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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 18,351 results for: multi

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Showing: 7341 - 7360 of 18351 results

  1. KG#908

    Plasmid
    #110914
    Purpose
    Expresses the plant proteins TIR1 (necessary for Auxin-Inducible Degradation) pan-neuronally in C. elegans
    Depositor
    Kenneth Miller
    Inserts
    rab-3 promoter
    TIR1 cDNA
    unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
    Expression
    Bacterial
    Available since
    March 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    110914-plasmid-map-sequence-id-212553

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  2. JI401: Ad5 E1A/Hexon qRT-PCR standard

    Plasmid
    #131753
    Purpose
    Plasmid standard containing regions of the Ad5 E1A and Ad5 Hexon genes to create a qRT-PCR standard curve for detection of replication competent adenovirus (RCA).
    Depositor
    Christopher Newgard
    Publication
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Inserts
    Ad5 E1A gene fragment
    Ad5 Hexon gene fragment
    Use
    Standard for qrt-pcr
    Promoter
    N/A
    Available since
    Oct. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    131753-plasmid-map-sequence-id-314394

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  5. pCarCBADE_ProB(I69E)A

    Plasmid
    #117756
    Purpose
    ProB(I69E)A and codon optimized CarCBADE enzymes (in that order) in pBbaA5k backbone
    Depositor
    Greg Bokinsky
    Publication
    Shomar et al Nat Chem Biol. 2018 Aug;14(8):794-800. d
    Insert
    Glutamate kinase (ProA), mutant pyrroline-5-carboxylate synthase (ProBI69E) from E. coli and codon optimised CarCBADE operon
    Expression
    Bacterial
    Promoter
    PLac(UV5)
    Available since
    Dec. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    117756-plasmid-map-sequence-id-230175

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  8. pDB036d

    Plasmid
    #99216
    Purpose
    Accessory plasmid for PACE of MjTyrRS variants; negative selection
    Depositor
    David Liu
    Publication
    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038
    Insert
    Ppsp [SD8] gIII; PproK tyrT(Opt,CUA); PproA [SD4] T7RNAP(S12*,S203*)
    Expression
    Bacterial
    Mutation
    amber codons at positions 12 and 203 of T7 RNA po…
    Promoter
    Ppsp, PproK, and PproA
    Available since
    Nov. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    99216-plasmid-map-sequence-id-198818

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  13. pLT 652

    Plasmid
    #59997
    Purpose
    Expresses a hybrid dsRNA corresponding to the Caenorhabditis elegans dhc-3/him-8 genes in bacteria; ingestion of the bacteria by C elegans produces an RNAi response & leads to more male progeny
    Depositor
    Lisa Timmons
    Publication
    Timmons et al FEBS Lett. 2014 Jul 24. pii: S0014-5793(
    Inserts
    dhc-3 (dhc-3 Nematode)
    him-8 (him-8 Nematode)
    Expression
    Bacterial
    Mutation
    partial genomic
    Available since
    April 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    59997-plasmid-map-sequence-id-110770

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  15. pGLB2 5'3'

    Plasmid
    #49346
    Purpose
    luciferase reporter with Bmp2 promoter (1702 bp) and Bmp2 3'UTR (2015 bp)
    Depositor
    Melissa Rogers
    Publication
    Fritz et al J Biol Chem. 2004 Nov 19;279(47):48950-8
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Bmp2 distal promoter (Bmp2 Mouse)
    Bmp2 3' UTR (Bmp2 Mouse)
    Use
    Luciferase
    Tags
    luciferase
    Promoter
    -
    Available since
    Dec. 9, 2013
    Availability
    Academic Institutions and Nonprofits only

  18. F2R-DuET

    Plasmid
    #213234
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Publication
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    F2R (F2R Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213234-plasmid-map-sequence-id-430897

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  19. pWZ852

    Plasmid
    #208114
    Purpose
    mSwAP-In marker cassette 1 with mScarlet-HygTK
    Depositor
    Jef Boeke
    Publication
    Zhang et al Nature. 2023 Nov 1. doi: 10.1038/s41586-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mScarlet-HygTK
    Tags
    T2A
    Expression
    Bacterial and Mammalian
    Promoter
    EF1alpha
    Available since
    Nov. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    208114-plasmid-map-sequence-id-413317

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  20. pcDNA5/FRT/TO_IgKchL_HA_STf_myc_PDGFRtmb

    Plasmid
    #182009
    Purpose
    CMV overexpression of SNAP-tag fast at the outer leaflet of the plasma membrane of mammalian cell lines
    Depositor
    Kai Johnsson
    Publication
    Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    IgKchL_HA_STf_myc_PDGFRtmb
    Expression
    Mammalian
    Mutation
    Fast mutation on SNAP-tag E30R
    Promoter
    CMV
    Available since
    Nov. 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    182009-plasmid-map-sequence-id-355921

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Showing: 7341 - 7360 of 18351 results