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pcDNA3.1-HA-GTSE1

Plasmid

#224719

Purpose

For expression of HA-tagged GTSE1 protein

Depositor

Michele Pagano

Article

Garcia-Vazquez et al bioRxiv [Preprint]. 2025 Jan 23:2024.06.

Insert

GTSE1 (GTSE1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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pLKO.1 puro_shDDX58_230212

Plasmid

#167293

Purpose

Lentiviral plKO.1 construct coding for a short-hairpin RNA targeting the human gene DDX58 (coding for RLR sensor RIG-I). Contains a puro resistance cassette.

Depositor

Agnel Sfeir

Article

Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

Insert

shRNA hsDDX58 (RIGI Human)

Use

Lentiviral

Expression

Mammalian

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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pSpCas9nucl-T2A-mCherry - BAKgRNA1- BAKgRNA2

Plasmid

#167295

Purpose

Plasmid encoding for 2 gRNAs targeting the human BAK gene and a CMV driven nuclease Cas9 followed by self-cleaving mCherry

Depositor

Agnel Sfeir

Article

Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

Insert

BAK (BAK1 Human)

Expression

Mammalian

Available Since

April 22, 2021

Availability

Academic Institutions and Nonprofits only

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pCT-CMV-UBE3B(C1036A)-copGFP-Puro

Plasmid

#107344

Purpose

Expresses UBE3B mutant C1036A fused to copGFP in mammalian cells.

Depositor

Robert Sobol

Article

Braganza et al J Biol Chem. 2016 Dec 21. pii: jbc.M116.

Insert

UBE3B(C1036A)copGFP (UBE3B Human)

Use

Lentiviral

Tags

copGFP

Expression

Mammalian

Mutation

mutation C1036A

Promoter

CMV

Available Since

April 19, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pRetroX-TRE3G-IRSp53 S269D

Plasmid

#221319

Purpose

Dox-inducible expression of IRSp53 mutant in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

IRSp53 (Baiap2 Mouse)

Use

Retroviral

Tags

3xFLAG

Mutation

S269D

Promoter

TRE3G

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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p7140 pHAGE-P-CMVt-N-HA-GAW-EME1

Plasmid

#100150

Purpose

Expresses EME1

Depositor

Peter Howley

Article

Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

Insert

EME1 (EME1 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pRetroX-TRE3G_NT-GSDMD_I105N_

Plasmid

#201174

Purpose

Dox-inducible expression of NT-GSDMD gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Cell Rep. 2023 Jan 31;42(1):112008. doi:

Insert

Gasdermin D N-terminal domain (Gsdmd Mouse)

Use

Retroviral

Mutation

aa1-276 only, I105N

Promoter

TRE3G

Available Since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9nucl-T2A-EGFP - BAXgRNA1- BAXgRNA2

Plasmid

#167296

Purpose

Plasmid encoding for 2 gRNAs targeting the human BAX gene and a CMV driven nuclease Cas9 followed by self-cleaving EGFP

Depositor

Agnel Sfeir

Article

Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

Insert

BAX (BAX Human)

Expression

Mammalian

Available Since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

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p7058 pHAGE-P-CMVt-N-HA-GAW-FDFT1

Plasmid

#100151

Purpose

Expresses FDFT1

Depositor

Peter Howley

Article

Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

Insert

FDFT1 (FDFT1 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pOTTC1706 - pAAV mGrid1 390F gRNA EF1a EGFP-KASH

Plasmid

#131683

Purpose

An adeno-associated viral vector expressing nuclear envelope-embedded eGFP and a guide RNA for mGrid1

Depositor

Christopher Richie

Article

Gantz et al Elife. 2020 Apr 1;9. pii: 56054. doi: 10

Inserts

EGFP-KASH
SpCas9 sgRNA vs mouse GRID1

Use

AAV

Tags

KASH

Promoter

EF1a and mU6

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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