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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 7,660 results for: Pol

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Showing: 7321 - 7340 of 7660 results

  1. 8V04

    Plasmid
    #220879
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    TMPRSS2 (TMPRSS2 Human)
    Use
    Baculovirus expresssion
    Tags
    EFVEHHHHHHHH and Honeybee melittin signal
    Mutation
    S251D/R252D/Q253D/S254D/R255K
    Promoter
    Polyhedrin
    Available Since
    Sept. 30, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    220879-plasmid-map-sequence-id-436702

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  5. pFBD-BirA_ATG16L1:303-607

    Plasmid
    #210942
    Purpose
    Insect expression of human ATG16L1 fragment G303 to Y607. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.
    Depositor
    Cheryl Arrowsmith
    Article
    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.
    Insert
    ATG16L1:G303-Y607 (ATG16L1 Human)
    Tags
    GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG
    Expression
    Insect
    Mutation
    wild type
    Promoter
    Polyhedrin
    Available Since
    March 4, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    210942-plasmid-map-sequence-id-424570

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  8. pLX304-EZH1-dPSET

    Plasmid
    #203586
    Purpose
    Expresses V5-tagged mutant version of EZH1 with deletion of aa 744-747 and partially resistant to JQ-EZ-05 in mammalian cells.
    Depositor
    Abhishek Chakraborty, William Kaelin
    Article
    Chakraborty et al Sci Transl Med. 2017 Jul 12;9(398):eaal5
    Insert
    EZH1 (EZH1 Human)
    Use
    Lentiviral
    Tags
    V5-tagged
    Expression
    Mammalian
    Mutation
    deletion of aa 744-747 and partial resistance to ā€¦
    Promoter
    CMV
    Available Since
    Feb. 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    203586-plasmid-map-sequence-id-423597

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  16. pCAG-lox-inv[Talpha1-Dre-pA]-lox-mOrange2-Cdk5rap2-CTD

    Plasmid
    #196881
    Purpose
    Neuron-specific expression of the centrosome targeting carboxy-terminal domain (CTD) of Cdk5rap2 fused to mOrange2. Used to displace endogenous Cdk5rap2 from centrosomes.
    Depositor
    Frank Bradke
    Article
    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16
    Insert
    mOrange2-Cdk5rap2-CTD
    Use
    Cre/Lox; Dre/rox
    Expression
    Mammalian
    Promoter
    Quimeric CAG
    Available Since
    May 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    196881-plasmid-map-sequence-id-391885

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  18. pBactin-AcGFP-Cdk5rap2-CTDdeltaCBD

    Plasmid
    #196856
    Purpose
    Expression of the centrosome targeting carboxy-terminal domain (CTD) laking the centrosome binding domain (deltaCBD) of Cdk5rap2 fused to AcGFP. It cannot displace Cdk5rap2 from centrosomes
    Depositor
    Frank Bradke
    Article
    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16
    Insert
    AcGFP-Cdk5rap2-CTDdeltaCBD (Cdk5rap2 Rat)
    Expression
    Mammalian
    Mutation
    Lacking the centrosome-targeting domain (CTD)
    Promoter
    Chicken bactin (plus Chicken bactin intron)
    Available Since
    May 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    196856-plasmid-map-sequence-id-391815

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  19. pBetaActin-FKBP-Halo-Dync1h1motor

    Plasmid
    #191333
    Purpose
    Expresses the fluorescently labeled dynein motor domain fused to the dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB
    Depositor
    Frank Bradke
    Article
    Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:
    Insert
    FKBP-Halo-Dync1h1motor (Dync1h1 Mouse)
    Expression
    Mammalian
    Mutation
    DYNC1H1 motor domain aa 1453-4644
    Available Since
    March 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    191333-plasmid-map-sequence-id-381754

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  20. pBetaActin-FKBP-mScarlet-Dync1h1motorK2599T

    Plasmid
    #191331
    Purpose
    Expresses the fluorescently labeled motor-deficient dynein motor domain fused to the dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB
    Depositor
    Frank Bradke
    Article
    Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:
    Insert
    FKBP-mScarlet-Dync1h1motorK2599T (Dync1h1 Mouse)
    Expression
    Mammalian
    Mutation
    DYNC1H1 motor domain aa 1453-4644
    Available Since
    Jan. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    191331-plasmid-map-sequence-id-381751

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Showing: 7321 - 7340 of 7660 results