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pCFD3-frame_selector_1

Plasmid

#127554

Purpose

Drosophila expression of frame selector sgRNA 1 that targets CRISPaint site for homology-independent knock-in

Depositor

Norbert Perrimon

Article

Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

Insert

sgRNA frame 1

Use

CRISPR

Expression

Insect

Promoter

dU6:3

Available Since

July 16, 2019

Availability

Academic Institutions and Nonprofits only

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OA-1053A

Plasmid

#184006

Purpose

Expresses arrays of gRNA targeting eve promoter under U6b/c promoter

Depositor

Omar Akbari

Article

Bui et al PLoS Pathog. 2023 Jan 19;19(1):e1010842.

Insert

U6b:sgRNAeve1 - U6c:sgRNAeve2

Expression

Insect

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCFD3-frame_selector_2

Plasmid

#127555

Purpose

Drosophila expression of frame selector sgRNA 2 that targets CRISPaint site for homology-independent knock-in

Depositor

Norbert Perrimon

Article

Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

Insert

sgRNA frame 2

Use

CRISPR

Expression

Insect

Promoter

dU6:3

Available Since

July 16, 2019

Availability

Academic Institutions and Nonprofits only

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pMH5

Plasmid

#52531

Purpose

expresses FLP recombinase in Drosophila

Depositor

Klaus Foerstemann

Article

Bottcher et al Nucleic Acids Res. 2014 Apr 19.

Insert

FLP recombinase (flp Budding Yeast)

Expression

Insect

Promoter

ubiquitin

Available Since

April 23, 2014

Availability

Academic Institutions and Nonprofits only

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pUb dPspCas13b + PspCas13b guide RNA

Plasmid

#176306

Purpose

Expresses catalytic dead PspCas13b and its associated guide RNA in Drosophila cells

Depositor

Jeremy Wilusz

Article

Ai et al Nucleic Acids Res. 2022 Mar 4. pii: 6542

Insert

PspCas13b

Tags

HA tag

Expression

Insect

Mutation

H133A, H1058A

Promoter

Ubi-p63e

Available Since

March 7, 2022

Availability

Academic Institutions and Nonprofits only

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U6b

Plasmid

#117222

Purpose

Expresses gRNA in Aedes aegypti

Depositor

Omar Akbari

Article

Li et al Elife. 2020 Jan 21;9. pii: 51701. doi: 1

Insert

HR U6b promoter-gRNA

Use

Synthetic Biology

Expression

Insect

Promoter

U6

Available Since

Jan. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pGL3-Gateway-DSCP

Plasmid

#71506

Purpose

flySTARR-seq validation vector with Gateway cassette upstream of the firefly luciferase gene and the DSCP core promoter

Depositor

Alexander Stark

Article

Arnold et al Science. 2013 Mar 1;339(6123):1074-7. do

Type

Empty backbone

Use

Flystarr-seq validation vector

Tags

luciferase

Expression

Insect

Available Since

Dec. 29, 2015

Availability

Academic Institutions and Nonprofits only

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pUASp YFP Rab5 WT

Plasmid

#53468

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab05 (Rab5 Fly)

Tags

YFP

Expression

Insect

Promoter

GAL4

Available Since

June 10, 2014

Availability

Academic Institutions and Nonprofits only
pENTR R4 ATCC LacZ GCTT

Plasmid

#173668

Purpose

Destination vector for Golden Gate Cloning assemblies, blue-white screening

Depositor

Eric Marois

Article

Green et al Elife. 2023 Dec 5;12:e93142. doi: 10.755

Type

Empty backbone

Expression

Bacterial and Insect

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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EK0211 CMV-TO-BFPmut-stop-t17d9b (FLP-IN)

Plasmid

#191139

Purpose

Expression of Trigger 2 in 3' UTR of an inactive BFP in mammalian cells. Used as second input in OR, AND gates

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

t17d9b

Expression

Mammalian

Mutation

WT

Promoter

CMV-TO

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pBiex-1 Myosin VI HMM_SNAP

Plasmid

#82716

Purpose

Expresses Myosin VI HMM with a C-terminal SNAP tag.

