We narrowed to 16,598 results for: LEA
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pICSL11060
Plasmid#68264PurposeLevel 1 Golden Gate Cassette: Cas9 expression cassette for plants (exemplified in Brassica oleracea)DepositorInsertPromoter/5UTR: CsVMV+ CDS:SpCas9 + 3UTR/terminator:35s
UseSynthetic BiologyExpressionPlantAvailable SinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHR-HP1α-miRFP670-Cry2WT
Plasmid#122442PurposeExpresses disordered protein HP1α fused with fluorescent protein miRFP670 and optogenetic protein Cry2WTDepositorInsertHP1 alpha (CBX5 Human)
UseLentiviralTagsCry2WT and miRFP670ExpressionMammalianPromoterSFFVAvailable SinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
B-Sniper SpCas9
Plasmid#207361PurposeExpression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variantDepositorInsertB-Sniper SpCas9
UseCRISPRTags3xFLAGExpressionMammalianMutationF539S, M763I, K890N, amino acids 1005-1013 replac…PromoterCBhAvailable SinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBK092
Plasmid#183146PurposepET-6xHis-MBP-TEV-CrtTIR-APAZ/CrtAgo - Heterologous CrtSPARTA expressionDepositorInsertCrtSPARTA operon (6xHis-MBP-CrtTIR-APAZ and CrtAgo)
Tags6xHis and MBPExpressionBacterialPromoterT7Available SinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
GFP-BLM DM
Plasmid#80071PurposeExpression of a sumo-mutant form GFP-BLM with K317R and K331R sumo-site mutationsDepositorInsertBLM (BLM Human)
TagsGFP-BLMExpressionMammalianMutationK317R (AAA>AGA) and K331R (AAA>AGA)Available SinceJuly 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pT2-TetR-neoR
Plasmid#153310PurposeTet-repressor with neomycin selection marker, to be used together with pT2-CMV/TetO2-EYFP-GWDepositorInsertTetR
UseTransposonPromoterSV40Available SinceAug. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
TVBB N-term-mScarlet
Plasmid#169218PurposeTargeting vector backbone to support a knock-in of mScarlet-Linker at the N-terminus of a target locusDepositorInsertDouble SapI flanked mScarlet
UseTargeting vector backboneAvailable SinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSynapsin_NLS_GFP-P2A_SNAP_GluA2
Plasmid#170455PurposeExpresses SNAP tagged GluA2 in neuronal cellsDepositorAvailable SinceJuly 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-sCreDIO hChR2(H134R)-EYFP
Plasmid#55642PurposesCre-Dependent ChR2-EYFPDepositorInserthChR2(H134R)-EYFP
UseAAV; Scre-dependentTagsEYFPExpressionMammalianPromoterEf1aAvailable SinceAug. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLKO_Ollas.S.V5_mCherry-NLS
Plasmid#178211PurposeNuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.DepositorInsertnPC Tagged mCherry-NLS
UseLentiviralTagsNuclear Localization Signal and Ollas.S.V5PromotereF1aAvailable SinceJuly 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAR100
Plasmid#180377PurposepAR100 allows cloning of TCRα and TCRβ genes using restriction enzymes (AflII, ApaI, and SacII).DepositorInsertsNFAT-Luciferase
hCD8A
ExpressionBacterial and MammalianPromoterhEF1-HTLV and minimal promoter and an NFAT respon…Available SinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFPN1-N-gamma-tubulin (334-449)
Plasmid#87859PurposeFluorescent fragment of gamma-tubulin containing gamma-tubulin DNA binding domain and NLSDepositorInsertgamma-tubulin 1 (TUBG1 Human)
TagsGFPExpressionMammalianMutationfragment from aa 334 to 449Available SinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG-MCP-SRSF3-V5-mCherry
Plasmid#235090PurposeSRSF3 mCherry fusion protein with MCP domainDepositorAvailable SinceMay 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV_eA3A T31A-NG-nCas9
Plasmid#163539PurposeMammalian CG-to-GC base editingDepositorInserteA3A T31A-NG-nCas9
UseCRISPRExpressionMammalianMutationnCas9 (D10A)eA3A T31ANG (L111R, D1135V, G1218R, E…Available SinceDec. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV_APOBEC1-UdgX-NG-nCas9
Plasmid#163533PurposeMammalian CG-to-GC base editingDepositorInsertAPOBEC1-UdgX-NG-nCas9
UseCRISPRExpressionMammalianMutationnCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…Available SinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV_UdgX-APOBEC1-UdgX-HF-nCas9
Plasmid#163559PurposeMammalian CG-to-GC base editingDepositorInsertUdgX-APOBEC1-UdgX-HF-nCas9
UseCRISPRExpressionMammalianMutationnCas9 (D10A)HF (N497A, R661A, Q695A, Q926A)Available SinceJan. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
ROSA26(MultiFPsΔPuro)
Plasmid#140759PurposeTargeting vector to Gt(ROSA)26 locus for conditional expression of distinct FPs (Venus, mCherry, and mCerulean) responding to each site-specific-recombinase activity (Cre, Dre, and phiC31o).DepositorInsertspuromycin-N-acetyltransferase
Venus
mCherry
mCerulean
UseMouse TargetingPromoterCAGGSAvailable SinceJune 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
3xNLS-NLP-cMyc-cMyc LbaCas12a
Plasmid#182125PurposepET21a protein expression vector for 3xNLS-NLP-cMyc-cMyc LbaCas12a in bacteriaDepositorInsert3xNLS-NLP-cMyc-cMyc LbaCas12a
Tags6xHisExpressionBacterialPromoterT7Available SinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
LentiU6-LacZ-GFP-Puro (BB)
Plasmid#170459PurposeFor cloning gRNA blockDepositorInserthU6-BB-LacZ and EF1A-EGFP-2A-Puro
UseLentiviralExpressionMammalianPromoterhU6 for gRNA and EF1A for EGFP-2A-PuroAvailable SinceAug. 12, 2021AvailabilityAcademic Institutions and Nonprofits only
