166,461 results

1. pLVpuro-CMV-N-3xFLAG

   Plasmid

   #123223

   Purpose

   N-terminal 3xFLAG tag. Gateway destination vector for lentivirus production and stable transduction in mammalian cells.

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Type

   Empty backbone

   Use

   Lentiviral; Gateway destination

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. Hsp68-LacZ-Gateway

   Plasmid

   #37843

   Depositor

   Nadav Ahituv

   Article

   Pennacchio et al Nature. 2006 Nov 23;444(7118):499-502. E

   Type

   Empty backbone

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Mouse Hsp68 minimal promoter

   Available Since

   Sept. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. SV40 1: pBSSVD2005

   Plasmid

   #21826

   Depositor

   David Ron

   Insert

   SV40 Large T (SV40gp6 simian virus)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   likely expresses aa109-708 of the large T antigen

   Promoter

   Available Since

   Sept. 14, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. MAC_N_KLF9

   Plasmid

   #167774

   Purpose

   Expresses KLF9 in mammalian cells

   Depositor

   Markku Varjosalo

   Article

   Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

   Insert

   KLF9 (KLF9 Human)
   

   Use

   Tags

   MAC

   Expression

   Mammalian

   Mutation

   no mutations

   Promoter

   Available Since

   Jan. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEX-6p1-GST-ArcFL

   Plasmid

   #119877

   Purpose

   Bacterial Expression construct for protein production and purification

   Depositor

   Jason Shepherd

   Article

   Pastuzyn et al Cell. 2018 Mar 22;173(1):275. doi: 10.10

   Insert

   Activity Regulated Cytoskeletal Protein (Arc Rat)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available Since

   March 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28-AviTag-DogCatcher-MBP

   Plasmid

   #171928

   Purpose

   Expresses AviTag-DogCatcher-MBP in bacterial cytoplasm. The AviTag enables site-specific biotinylation by BirA.

   Depositor

   Mark Howarth

   Article

   Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

   Insert

   AviTag-DogCatcher-MBP
   

   Use

   Tags

   His6 and Thrombin cleavage site

   Expression

   Bacterial

   Mutation

   DogCatcher added to N-terminus of MBP via a GSGES…

   Promoter

   T7

   Available Since

   July 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-Ef1a-mCherry-IRES-Cre

   Plasmid

   #55632

   Purpose

   Expresses Cre in Mammalian Cells

   Depositor

   Karl Deisseroth

   Article

   Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

   Has Service

   AAV Retrograde and AAV8

   Inserts

   mCherry
   Cre
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a and Ef1a/IRES

   Available Since

   July 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFC332

   Plasmid

   #87845

   Purpose

   AMA1 plasmid with Aspergillus optimized Cas9 and hph selection marker

   Depositor

   Uffe Mortensen

   Article

   Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do

   Inserts

   Cas9
   hph (hygromycin resistance marker)
   

   Use

   CRISPR; Fungal expression

   Tags

   SV40 NLS

   Expression

   Bacterial

   Mutation

   Promoter

   Aspergillus nidulans tef1 promoter and Aspergillu…

   Available Since

   May 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHR_SFFV

   Plasmid

   #79121

   Purpose

   Lentiviral vector for constitutive expression of genes (SFFV promoter)

   Depositor

   Wendell Lim

   Article

   Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGWB551

    Plasmid

    #74881

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with G3GFP (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available Since

    July 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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