Depositor

Sivaraj Sivaramakrishnan

Article

Hariadi et al Nat Nanotechnol. 2015 Aug;10(8):696-700.

Insert

Myosin VI

Tags

6xHis Tag, FLAG purification tag, and SNAP tag

Expression

Bacterial and Insect

Promoter

T7 Promoter

Available Since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pKLBac-FluB-trimer

Plasmid

#229859

Purpose

Influenza B/Memphis/13/03 polymerase heterotrimer (self-cleaving polyprotein)

Depositor

Stephen Cusack

Article

Reich et al Nature. 2014 Dec 18;516(7531):361-6. doi

Inserts

PA, Influenza B (B/Memphis/13/03)
PB1, Influenza B (B/Memphis/13/03)
PB2, Influenza B (B/Los Angeles/1/02)

Tags

2x-Streptag and 8xHis-tag

Expression

Insect

Available Since

Feb. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pGEM-3XP3.RFP-tan

Plasmid

#83474

Purpose

RFP selectable marker for use in two-stage CRISPR-mediated allele replacement strategies in Drosophila. D. americana tan homology arms can be replaced with locus of choice.

Depositor

Patricia Wittkopp

Article

Lamb et al Fly (Austin). 2016 Aug 5:1-12.

Insert

3XP3.RFP-tan

Use

CRISPR

Expression

Bacterial and Insect

Promoter

3XP3 eye-expression Promoter

Available Since

Oct. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pJFRC-20X-UAS-ADARcd

Plasmid

#81173

Purpose

identification of cell-specific targets of RNA binding proteins

Depositor

Michael Rosbash

Article

McMahon et al Cell. 2016 Mar 30. pii: S0092-8674(16)30

Insert

UAS-ADARcd

Expression

Insect

Promoter

UAS

Available Since

Aug. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pHACK(Gal4)-DONR(T2A-Cas9)

Plasmid

#194768

Purpose

To insert T2A-Cas9 into Gal4 coding sequence in Drosophila, converting Gal4 into a T2A-Cas9 transgene.

Depositor

Chun Han

Article

Koreman et al Proc Natl Acad Sci U S A. 2021 Apr 6;118

Insert

T2A-Cas9

Use

CRISPR

Expression

Insect

Available Since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pBac{3XP3::EGFP, Pactin::Ptrsps}

Plasmid

#86861

Purpose

Expresses EGFP in eyes and ubiquitous expression of P-transposase

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2017 Apr 3;7(4):1339-1347

Insert

pACNNTNPII-S129A

Expression

Insect

Available Since

June 9, 2017

Availability

Academic Institutions and Nonprofits only

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pAC-CR7T-gRNA2.1-nlsBFP

Plasmid

#170515

Purpose

gRNA cloning vector containing a CR7T promoter, gRNA(2.1) scaffold, and a Ubi-mTagBFP-NLS marker; optimized for germline CRISPR.

Depositor

Chun Han

Article

Koreman et al Proc Natl Acad Sci U S A. 2021 Apr 6;118

Type

Empty backbone

Use

CRISPR

Expression

Insect

Promoter

CR7T

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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phage UbiC SNAP-KDEL

Plasmid

#104982

Purpose

Lentiviral vector expressing SNAP-KDEL for ER labeling

Depositor

Jeffrey Chao

Article

Voigt et al Cell Rep. 2017 Dec 26;21(13):3740-3753.

Insert

SNAP-KDEL

Use

Lentiviral

Tags

Secretion signal

Expression

Bacterial and Mammalian

Promoter

UbiC

Available Since

Feb. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pPath-pTac-integrase

Plasmid

#199999

Purpose

Entry vector for biosynthetic pathway. pTac-phiC31 integrase. This material contains 2 plasmids. For more information look under the "Depositor Comments" section below.

Depositor

Farren Isaacs

Article

Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

Insert

Pathways

Expression

Bacterial

Available Since

Feb. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pTriEx-mCherry-LOVSD

Plasmid

#81033

Purpose

Mammalian expression of the super-dark state of LOV2

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

LOVSD

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Mutation

C450A, L514K, G528A, L531E, N538E

Available Since

Aug. 17, 2016

Availability

Academic Institutions and Nonprofits only

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