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We narrowed to 12,282 results for: LEA

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Showing: 7321 - 7340 of 12282 results

  3. pCVL.SSA TLR (Ani)

    Plasmid
    #45577
    Purpose
    Codes for the SSA-TLR with the target site for I-Ani I in a lentiviral backbone
    Depositor
    Andrew Scharenberg
    Publication
    Kuhar et al Nucleic Acids Res. 2013 Oct 10.
    Inserts
    5' iRFP arm
    eGFP with I-Ani I TS
    +3 mCherry
    3' iRFP arm
    Use
    Lentiviral
    Expression
    Bacterial and Mammalian
    Mutation
    embedded I-Ani I TS from 163-185bp, truncated 25 …
    Promoter
    SFFV
    Available since
    Nov. 4, 2013
    Availability
    Academic Institutions and Nonprofits only
    45577-plasmid-map-sequence-id-67848

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  4. L3MBTL3 (3UT1)

    Plasmid
    #36901
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    L3MBTL3 (L3MBTL3 Human)
    Tags
    His tag and Streptavidin-Binding Peptide (SBP)-Ta…
    Expression
    Bacterial
    Mutation
    contains amino acid residues 228-551 only
    Available since
    June 21, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  8. pET28a(+)_His6_Halo_TEV_SrcSH2pYsuperbinder

    Plasmid
    #260541
    Purpose
    Expresses Halo-SrcSH2 pY superbinder protein in Escherichia coli cells for conjugation to HaloLink beads and phosphotyrosine peptide enrichment.
    Depositor
    Judit Villén
    Publication
    Chang et al EMBO J. 2026 Jul 3. doi: 10.1038/s44318-
    Insert
    Src SH2 pY superbinder
    Use
    Affinity Reagent/ Antibody
    Tags
    HaloTag7, His6, and Tobacco Etch Virus Cleavage s…
    Expression
    Bacterial
    Mutation
    Thr43Val, Cys48Ala, Lys66Leu as identified by ori…
    Promoter
    lacIQ
    Available since
    Aug. 18, 2026
    Availability
    Academic Institutions and Nonprofits only
    260541-plasmid-map-sequence-id-519036

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  9. pRBC-PINBodyN-Tau(1-441)-404TAG

    Plasmid
    #253347
    Purpose
    Express in E.coli, Inclusion body tagged protein expression, Tau 2N4R residues 1-441, TAG codon at residue 404 for phosphoserine encoding
    Depositor
    Richard Cooley
    Publication
    Vesely et al Protein Sci. 2026 Apr;35(4):e70520. doi:
    Insert
    Tau 2N4R S404 TAG (MAPT Human)
    Tags
    PINBodyN (his6-tag included)
    Expression
    Bacterial
    Mutation
    Serine 404 to TAG codon
    Promoter
    T7
    Available since
    May 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    253347-plasmid-map-sequence-id-508709

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  10. pSB-CMV-MCS-Puro mCherry-Sec 61B -P2A-EGFP-LMNB1

    Plasmid
    #250911
    Purpose
    Dual color expression of mCherry tagged human Sec 61B and EGFP tagged zebrafish LaminB1 in mammalian cell lines
    Depositor
    Philipp Niethammer
    Publication
    Shen et al Nat Cell Biol. 2025 Dec 9. doi: 10.1038/
    Insert
    mCherry-Sec61B-P2A-EGFP-LMNB1 (SEC61B, lmnb1 Human, Zebrafish)
    Tags
    EGFP on LMNB1 and mCherry on Sec61b
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    March 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    250911-plasmid-map-sequence-id-501821

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  11. pHBT-KRAS174

    Plasmid
    #248050
    Purpose
    To express His6 tag-Avi tag-TEV cleavage site-KRAS (residues 1-174) in E. coli, under the T7 promoter.
    Depositor
    Shohei Koide
    Publication
    Wallon et al Proc Natl Acad Sci U S A. 2022 Oct 25;11
    Insert
    KRAS (KRAS Human)
    Tags
    His6 tag-Avi tag-TEV cleavage site
    Expression
    Bacterial
    Mutation
    includes aa 1-174 only
    Promoter
    T7
    Available since
    Nov. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    248050-plasmid-map-sequence-id-493232

