We narrowed to 15,989 results for: LEA

1. pENTR-KAT14-547-782

   Plasmid

   #118376

   Purpose

   Gateway compatible donor vector with KAT14-547-782 truncation.

   Depositor

   Maria Vartiainen

   Article

   Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

   Insert

   KAT14 (KAT14 Human)
   

   Use

   Donor-plasmid

   Mutation

   Truncation (aa 547-782)

   Available Since

   June 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pENTR-KAT14-1-546

   Plasmid

   #118377

   Purpose

   Gateway compatible donor vector with KAT14-1-546 truncation.

   Depositor

   Maria Vartiainen

   Article

   Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

   Insert

   KAT14 (KAT14 Human)
   

   Use

   Donor-plasmid

   Mutation

   Truncation (aa 1-546)

   Available Since

   Nov. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTKan-p35S::Csy4-pC4H::cogRFP (C90, JBEI-15908)

   Plasmid

   #110143

   Purpose

   Transformation and expression of Csy4 and cogDsRed proteins in plants

   Depositor

   Dominique Loque

   Article

   Liang et al ACS Synth Biol. 2017 May 19;6(5):806-816

   Insert

   Csy4 (ATCS Mustard Weed)
   

   Use

   Binary vector

   Tags

   cogDsRed

   Expression

   Bacterial and Plant

   Available Since

   June 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTKan-p35S::Csy4-pC4H::cogGFP (C44, JBEI-16338)

   Plasmid

   #110144

   Purpose

   Transformation and expression of Csy4 and cogGFP proteins in plants

   Depositor

   Dominique Loque

   Article

   Liang et al ACS Synth Biol. 2017 May 19;6(5):806-816

   Insert

   Csy4 (ATCS Mustard Weed)
   

   Tags

   cogGFP

   Expression

   Bacterial and Plant

   Available Since

   June 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pQCXIX_sh_hMIB2_#9

   Plasmid

   #75255

   Purpose

   retroviral shRNA vector against human MIB2, expresses GFP for transduction control

   Depositor

   Ria Baumgrass

   Article

   Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

   Insert

   shRNA against MIB2 (human) (MIB2 Human)
   

   Use

   RNAi and Retroviral

   Expression

   Mammalian

   Promoter

   mU6

   Available Since

   June 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. mGDF8 donor CFP-hDF

   Plasmid

   #72590

   Purpose

   Plasmid contains left and right homology arms for mouse GDF8 flanking the mCherry-puromycin-2A-ECFP-dysferlin cassette.

   Depositor

   Renzhi Han

   Article

   Xu et al Mol Ther Nucleic Acids. 2013 Jul 30;2:e1

   Insert

   dysferlin (DYSF Human)
   

   Tags

   ECFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. hGDF8 donor CFP-hDF

   Plasmid

   #72591

   Purpose

   Plasmid contains left and right homology arms for human GDF8 flanking the mCherry-puromycin-2A-ECFP-dysferlin cassette.

   Depositor

   Renzhi Han

   Article

   Xu et al Mol Ther Nucleic Acids. 2013 Jul 30;2:e1

   Insert

   dysferlin (DYSF Human)
   

   Tags

   ECFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. Hoxb6-3'UTR

   Plasmid

   #31523

   Depositor

   Chang-Zheng Chen

   Article

   Arnold et al Genome Res. 2011 May . 21(5):798-810.

   Insert

   Hoxb6-3'UTR (Hoxb6 Mouse)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Available Since

   Oct. 4, 2011

   Availability

   Academic Institutions and Nonprofits only

9. EGFP-BRCA1

   Plasmid

   #115491

   Purpose

   EGFP-BRCA1 expression vector. Parton clone BXY

   Depositor

   Rob Parton

   Article

   McMahon et al Elife. 2021 Jun 18;10. pii: 61407. doi:

   Insert

   BRCA1 (BRCA1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   S1009R (See depositor comments)

   Available Since

   Nov. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-TaqStoffel (JO595)

    Plasmid

    #208952

    Purpose

    A variant CE1 construct with Taq Stoffel DNA polymerase, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    PCV2-XTEN-nSpCas9-BPNLS-TaqStoffel-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A)

    Promoter

    CMV and T7

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-GFP-LC3-RFP-LC3ΔG

    Plasmid

    #168997

    Purpose

    Expresses GFP-LC3-RFP-LC3ΔG in mammalian cells and zebrafish to measure autophagic flux

