214,469 results

1. pCMV-PE7-P2A-hMLH1dn

   Plasmid

   #222997

   Purpose

   Mammalian expression of SpCas9 PE7 prime editor with P2A-human MLH1dn (codon optimized)

   Depositor

   Brittany Adamson

   Article

   Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

   Insert

   PE7-P2A-hMLH1dn
   

   Use

   Tags

   SV40 bpNLS and c-Myc NLS

   Expression

   Mammalian

   Mutation

   Deletion of residues 754-756 in MLH1 as described…

   Promoter

   CMV

   Available Since

   Aug. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti CMV Hygro DEST (w117-1)

   Plasmid

   #17454

   Purpose

   3rd gen lentiviral Gateway destination vector, expression, CMV promoter, Hygro

   Depositor

   Eric Campeau, Paul Kaufman

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Type

   Empty backbone

   Use

   Lentiviral; Destination vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti CMV Hygro DEST (w117-1)

   Plasmid

   #17454

   Purpose

   3rd gen lentiviral Gateway destination vector, expression, CMV promoter, Hygro

   Depositor

   Eric Campeau, Paul Kaufman

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Type

   Empty backbone

   Use

   Lentiviral; Destination vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. GW1-pHRed

   Plasmid

   #31473

   Purpose

   genetically encoded pH sensor made from mutagenesis of the red fluorescent protein mKeima

   Depositor

   Gary Yellen

   Article

   Tantama et al J Am Chem Soc. 2011 Jul 6. 133(26):10034

   Insert

   pHRed
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 21, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. GW1-pHRed

   Plasmid

   #31473

   Purpose

   genetically encoded pH sensor made from mutagenesis of the red fluorescent protein mKeima

   Depositor

   Gary Yellen

   Article

   Tantama et al J Am Chem Soc. 2011 Jul 6. 133(26):10034

   Insert

   pHRed
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 21, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEX6P1-mCherry-anti-mouse-IgG(kappa)-nanobody

   Plasmid

   #138130

   Purpose

   Expresses GST-tagged mCherry-anti-mouse-IgG(kappa)-nanobody in E. coli cells.

   Depositor

   Kazuhisa Nakayama

   Article

   Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

   Insert

   mCherry-anti-mouse-IgG(kappa)-nanobody
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. LC-E75

   Bacterial Strain

   #115925

   Purpose

   Expression of dCas9 gene under the control of a weak Ptet promoter in the E. coli chromosome

   Depositor

   David Bikard

   Article

   Cui et al Nat Commun. 2018 May 15;9(1):1912. doi:

   Bacterial Resistance

   None

   Available Since

   March 26, 2019

   Availability

   Academic Institutions and Nonprofits only

8. pJR89

   Plasmid

   #140096

   Purpose

   sgRNA constant region and hU6 insert for programmed dual sgRNA cloning. The sgRNA constant region contains a capture sequence (cs1) in the stem loop for direct capture Perturb-seq.

   Depositor

   Jonathan Weissman

   Article

   Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.

   Insert

   sgRNA constant region CR3 with cs1 in stem loop and hU6 promoter
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. PiggyBac PlayBack

   Plasmid

   #203312

   Purpose

   To all for PiggyBac gene insertion into Genome

   Depositor

   Aleksandar Necakov

   Article

   Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKM461

    Plasmid

    #108320

    Purpose

    For performing ORBIT - Expresses Che9c phage RecT and Bxb1 phage Integrase, and contains SacB

    Depositor

    Kenan Murphy

    Article

    Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

    Inserts

    Che9C RecT
    Bxb1 Integrase
    Tet Repressor
    SacRB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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