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pHR-UCOE-EF1a-Zim3-dCas9-P2A-BFP

Plasmid

#188777

Purpose

dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

Depositor

Marco Jost, Jonathan Weissman

Publication

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

Zim3-dCas9

Use

Lentiviral

Tags

HA-2xNLS and Zim3 KRAB-NLS fusion

Promoter

EF1a

Available since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pHR-UCOE-EF1a-Zim3-dCas9-P2A-BFP

Plasmid

#188777

Purpose

dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

Depositor

Marco Jost, Jonathan Weissman

Publication

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

Zim3-dCas9

Use

Lentiviral

Tags

HA-2xNLS and Zim3 KRAB-NLS fusion

Promoter

EF1a

Available since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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hSyn-DIO-somBiPOLES-mCerulean (AAV9)

Viral prep

#154951-AAV9

Purpose

Ready-to-use AAV9 particles produced from hSyn-DIO-somBiPOLES-mCerulean (#154951). In addition to the viral particles, you will also receive purified hSyn-DIO-somBiPOLES-mCerulean plasmid DNA. Synapsin-driven, Cre-dependent expression of soma-targeted BiPOLES for optogenetic inhibition (blue light) and activation (red light). These AAV preparations are suitable purity for injection into animals.

Depositor

Simon Wiegert

Publication

Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

Promoter

Syn

Tags

mCerulean (Cre-dependent)

Available since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only
pLentiRNAGuide_002 - hU6-RfxCas13d-DR-BsmBI-EFS-Puro-WPRE

Plasmid

#138151

Purpose

For cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

Depositor

Neville Sanjana

Publication

Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

Insert

hU6_Rfxcas13d-DR_BsmBI

Use

Lentiviral

Available since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pLentiRNAGuide_002 - hU6-RfxCas13d-DR-BsmBI-EFS-Puro-WPRE

Plasmid

#138151

Purpose

For cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

Depositor

Neville Sanjana

Publication

Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

Insert

hU6_Rfxcas13d-DR_BsmBI

Use

Lentiviral

Available since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pAAV-hSyn-GRAB_DA1h (AAV9)

Viral prep

#113050-AAV9

Purpose

Ready-to-use AAV9 particles produced from pAAV-hSyn-GRAB_DA1h (#113050). In addition to the viral particles, you will also receive purified pAAV-hSyn-GRAB_DA1h plasmid DNA. Synapsin-driven expression of GRAB-DA1h dopamine sensor in neurons. These AAV preparations are suitable purity for injection into animals.

Depositor

Yulong Li

Publication

Sun et al Cell. 2018 Jul 12;174(2):481-496.e19. do

Promoter

Syn

Available since

Nov. 19, 2019

Availability

Academic Institutions and Nonprofits only
pAAV-DJ-N589X

Plasmid

#130878

Purpose

Encodes the AAV-DJ capsid with the N589 residue replaced with a TAG stop codon

Depositor

Prashant Mali

Publication

Katrekar et al Sci Rep. 2018 Feb 26;8(1):3589. doi: 10.

Insert

AAV-DJ cap N589X

Use

AAV

Expression

Mammalian

Mutation

N589X

Promoter

Unknown

Available since

Sept. 3, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pAAV-DJ-N589X

Plasmid

#130878

Purpose

Encodes the AAV-DJ capsid with the N589 residue replaced with a TAG stop codon

Depositor

Prashant Mali

Publication

Katrekar et al Sci Rep. 2018 Feb 26;8(1):3589. doi: 10.

Insert

AAV-DJ cap N589X

Use

AAV

Expression

Mammalian

Mutation

N589X

Promoter

Unknown

Available since

Sept. 3, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pET23b-15F11-HA-mEGFP-6xHis

Plasmid

#129593

Purpose

Encodes anti-HA scFv (anti-HA frankenbody) fused with mEGFP and His-tag. This plasmid is for bacteria protein expression under T7 promoter and protein purification with HisTrap.

Depositor

Tim Stasevich

Publication

Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:

Insert

Anti-HA frankenbody-mEGFP (15F11-HA scFv-mEGFP)

Tags

6xHis-tag

Expression

Bacterial

Promoter

T7

Available since

Aug. 15, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

2 Articles

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pET23b-15F11-HA-mEGFP-6xHis

Plasmid

#129593

Purpose

Encodes anti-HA scFv (anti-HA frankenbody) fused with mEGFP and His-tag. This plasmid is for bacteria protein expression under T7 promoter and protein purification with HisTrap.

