We narrowed to 23,366 results for: Nov;

1. pQmod2E-GG

   Plasmid

   #191345

   Purpose

   Clostridium expression vector (pBP1 origin, ermR) with drop-out Plac-RFP cassette flanked with BsaI sites

   Depositor

   Andrew Tolonen

   Article

   Rostain et al ACS Synth Biol. 2022 Dec 16;11(12):4077-

   Insert

   Plac-RFP (IPTG-inducible red fluorescent protein)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Plac

   Available since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGM32DEST

   Plasmid

   #74747

   Purpose

   Encodes for the QF-GR recombinant gene, splice linker, and mCherry reporter gene

   Depositor

   Jordan Ward, Keith Yamamoto

   Article

   Monsalve et al Genetics. 2019 Feb;211(2):419-430. doi:

   Inserts

   Gateway cassette
   QF-GR (NR3C1 Synthetic, Human, Neurospora crassa)
   

   Use

   Tags

   Fusion of the Neurospora crassa QF activator to t…

   Expression

   Worm

   Mutation

   Promoter

   No promoter

   Available since

   Oct. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFX-mCherry-Rab11

   Plasmid

   #174461

   Purpose

   Express mCherry with recycling endosome-targeting sequence in mammalian cells

   Depositor

   Yusuke Ohba

   Article

   Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

   Insert

   mCherry with recycling endosome-targeting sequence
   

   Use

   Tags

   small GTPase (Rab11)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFX-mCherry-Rab7

   Plasmid

   #174458

   Purpose

   Express mCherry with late endosome-targeting sequence in mammalian cells

   Depositor

   Yusuke Ohba

   Article

   Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

   Insert

   mCherry with late endosome-targeting sequence
   

   Use

   Tags

   small GTPase (Rab7)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFX-EGFP-Rab11

   Plasmid

   #174460

   Purpose

   Express iRFP with recycling endosome-targeting sequence in mammalian cells

   Depositor

   Yusuke Ohba

   Article

   Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

   Insert

   EGFP with recycling endosome-targeting sequence
   

   Use

   Tags

   small GTPase (Rab11)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDR-GFPuniv

   Plasmid

   #46085

   Purpose

   In vivo recombination assay fluorescent reporter

   Depositor

   Raymond Monnat

   Article

   Li et al Nucleic Acids Res. 2012 Mar;40(6):2587-9

   Insert

   DR-GFPuniv reporter
   

   Use

   Recombination reporter plasmid

   Tags

   Expression

   Mammalian

   Mutation

   E5G and K163R mutations in EGFP relative to wild-…

   Promoter

   Available since

   May 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCR8/GW_TOPO_Cdk12_Untagged

   Plasmid

   #127176

   Purpose

   Untagged mouse Cdk12 transgene (NM_001109626.1 isoform) cloned into Gateway entry vector

   Depositor

   Phil Sharp

   Article

   Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:

   Insert

   Mouse Cyclin Dependent Kinase 12 (Cdk12 Mouse)
   

   Use

   Entry vector with transgene for gateway cloning

   Tags

   None

   Expression

   Mutation

   Promoter

   None

   Available since

   July 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. ALC1-c005

   Plasmid

   #173092

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   CHD1L (CHD1L Human)
   

   Use

   Tags

   mCerulean

   Expression

   Bacterial

   Mutation

   L613-K879, codon optimized

   Promoter

   Available since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pQmod3E-GG

   Plasmid

   #191348

   Purpose

   Clostridium expression vector (pCB102 origin, ermR) with drop-out Plac-RFP cassette flanked with BsaI sites

   Depositor

   Andrew Tolonen

   Article

   Rostain et al ACS Synth Biol. 2022 Dec 16;11(12):4077-

   Insert

   Plac-RFP (IPTG-inducible red fluorescent protein)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Plac

   Available since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pQmod4E-GG

    Plasmid

    #191351

    Purpose

    Clostridium expression vector (pCD6 origin, ermR) with drop-out Plac-RFP cassette flanked with BsaI sites

    Depositor

    Andrew Tolonen

    Article

    Rostain et al ACS Synth Biol. 2022 Dec 16;11(12):4077-

    Insert

    Plac-RFP (IPTG-inducible red fluorescent protein)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Plac

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. ARH1-c003

    Plasmid

    #173093

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    ADPRH (ADPRH Human)
    

    Use

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    E2-L357, codon optimized

    Promoter

    Available since

    Nov. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFX-H2B-iRFP

    Plasmid

    #175842

    Purpose

    Express iRFP with nucleus-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    iRFP with nucleus-targeting sequence
    

    Use

    Tags

    H2B (human histone 2B)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTS95 pHAGE2 CMVtetO2 GFP-22xGS-TEV-MCS

    Plasmid

    #199348

    Purpose

    Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-TEV-tagged protein in mammalian cells.

