We narrowed to 257,083 results for: CIL

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pLenti-PE2-BSD

Plasmid

#161514

Purpose

Expression of PE2 in mammalian cells. Can be used to generate stable cell lines by Blasticidin selection

Depositor

Hyongbum Kim

Publication

Kim et al Nat Biotechnol. 2020 Sep 21. pii: 10.103

Insert

PE2-P2A-BSD

Use

Lentiviral

Tags

SV40 NLS

Expression

Mammalian

Promoter

EF-1α core promoter

Available since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pEN396 - pCAGGS-Tir1-V5-2A-PuroR TIGRE donor

Plasmid

#92142

Purpose

Targeting vector to introduce an osTir1 expressing cassette at the mouse TIGRE acceptor locus using Puromycin selection (2A-fusion). Auxin-inducible degron system.

Depositor

Benoit Bruneau

Publication

Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

osTir1

Use

Mouse Targeting

Tags

V5 tag

Expression

Mammalian

Available since

June 26, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pEN396 - pCAGGS-Tir1-V5-2A-PuroR TIGRE donor

Plasmid

#92142

Purpose

Targeting vector to introduce an osTir1 expressing cassette at the mouse TIGRE acceptor locus using Puromycin selection (2A-fusion). Auxin-inducible degron system.

Depositor

Benoit Bruneau

Publication

Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

osTir1

Use

Mouse Targeting

Tags

V5 tag

Expression

Mammalian

Available since

June 26, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pCAG-mTagBFP2

Plasmid

#122373

Purpose

Expresses mTagBFP2 in mammalian cells

Depositor

Hiroshi Ochiai

Publication

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mTagBFP2

Expression

Mammalian

Available since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCAG-mTagBFP2

Plasmid

#122373

Purpose

Expresses mTagBFP2 in mammalian cells

Depositor

Hiroshi Ochiai

Publication

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mTagBFP2

Expression

Mammalian

Available since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pGEx-4T-1-YTHDF1

Plasmid

#70087

Purpose

Encode YTHDF1 with N-terminal GST tag for E. coli expression

Depositor

Chuan He

Publication

Wang et al Nature. 2014 Jan 2;505(7481):117-20. doi

Insert

YTH N(6)-methyladenosine RNA binding protein 1 (YTHDF1 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pGEx-4T-1-YTHDF1

Plasmid

#70087

Purpose

Encode YTHDF1 with N-terminal GST tag for E. coli expression

Depositor

Chuan He

Publication

Wang et al Nature. 2014 Jan 2;505(7481):117-20. doi

Insert

YTH N(6)-methyladenosine RNA binding protein 1 (YTHDF1 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pBS130

Plasmid

#26290

Depositor

Tom Clandinin

Publication

Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

Insert

phiC31 integrase (int Streptomyces phage phiC31)

Use

Drosophila transgenesis

Expression

Bacterial and Insect

Available since

Feb. 2, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

19 Articles

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pBS130

Plasmid

#26290

Depositor

Tom Clandinin

Publication

Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

Insert

phiC31 integrase (int Streptomyces phage phiC31)

Use

Drosophila transgenesis

Expression

Bacterial and Insect

Available since

Feb. 2, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

19 Articles

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AAV-GfaABC1D-MCS--4x6T-WPRE

Plasmid

#196417

Purpose

Empty vector for astrocyte-selective expression in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassette

Depositor

Stanley Thomas Carmichael

Publication

Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

Type

Empty backbone

Use

AAV; Astrocyte-selective

Expression

Mammalian

Promoter

GfaABC1D

Available since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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AAV-GfaABC1D-MCS--4x6T-WPRE

Plasmid

#196417

Purpose

Empty vector for astrocyte-selective expression in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassette

Depositor

Stanley Thomas Carmichael

Publication

Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

Type

Empty backbone

Use

AAV; Astrocyte-selective

Expression

Mammalian

Promoter

GfaABC1D

Available since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDG458

Plasmid

#100900

Purpose

SpCas9 with 2A-EGFP and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.

Depositor

Paul Thomas

Publication

Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do

Inserts

humanized CRISPR associated protein 9
U6-gRNA scaffold 1
U6-gRNA scaffold 2

Use

CRISPR and Mouse Targeting

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh and U6

Available since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

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pDG458

Plasmid

#100900

Purpose

SpCas9 with 2A-EGFP and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.

Depositor

Paul Thomas

Publication

Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do

Inserts

humanized CRISPR associated protein 9
U6-gRNA scaffold 1
U6-gRNA scaffold 2

Use

CRISPR and Mouse Targeting

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh and U6

Available since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

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pCS2+HyPer7-NLS

Plasmid

#136468

Purpose

Mammalian expression of nucleus targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

Depositor

Vsevolod Belousov

Publication

Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

HyPer7

Tags

Triple nuclear localization signal of SV40 (simia…

Expression

Mammalian

Promoter

CMV, SP6

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pCS2+HyPer7-NLS

Plasmid

#136468

Purpose

Mammalian expression of nucleus targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

Depositor

Vsevolod Belousov

Publication

Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

HyPer7

Tags

Triple nuclear localization signal of SV40 (simia…

Expression

Mammalian

Promoter

CMV, SP6

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pLenti-Rac1-2G

Plasmid

#66111

Purpose

Second generation Rac1 FRET biosensor for lentivirus production

Depositor

Olivier Pertz

Publication

Fritz et al Dev Cell. 2015 Sep 29. pii: S1534-5807(1

Insert

Second generation Rac1 FRET biosensor

Use

Lentiviral

Tags

His-Myc

Expression

Mammalian

Promoter

CMV

Available since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pLenti-Rac1-2G

Plasmid

#66111

Purpose

Second generation Rac1 FRET biosensor for lentivirus production

Depositor

Olivier Pertz

Publication

Fritz et al Dev Cell. 2015 Sep 29. pii: S1534-5807(1

Insert

Second generation Rac1 FRET biosensor

Use

Lentiviral

Tags

His-Myc

Expression

Mammalian

Promoter

CMV

Available since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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GFP-pGBD

Plasmid

#26735

Depositor

William Bement

Publication

Benink et al J Cell Biol. 2005 Jan 31. 168(3):429-39.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Rac (rac1 Frog)

Use

Reporter for rac

Tags

GFP

Expression

Mammalian

Mutation

Rac binding domain of rhotekin

Available since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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GFP-pGBD

Plasmid

#26735

Depositor

William Bement

Publication

Benink et al J Cell Biol. 2005 Jan 31. 168(3):429-39.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Rac (rac1 Frog)

Use

Reporter for rac

Tags

GFP

Expression

Mammalian

Mutation

Rac binding domain of rhotekin

Available since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pLS-mP

Plasmid

#81225

Purpose

Lentivirus enhancer assay plasmid that contains a minimal promoter (mP) and a EGFP reporter gene.

Depositor

Nadav Ahituv

Publication

Inoue et al Genome Res. 2016 Nov 9. pii: gr.212092.1

Type

Empty backbone

Use

Lentiviral

Promoter

Derived from pGL4.23

Available since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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