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  12. pCMV-SpCas9-VQR-P2A-EGFP (LRF199)

    Plasmid
    #242664
    Purpose
    CMV promoter expression plasmid for human codon optimized SpCas9-VQR-BPNLS(SV40)-3xFLAG-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pCMV-SpCas9-VQR-BPNLS(SV40)-3xFLAG-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    VQR mutations in SpCas9(D1135V/R1335Q/T1337R)
    Promoter
    CMV and T7
    Available since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242664-plasmid-map-sequence-id-488971

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  14. pcDNA3.1-myc:Skp1(dC111):gs6:TEVs:gs6:fLuc:HA

    Plasmid
    #228530
    Purpose
    Firefly luciferase in fusion with a fragment of human Skp1 protein through a flexible linker and a TEV viral protease cleavage site. Expression of fLuc protein is deinhibited by TEV site cleavage.
    Depositor
    Roman Jerala
    Publication
    Verbic et al ACS Synth Biol. 2024 Mar 15;13(3):792-80
    Insert
    myc:Skp1(dC111):gs6:TEVs:gs6:fLuc:HA (SKP1 Human, Synthetic, Photinus pyralis)
    Tags
    HA and myc
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Dec. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    228530-plasmid-map-sequence-id-456690

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  15. pEJS2703-CMV-iNme2Smu-ABE8e-nt

    Plasmid
    #228724
    Purpose
    Expresses N-terminal ABE8e-iNme2Smu nCas9 (D16A) base editor in mammalian cells
    Depositor
    Erik Sontheimer
    Publication
    Bamidele et al bioRxiv [Preprint]. 2024 Nov 7:2024.10.3
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    N-terminal ABE8e with iNme2Smu nCas9 (D16A)
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Nme2-Cas9, Smu PID, D16A D844G E868K K870R D873A …
    Promoter
    CMV
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    228724-plasmid-map-sequence-id-454004

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  16. pEJS2714-CMV-iNme2Smu-ABE8e-i1-L10

    Plasmid
    #228735
    Purpose
    Expresses domain inlaid iNme2Smu nCas9 (D16A)-i1-ABE8e base editor with linker-10 in mammalian cells
    Depositor
    Erik Sontheimer
    Publication
    Bamidele et al bioRxiv [Preprint]. 2024 Nov 7:2024.10.3
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Domain inlaid iNme2Smu nCas9 (D16A)-i1-L10
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Nme2-Cas9, Smu PID, D16A D844G E868K K870R D873A …
    Promoter
    CMV
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    228735-plasmid-map-sequence-id-453996

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  17. pEJS2713-CMV-iNme2-ABE8e-i1-L10

    Plasmid
    #228734
    Purpose
    Expresses domain inlaid iNme2 nCas9 (D16A)-i1-ABE8e base editor with linker-10 in mammalian cells
    Depositor
    Erik Sontheimer
    Publication
    Bamidele et al bioRxiv [Preprint]. 2024 Nov 7:2024.10.3
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Domain inlaid iNme2 nCas9 (D16A)-i1-L10
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Nme2-Cas9 D16A D844G E868K K870R D873A D911G K929…
    Promoter
    CMV
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    228734-plasmid-map-sequence-id-453992

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  18. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-M-MLV(WT) (DRR1012)

    Plasmid
    #217799
    Purpose
    Variant CE1 construct with wild-type M-MLV revese transcriptase (RT), expressed from CMV or T7 promoters
    Depositor
    Benjamin Kleinstiver
    Publication
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV2-XTEN-nSpCas9-BPNLS-M-MLV-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A)
    Promoter
    CMV and T7
    Available since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217799-plasmid-map-sequence-id-432866

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Showing: 7321 - 7340 of 12282 results