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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 11,731 results for: EMB

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Showing: 7281 - 7300 of 11731 results

  2. mEos3.2trLATm2allL+A

    Plasmid
    #203750
    Purpose
    Encodes the transmembrane domain from human LAT with mEos3.2 fused on the C terminus. Residues within the transmembrane domain mutated to L and A to increase the TMD interfacial surface area with the membrane. Used as a fluorescent plasma membrane probe.
    Depositor
    Sarah Veatch
    Article
    Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d
    Insert
    trLATm2(allL+A) (LAT Human)
    Tags
    mEos3.2
    Expression
    Mammalian
    Available Since
    Aug. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    203750-plasmid-map-sequence-id-403335

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  6. mEos3.2trLAT-P

    Plasmid
    #203747
    Purpose
    Encodes the transmembrane domain from mouse LAT with mEos3.2 fused on the N terminus. The palmitoylation site at C27 is mutated to A. Used as a fluorescent plasma membrane probe.
    Depositor
    Sarah Veatch
    Article
    Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d
    Insert
    trLAToC27A (Lat Mouse)
    Tags
    ER translocation signal - mEos3.2
    Expression
    Mammalian
    Mutation
    C27A
    Available Since
    Aug. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    203747-plasmid-map-sequence-id-403346

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  11. PCUP1-GFP-Pho8-406

    Plasmid
    #207006
    Purpose
    Integration of GFP-Pho8 as an additional copy in genome, expressed under CUP1 promoter. Uses auxotrophic marker URA3 (Saccharomyces cerevisiae) for selection. Marker for yeast vacuolar membrane.
    Depositor
    Zhiping Xie
    Article
    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.
    Insert
    PHO8
    Tags
    GFP
    Expression
    Yeast
    Available Since
    June 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    207006-plasmid-map-sequence-id-422251

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  12. PCUP1-GFP-Osh1-406

    Plasmid
    #207007
    Purpose
    Integration of Osh1-GFP as an additional copy in genome, expressed under CUP1 promoter. Uses auxotrophic marker URA3 (Saccharomyces cerevisiae) for selection. Yeast PMN pathway substrate.
    Depositor
    Zhiping Xie
    Article
    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.
    Insert
    OSH1
    Tags
    GFP
    Expression
    Yeast
    Available Since
    April 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    207007-plasmid-map-sequence-id-422254

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  14. trLATm1PtoLmEos3.2

    Plasmid
    #203749
    Purpose
    Encodes the transmembrane domain from human LAT with mEos3.2 fused on the C terminus. Prolines P8 and P17 in the LAT transmembrane domain mutated to L. Used as a fluorescent plasma membrane probe.
    Depositor
    Sarah Veatch
    Article
    Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d
    Insert
    trLATm1 (PtoL) (LAT Human)
    Tags
    mEos3.2
    Expression
    Mammalian
    Mutation
    P8L, P17L
    Available Since
    Aug. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    203749-plasmid-map-sequence-id-403341

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  18. TP1396

    Plasmid
    #254447
    Purpose
    Lentiviral expression construct for a 3xFLAG-BFP and ALFA-tagged anti-MBP nanobody driven by EF1α promoter.
    Depositor
    Tino Pleiner
    Article
    Stanton et al bioRxiv [Preprint]. 2026 Jan 15:2026.01.
    Insert
    anti-MBP nanobody
    Use
    Lentiviral
    Mutation
    Contains engineered nanobody variant with ALFA ta…
    Promoter
    EF1α
    Available Since
    May 15, 2026
    Availability
    Academic Institutions and Nonprofits only
    254447-plasmid-map-sequence-id-509533

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  20. pHS1625

    Plasmid
    #239834
    Purpose
    OrufIscB-REC E. coli expression for recombinant purification
    Depositor
    Feng Zhang
    Article
    Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038
    Insert
    OrufIscB-REC
    Tags
    14xHis-TwinStrep-bdSUMO
    Expression
    Bacterial
    Mutation
    Insertion of REC domain from NbaCas9-1
    Promoter
    T7
    Available Since
    July 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    239834-plasmid-map-sequence-id-479361

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Showing: 7281 - 7300 of 11731 results