Depositor

Tim Stasevich

Publication

Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:

Insert

Anti-HA frankenbody-mEGFP (15F11-HA scFv-mEGFP)

Tags

6xHis-tag

Expression

Bacterial

Promoter

T7

Available since

Aug. 15, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

2 Articles

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PyronicSF/pcDNA3.1(-)

Plasmid

#124812

Purpose

Highly resposive GFP-based pyruvate nanosensor.

Depositor

Luis Felipe Barros

Publication

Arce-Molina et al Elife. 2020 Mar 6;9. pii: 53917. doi: 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PyronicSF

Expression

Mammalian

Promoter

CMV

Available since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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PyronicSF/pcDNA3.1(-)

Plasmid

#124812

Purpose

Highly resposive GFP-based pyruvate nanosensor.

Depositor

Luis Felipe Barros

Publication

Arce-Molina et al Elife. 2020 Mar 6;9. pii: 53917. doi: 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PyronicSF

Expression

Mammalian

Promoter

CMV

Available since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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PLJR965

Plasmid

#115163

Purpose

Sth1 dCas9 TetR and KanR L5 Int attP for M. tuberculosis

Depositor

Sarah Fortune

Publication

Rock et al Nat Microbiol. 2017 Feb 6;2:16274. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

Sth1 sgRNA scaffold
Sth1 dCas9
TetR (deleted TetON) MtbCO
L5 attP
L5 Int
KanR

Use

Unspecified

Available since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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PLJR965

Plasmid

#115163

Purpose

Sth1 dCas9 TetR and KanR L5 Int attP for M. tuberculosis

Depositor

Sarah Fortune

Publication

Rock et al Nat Microbiol. 2017 Feb 6;2:16274. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

Sth1 sgRNA scaffold
Sth1 dCas9
TetR (deleted TetON) MtbCO
L5 attP
L5 Int
KanR

Use

Unspecified

Available since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pmCherry-C1-mTrim21

Plasmid

#105516

Purpose

Expression of m-Cherry-tagged wildtype mouse Trim21 in mammalian cells

Depositor

Melina Schuh

Publication

Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TRIM21 (Trim21 Mouse)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pmCherry-C1-mTrim21

Plasmid

#105516

Purpose

Expression of m-Cherry-tagged wildtype mouse Trim21 in mammalian cells

Depositor

Melina Schuh

Publication

Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TRIM21 (Trim21 Mouse)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCAG-D1R

Plasmid

#89160

Purpose

Express vaccinia virus D1R in mammalian cells

Depositor

Takeshi Kobayashi

Publication

Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Vaccinia virus D1R

Expression

Mammalian

Promoter

CAG promotor

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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pCAG-D1R

Plasmid

#89160

Purpose

Express vaccinia virus D1R in mammalian cells

Depositor

Takeshi Kobayashi

Publication

Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Vaccinia virus D1R

Expression

Mammalian

Promoter

CAG promotor

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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pUbC-OsTIR1-myc-IRES-scFv-sfGFP

Plasmid

#84563

Purpose

Bicistronic vector for expression of O. sativa E3 ubiquitin ligase and single chain variable fragment of GCN4 antibody fused to superfolder GFP

Depositor

Robert Singer

Publication

Wu et al Science. 2016 Jun 17;352(6292):1430-5. d

Insert

Transport inhibitor response 1 (E3 ligase domain), IRES, single chain variable fragment-superfolder GFP

Use

Lentiviral

Tags

myc tag (9x)

Available since

Dec. 20, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pUbC-OsTIR1-myc-IRES-scFv-sfGFP

Plasmid

#84563

Purpose

Bicistronic vector for expression of O. sativa E3 ubiquitin ligase and single chain variable fragment of GCN4 antibody fused to superfolder GFP

Depositor

Robert Singer

Publication

Wu et al Science. 2016 Jun 17;352(6292):1430-5. d

Insert

Transport inhibitor response 1 (E3 ligase domain), IRES, single chain variable fragment-superfolder GFP

Use

Lentiviral

Tags

myc tag (9x)

Available since

Dec. 20, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

Enlarge