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pCDNA5/FRT/TO-ChemoL-NAD-NLS3x

Plasmid

#193828

Purpose

CMV overexpression of ChemoL-NAD in the nucleus of mammalian cell lines

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoL-NAD-NLS3x

Expression

Mammalian

Mutation

EGFP:A206K, T225R; HT7: L271E

Promoter

CMV

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/TO-[4xCox8]-ChemoL-NAD

Plasmid

#193829

Purpose

CMV overexpression of ChemoL-NAD in the mitochondria of mammalian cell lines

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

[4xCox8]-ChemoL-NAD

Expression

Mammalian

Mutation

EGFP:A206K, T225R; HT7: L271E

Promoter

CMV

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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KTK_346

Plasmid

#180549

Purpose

Entry vector containing gfasPurple

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

gfasPurple

Expression

Bacterial

Available Since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pgRNA_hMECP2_promoter_No8

Plasmid

#194902

Purpose

Lentiviral construct with mCherry and Puro cassettes to express sgRNA-8 targeting the promoter of human MECP2.

Depositor

Shawn Liu

Article

Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd

Insert

sgRNA targeting human MECP2 promoter No.8

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pEY91

Plasmid

#191084

Purpose

glr-4 fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

glr-4 (glr-4 Nematode)

Expression

Worm

Mutation

None

Available Since

Jan. 20, 2023

Availability

Academic Institutions and Nonprofits only

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CAGGS-AsCpf1-2A-GFP-U6-AAVS1-MAFB-sgRNA

Plasmid

#194725

Purpose

based on (CAGGS-AsCpf1-2A-GFP-U6-sgRNA-cloning vector, Addgene 159281), with guide RNA array targeting both AAVS1 and MAFB

Depositor

Rudolf Jaenisch

Article

Haiting et al bioRxiv

Insert

AsCpf1 (MAFB, AAVS1 Acidaminococcus sp.)

Use

CRISPR

Expression

Mammalian

Mutation

D908A

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pME-p3E-GLRX-roGFP2

Plasmid

#194288

Purpose

Multisite gateway entry clone for expression of the redox probe GLRX-roGFP2. Parton lab clone KQF

Depositor

Rob Parton

Article

Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

Insert

GLRX-roGFP2

Use

Multisite gateway entry clone

Available Since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pEY89

Plasmid

#191082

Purpose

glc-3 fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

glc-3 (glc-3 Nematode)

Expression

Worm

Mutation

None

Available Since

Nov. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pEY83

Plasmid

#191078

Purpose

flp-8 fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

flp-8 (flp-8 Nematode)

Expression

Worm

Mutation

None

Available Since

Nov. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pEY78

Plasmid

#191073

Purpose

dop-6 fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

dop-6 (dop-6 Nematode)

Expression

Worm

Mutation

None

Available Since

Nov. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pEY65

Plasmid

#191062

Purpose

bas-1 fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

bas-1 (bas-1 Nematode)

Expression

Worm

Mutation

None

Available Since

Nov. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST-pSP172BSSPE-Swa1::HA-RfA

Plasmid

#186404

Purpose

Destination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of N-ter HA tag and a gene of interest under control of a reg. sequence in the Swa1 restriction site.

Depositor

Patrick Lemaire

Article

Roure et al PLoS One. 2007. 2(9):e916.

Type

Empty backbone

Use

Gateway destination vector

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_H2B-STf

Plasmid

#181998

Purpose

CMV overexpression of SNAP-tag fast fusion to H2B for nuclear localization in mammalian cell lines

Depositor

Kai Johnsson

Article

Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

Insert

H2B-STf

Expression

Mammalian

Mutation

Fast mutation on SNAP-tag E30R

Promoter

CMV

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pEY86

Plasmid

#191079

Purpose

gcy-25 fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

gcy-25 (gcy-25 Nematode)

Expression

Worm

Mutation

None

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pEY87

Plasmid

#191080

Purpose

gcy-27 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

gcy-27 (gcy-27 Nematode)

Expression

Worm

Mutation

None

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pEY88

Plasmid

#191081

Purpose

ggr-1 fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

ggr-1

Expression

Worm

Mutation

None

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pEY90

Plasmid

#191083

Purpose

glr-2 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

glr-2 (glr-2 Nematode)

Expression

Worm

Mutation

None

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pEY92

Plasmid

#191085

Purpose

glr-8 fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

glr-8 (glr-8 Nematode)

Expression

Worm

Mutation

None

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pEY93

Plasmid

#191086

Purpose

gpa-11 fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

gpa-11 (gpa-11 Nematode)

Expression

Worm

Mutation

None

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pEY94

Plasmid

#191087

Purpose

gpa-13 fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

gpa-13 (gpa-13 Nematode)

Expression

Worm

Mutation

None

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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