We narrowed to 118,601 results for: MPI
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Plasmid#140235PurposeF3 (FRT variant)-flanked pPgk-pEM7-Neo*-bpA positive selection cassetteDepositorInsertNeo*
UseMouse Targeting; Flp/ frtExpressionBacterial and MammalianAvailable SinceNov. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLIX403-EBFP2-ccdB-EYFP
Plasmid#158558PurposeSet of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusionsDepositorTypeEmpty backboneUseLentiviralTagsEBFP2ExpressionMammalianAvailable SinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-HisFlag-rUSP19CYT
Plasmid#155238Purposefor mammalian overexpression of the cytoplasmic isoform of rat USP19DepositorInsertUSP19 (Cytoplasmic isoform) (Usp19 Rat, Mouse)
Tags6XHis-3XFlagExpressionMammalianPromoterCMVAvailable SinceSept. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZNF302-donor
Plasmid#132513PurposeCRISPR donor plasmid to create GFP fusion proteinsDepositorAvailable SinceJuly 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBABEneo mt T Ag
Plasmid#133713PurposeLarge T antigen mutant form in pBABEneo vector for mammalian expression.DepositorInsertSV40gp6 (SV40gp6 SV40)
UseRetroviralMutationaa 1-354 of T antigen (including aa 1-130); no p5…Available SinceMarch 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB CAG-R-bpA-3xSV40pA-R-td-sfGFP
Plasmid#133579PurposeCAG tdGFP dre/rox reporter piggyBac transposon (bpA 3x SV40pA stop)DepositorInsertTandem dimer superfolder GFP
UseDre/roxExpressionMammalianAvailable SinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPATZ1.1.0-gDNA
Plasmid#132476PurposeCRISPR/Cas9 plasmid to create GFP fusion proteinsDepositorInsertPATZ1 (PATZ1 Human)
UseCRISPRAvailable SinceDec. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRARB.1.0-gDNA
Plasmid#132471PurposeCRISPR/Cas9 plasmid to create GFP fusion proteinsDepositorInsertRARB (RARB Human)
UseCRISPRAvailable SinceDec. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZNF302.1.0-gDNA
Plasmid#132467PurposeCRISPR/Cas9 plasmid to create GFP fusion proteinsDepositorInsertZNF302 (ZNF302 Human)
UseCRISPRAvailable SinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZFPM2.1.0-gDNA
Plasmid#132459PurposeCRISPR/Cas9 plasmid to create GFP fusion proteinsDepositorInsertZFPM2 (ZFPM2 Human)
UseCRISPRAvailable SinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
UC16m
Plasmid#121038PurposeMoClo golden gate assembly DE part for gQi gRNA (guide RNA for S. pyogenes Cas9; sequence from DOI: 10.1016/j.cell.2013.02.022). Please see Supplemental Documents for annotated Genbank file.DepositorInsertgQi Cas9 gRNA UC part
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
UC17m
Plasmid#121039PurposeMoClo golden gate assembly DE part for gV1 gRNA (guide RNA for S. pyogenes Cas9; sequence from doi: [10.15252/msb.20145735]). Please see Supplemental Documents for annotated Genbank file.DepositorInsertgV1 Cas9 gRNA UC part
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C105m
Plasmid#121008PurposeMoClo golden gate assembly CD part for sfYFP-AAV (superfolder YFP with AAV degradation tag for ssrA). Please see Supplemental Documents for annotated Genbank file.DepositorInsertsfYFP-AAV ssrA (has bbsI)
UseSynthetic BiologyTagsAAV degradation tag for ssrAAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C106m
Plasmid#121009PurposeMoClo golden gate assembly CD part for sfYFP-DAS (superfolder YFP with DAS degradation tag for ssrA). Please see Supplemental Documents for annotated Genbank file.DepositorInsertsfYFP-DAS ssrA (has bbsI)
UseSynthetic BiologyTagsDAS degradation tag for ssrAAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C108m
Plasmid#121010PurposeMoClo golden gate assembly CD part for mScarlet-I-pdt3 (fast-folding mScarlet variant, codon-optimized for E. col, with pdt3 degradation tag; see https://www.fpbase.org/protein/mscarlet-i/).DepositorInsertmScarlet-I codon optimized with pdt3 tag
UseSynthetic BiologyTagspdt3 mfLon degradation tagAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C109m
Plasmid#121011PurposeMoClo golden gate assembly CD part for mfLon-pdt3 (mfLon protease with self-targeting degradation tag pdt3). Please see Supplemental Documents for annotated Genbank file.DepositorInsertmfLon protease with pdt3 tag
UseSynthetic BiologyTagspdt3 mfLon degradation tagAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C103m
Plasmid#121007PurposeMoClo golden gate assembly CD part for mScarlet-I-LAA (fast-folding mScarlet variant, codon-optimized for E. col, with LAA degradation tag; see https://www.fpbase.org/protein/mscarlet-i/).DepositorInsertmScarlet-I codon optimized with LAA tag
UseSynthetic BiologyTagsLAA degradation tag for ssrAAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
P61m
Plasmid#120953PurposeMoClo golden gate assembly AB part for pTac (Adam Meyer's improved pTac promoter selected for dynamic range and low cross-sensitivity; unpublished, using strategy from https://doi.org/10.1101/285866).DepositorInsertTac promoter Adam Meyer
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
P35m
Plasmid#120946PurposeMoClo golden gate assembly AB part for pLbda-CasRQi (strong lambda promoter with downstream dCas binding site for Qi-lab gRNA (UC16m); gRNA sequence taken from DOI: 10.1016/j.cell.2013.02.022).DepositorInsertpLbda-CasRQi
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-Blast_HP1beta_gRNA
Plasmid#127908PurposeWT Cas9 Vector with Blasticidin Selection Marker targeting the 5' end of the human HP1beta geneDepositorInsertgRNA for Human 5' HP1beta
UseCRISPRAvailable SinceSept. 12, 2019AvailabilityAcademic Institutions and Nonprofits only