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    EGFP-TEV

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pFX-mito-roGFP

    Plasmid

    #174181

    Purpose

    Express roGFP with mitochondria-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    roGFP with mitchondria-targeting sequence
    

    Use

    Tags

    the mitochondria-targeting sequence of the cytoch…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFX-H2B-Venus

    Plasmid

    #175840

    Purpose

    Express Venus with nucleus-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    Venus with nucleus-targeting sequence
    

    Use

    Tags

    H2B (human histone 2B)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. MacroH2A.2-c002

    Plasmid

    #173089

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    MACROH2A2 (MACROH2A2 Human)
    

    Use

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    K167-K372

    Promoter

    Available since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFX-GalT-EGFP

    Plasmid

    #174471

    Purpose

    Express EGFP with golgi apparatus-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    EGFP with golgi apparatus-targeting sequence
    

    Use

    Tags

    β1,4-galactose transferase

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTS107 pHAGE2 CMVtetO2 MCS-TEV-SUMOEu-22xGS-GFP

    Plasmid

    #199360

    Purpose

    Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally TEV-SUMOEu-GFP-tagged protein in mammalian cells.

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    TEV-SUMOEu-EGFP

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pPBJ pFOS-KTR-Gal4-PEST-tub3’ pCMV-TagBFP

    Plasmid

    #176531

    Purpose

    PiggyBAC vector expressing a FOS promoter driven "KGV" synthetic transcription factor and a CMV promoter driven BFP marker.

    Depositor

    Jared Toettcher

    Article

    Ravindran et al Cell Syst. 2022 Feb 16;13(2):131-142.e13

    Inserts

    KGV transcription factor
    TagBFP
    

    Use

    Piggybac transposase integration vector

    Tags

    ErkKTR, Gal4 DNA binding domain, PEST destabiliza…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pYZ_3WJrep_N20_switch

    Plasmid

    #132722

    Purpose

    T7 RNAP-driven expression of 3WJ repressor index 20 switch RNA with a GFP-ASV reporter

    Depositor

    Peng Yin, Alexander Green

    Article

    Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

    Insert

    3WJ_N20_switch
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pYZ_3WJrep_N20_trigger

    Plasmid

    #132726

    Purpose

    T7 RNAP-driven expression of 3WJ repressor index 20 trigger RNA

    Depositor

    Peng Yin, Alexander Green

    Article

    Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

    Insert

    3WJ_N20_trigger
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pYZ_3WJrep_N24_switch

    Plasmid

    #132725

    Purpose

    T7 RNAP-driven expression of 3WJ repressor index 24 switch RNA with a GFP-ASV reporter

    Depositor

    Peng Yin, Alexander Green

    Article

    Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

    Insert

    3WJ_N24_switch
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3.1_GEM4-Halo-FRB

    Plasmid

    #197057

    Purpose

    Expression of Paraburkholderia xenovorans encapsulin Halo-FRB-fusion in mammalian cells

    Depositor

    Julia Mahamid

    Article

    Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

    Insert

    Encapsulin
    

    Use

    Tags

    FRB and HaloTag

    Expression

    Mammalian

    Mutation

    Codon-optimised for human cell expression

    Promoter

    Available since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3.1_GEM7-Halo-FRB

    Plasmid

    #197058

    Purpose

    Expression of Rhodospirillum rubrum encapsulin Halo-FRB-fusion in mammalian cells

    Depositor

    Julia Mahamid

    Article

    Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

    Insert

    Encapsulin
    

    Use

    Tags

    FRB and HaloTag

    Expression

    Mammalian

    Mutation

    Codon-optimised for human cell expression

    Promoter

    Available since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA3.1_GEM22-Halo-FRB

    Plasmid

    #197059

    Purpose

    Expression of Mycobacterium hassiacum encapsulin Halo-FRB-fusion in mammalian cells

    Depositor

    Julia Mahamid

    Article

    Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

    Insert

    Encapsulin
    

    Use

    Tags

    FRB and HaloTag

    Expression

    Mammalian

    Mutation

    Codon-optimised for human cell expression

    Promoter

    Available since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pBMN PLEKHM1-HA

    Plasmid

    #89300

    Purpose

    For expression of PLEKHM1 tagged with HA

    Depositor

    Michael Lazarou

    Article

    Nguyen et al J Cell Biol. 2016 Dec 19;215(6):857-874.

    Insert

    PLEKHM1 (PLEKHM1 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. PB-Gata2

    Plasmid

    #60665

    Purpose

    PiggyBac expression vector encoding rat Gata2 downstream of a doxycycline inducible promoter

    Depositor

    James Thomson

    Article

    Vereide et al Stem Cell Reports. 2014 Dec 9;3(6):1043-

    Insert

    Gata2 (Gata2 Rat)
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. ARH3-c002

    Plasmid

    #173095

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    ADPRS (ADPRS Human)
    

    Use

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    A14-S363

    Promoter

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA5/FRT/TO/FLAG-HA-SBP-TNRC6A-NES-mut

    Plasmid

    #42046

    Depositor

    Kumiko Ui-Tei

    Article

    Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

    Insert

    TNRC6A (TNRC6A Human)
    

    Use

    Tags

    FLAG, HA, and SBP

    Expression

    Mammalian

    Mutation

    F951A, F955A, I958A, F960A

    Promoter

    Available since

    March 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAAVS1_EF1a-MitoTrap-mCherry-IRES-Puro

    Plasmid

    #197067

    Purpose

    Donor plasmid for human AAVS1 locus knock-in, constitutive expression of MitoTrap-mCherry

    Depositor

    Julia Mahamid

    Article

    Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

    Insert

    MitoTrap-mCherry
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge