carrying amdS andSpcas9D147Y P411T and expressing a ribozyme flanked g-RNA for Cas9 editing targeting the gene SeILV6 and in S. pastorianus (HH-gRNASeILV6-HDV)

    Depositor

    Jean-Marc Daran

    Article

    de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.

    Insert

    HH-gRNA-HDV targetting SeILV6 in S. pastorianus
    

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    ScTDH3

    Available Since

    Dec. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGL3-U6-pegRNA_KCNA1-EGFP

    Plasmid

    #159787

    Purpose

    S. pyogenes pegRNA for C to A mutation at the KCNA1 site of human cells using prime editing

    Depositor

    Xiaolong Wang

    Article

    Liu et al Cell Discov. 2020 Apr 28;6:27. doi: 10.1

    Insert

    spacer of pegRNA targeting KCNA1 gene (KCNA1 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGL3-U6-sgRNA_KCNA1-mcherry

    Plasmid

    #159788

    Purpose

    S. pyogenes sgRNA collocated with pegRNA targeting human KCNA1 gene

    Depositor

    Xiaolong Wang

    Article

    Liu et al Cell Discov. 2020 Apr 28;6:27. doi: 10.1

    Insert

    spacer of sgRNA targeting KCNA1 gene (KCNA1 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJS699

    Plasmid

    #168779

    Purpose

    Encodes the SARS-CoV-2 Spike Receptor Binding Domain (RBD) and can be combined with pJS697 through homologous recombination

    Depositor

    Timothy Whitehead

    Article

    Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:

    Insert

    Spike RBD (S betacoronavirus)
    

    Tags

    Cmyc

    Mutation

    N343Q

    Promoter

    GAL1

    Available Since

    Aug. 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. DJ-1 TEV site pET15b

    Plasmid

    #60687

    Purpose

    Recombinant protein expression of Park7

    Depositor

    Mark Wilson

    Article

    Milkovic et al Protein Sci. 2015 Oct;24(10):1671-85. do

    Insert

    DJ-1 (PARK7 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    TEV site was inserted (see comments below).

    Promoter

    T7

    Available Since

    Oct. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pGL3-U6-pegRNA_FANCF-EGFP

    Plasmid

    #159785

    Purpose

    S. pyogenes pegRNA for C to A mutation at the FANCF site of human cells using prime editing

    Depositor

    Xiaolong Wang

    Article

    Liu et al Cell Discov. 2020 Apr 28;6:27. doi: 10.1

    Insert

    spacer of pegRNA targeting FANCF gene (FANCF Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGL3-U6-pegRNA_HOXD13_CtoT-EGFP

    Plasmid

    #159792

    Purpose

    S. pyogenes pegRNA for C to T mutation at the HOXD13 site of mouse cells using prime editing

    Depositor

    Xiaolong Wang

    Article

    Liu et al Cell Discov. 2020 Apr 28;6:27. doi: 10.1

    Insert

    spacer of pegRNA_HOXD13_CtoT targeting HOXD13 gene (HOXD13 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGL3-U6-pegRNA_HOXD13_CtoG-EGFP

    Plasmid

    #159791

    Purpose

    S. pyogenes pegRNA for C to G mutation at the HOXD13 site of mouse cells using prime editing

    Depositor

    Xiaolong Wang

    Article

    Liu et al Cell Discov. 2020 Apr 28;6:27. doi: 10.1

    Insert

    spacer of pegRNA_HOXD13_CtoG targeting HOXD13 gene (HOXD13 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAS19

    Plasmid

    #158788

    Purpose

    Ribosome-tagged GCaMP (soma localized) expression in C. elegans ASH neurons

    Depositor

    Zachary Knight, Jennifer Garrison

    Article

    Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

    Insert

    psra-6::GCaMP6m::rpl-1a
    

    Expression

    Worm

    Available Since

    Sept. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAS16

    Plasmid

    #158786

    Purpose

    Ribosome-tagged GCaMP (soma localized) expression in C. elegans AFD neurons (and others)

    Depositor

    Zachary Knight, Jennifer Garrison

    Article

    Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

    Insert

    Pntc-1::GCaMP6m::rpl-1a
    

    Expression

    Worm

    Available Since

    Sept. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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