    Depositor

    Noboru Mizushima

    Article

    Kaizuka et al Mol Cell. 2016 Nov 17;64(4):835-849. doi

    Insert

    microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
    

    Tags

    EGFP and mRFP1

    Expression

    Mammalian

    Available Since

    April 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMRX-IP-GFP-LC3-RFP

    Plasmid

    #84573

    Purpose

    Expresses GFP-LC3-RFP in mammalian cells to measure autophagic flux

    Depositor

    Noboru Mizushima

    Article

    Kaizuka et al Mol Cell. 2016 Nov 17;64(4):835-849. doi

    Insert

    microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
    

    Use

    Retroviral

    Tags

    EGFP and mRFP1

    Expression

    Mammalian

    Available Since

    Jan. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)

    Plasmid

    #182287

    Purpose

    Encodes codon-optimized AF mutant of M. mazei-derived pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF) & tRNACUAPyl used for amber codon suppression in mammalian cells

    Depositor

    Ivana Nikić-Spiegel

    Article

    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

    Inserts

    codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
    PylT
    

    Tags

    nuclear export signal (NES)

    Expression

    Mammalian

    Mutation

    Y306A/Y384F (AF) double mutant of Methanosarcina …

    Promoter

    CMV and U6

    Available Since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. DOX_SAM_CRISPR-activation-2A-GFP-AAVS1-targeting-construct

    Plasmid

    #176836

    Purpose

    Targeting construct for intergrating a doxycycline (DOX) inducible SAM CRISPR activation complex -2A-GFP to the AAVS1 locus.

    Depositor

    Rudolf Jaenisch

    Article

    Ma et al Cell Stem Cell. 2022 Apr 13. pii: S1934-

    Insert

    dCas9-VP64-2A-MS2-p65-HSF1-2A-GFP
    

    Use

    Unspecified

    Mutation

    See Depositor Comments Below

    Promoter

    Tet operator (tetracycline inducible expression)

    Available Since

    Nov. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. Click editor (CE1) - pCMV-T7-PCV2-nCas9-EcKlenow (EMK488)

    Plasmid

    #208942

    Purpose

    The CE1 construct, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    PCV2-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available Since

    Nov. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. OCT4-2A-eGFP-PGK-Puro

    Plasmid

    #31938

    Purpose

    Human OCT4 genomic targeting donor

    Depositor

    Rudolf Jaenisch

    Article

    Hockemeyer et al Nat Biotechnol. 2011 Jul 7. doi: 10.1038

    Insert

    OCT4 (POU5F1 Human)
    

    Use

    Targeting donor

    Tags

    eGFP

    Available Since

    Sept. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-PDGFR-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq-tTA

    Plasmid

    #210501

    Purpose

    expresses LAUNCHER component in mammalian cells

    Depositor

    Dongmin Lee

    Article

    Cui et al Nat Chem Biol. 2024 Mar;20(3):353-364. d

    Insert

    PDGFR-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq-tTA
    

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLVX-ATF4 mScarlet NLS

    Plasmid

    #115969

    Purpose

    ER-stress sensor - ATF4 translation at ORF3 fluorescent reporter with mScarlet-I

    Depositor

    David Andrews

    Article

    Nougarede et al Assay Drug Dev Technol. 2018 Aug 8. doi:

    Insert

    activating transcription factor 4 (ATF4 Human, Synthetic)
    

    Use

    Lentiviral

    Tags

    HA tag, c-myc NLS, and mScarlet-I

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. elavl3:hGCGR-TEVcs-QF;he1.1:CFP

    Plasmid

    #218852

    Purpose

    D. rerio elavl3 promoter driving expression of human glucagon receptor, TEV protease recognition site, QF fusion protein for use in trans-Tango

    Depositor

    Marnie Halpern

    Article

    Coomer et al Nat Neurosci. 2025 Jan;28(1):189-200. do

    Insert

    hGCGR (GCGR Human)
    

    Tags

    QF fusion protein

    Expression

    Bacterial

    Available Since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. RUSH reporter-HMGB1-SBP-GFP

    Plasmid

    #172357

    Purpose

    Monitoring of HMGB1 subcellular localization by fluorescence videomicroscopy

    Depositor

    Guido Kroemer

    Article

    Liu et al Sci Rep. 2017 Nov 2;7(1):14915. doi: 10.

    Insert

    high mobility group box 1 (HMGB1 Human)
    

    Use

    Lentiviral

    Tags

    SBP-GFP

    Promoter

    CMV

    Available Since

    June 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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