We narrowed to 18,595 results for: MUT

1. pPB9P3

   Plasmid

   #210034

   Purpose

   Contains cloning site for PE3/3b prime editing gRNA constructs, inducible prime editor 2, rtTA-T2A-puro

   Depositor

   Michael Talkowski

   Article

   Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

   Inserts

   prime editor 2
   rtta-T2A-puro
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Mammalian

   Promoter

   EF-1α and TRE3G

   Available Since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHSP90(G97D)-mOFP

   Plasmid

   #228195

   Purpose

   expressed human Hsp90 (G97D mutation) with a mOFP fluorescent protein tag

   Depositor

   Stephen Benkovic

   Article

   French et al Proc Natl Acad Sci U S A. 2013 Feb 12;11

   Insert

   HSP90AA1(G97D) (HSP90AA1 Human)
   

   Tags

   mOFP

   Expression

   Mammalian

   Mutation

   G97D mutation

   Promoter

   CMV

   Available Since

   Dec. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. eft-3p:act-5(ptc):tbb2 3' UTR

   Plasmid

   #202331

   Purpose

   The ubiquitous eft-3 promoter drives overexpression of the mutant act-5(ptc) gene including introns.

   Depositor

   Didier Stainier

   Article

   Welker et al PLoS Genet. 2023 Jun 29;19(6):e1010806.

   Inserts

   eft-3 promoter
   act-5(ptc)
   tbb-2 3' UTR
   

   Tags

   base 238 C -> A mutation causing a PTC at codo…

   Expression

   Worm

   Promoter

   This is a promoter sequence for C. elegans eft-3,…

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEX6P1-NEDD4L_FL-CS

   Plasmid

   #187713

   Purpose

   Express the catalytically inactive GST-tagged NEDD4L FL in bacterial cells

   Depositor

   Sandra Gabelli

   Article

   Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

   Insert

   NEDD4L FL with C874 mutated to a serine (NEDD4L Human)
   

   Tags

   GST-HRV 3C site

   Expression

   Bacterial

   Mutation

   Cys874 mutated to a serine

   Available Since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcs2-Δhel1/Δhel2HRI-GFP-IRES-mCherry

   Plasmid

   #226101

   Purpose

   HRI stability reporter construct with deletion of SIFI degron helices 1&2 for transient expression in mammalian cells

   Depositor

   Michael Rape

   Article

   Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:

   Insert

   HRI (EIF2AK1 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   SIFI degron helices 1 & 2 deleted (Δ61-112)

   Available Since

   Oct. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG_SRGAP2A_SH3(W765A)_GFP

   Plasmid

   #222616

   Purpose

   Expresses a form of full length SRGAP2 with one point mutation (W765A) in the SH3 domain abrogating its ability to bind to Gephyrin.

   Depositor

   Franck Polleux

   Article

   Fossati et al Neuron. 2016 Jul 20;91(2):356-69. doi: 1

   Insert

   SRGAP2 (SRGAP2 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Point mutation W765A (SH3 domain)

   Available Since

   Oct. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG_SRGAP2A_SH3(W765A)_HA

   Plasmid

   #222614

   Purpose

   Expresses a form of full length SRGAP2 with one point mutation (W765A) in the SH3 domain abrogating its ability to bind to Gephyrin.

   Depositor

   Franck Polleux

   Article

   Fossati et al Neuron. 2016 Jul 20;91(2):356-69. doi: 1

   Insert

   SRGAP2 (SRGAP2 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Point mutation W765A (SH3 domain)

   Available Since

   Oct. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG SRGAP2A_EVH1(P339L F342C)

   Plasmid

   #222613

   Purpose

   Expresses a form of full length SRGAP2 with two point mutations (P339L and F342C) that abrogate EVH1 binding site for Homer1

   Depositor

   Franck Polleux

   Article

   Fossati et al Neuron. 2016 Jul 20;91(2):356-69. doi: 1

   Insert

   SRGAP2 (SRGAP2 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Point mutations P339L and F342C

   Available Since

   Oct. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDNA3-Regnase-1 D141N

   Plasmid

   #222654

   Purpose

   Mammalian expression vector to express mouse Regnase-1 D141N mutant

   Depositor

   Silvia Monticelli

   Article

   Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

   Insert

   Zc3h12a (Zc3h12a Mouse)
   

   Expression

   Mammalian

   Mutation

   mutated aspartic acid 141 to asparagine to abroga…

   Promoter

   CMV

   Available Since

   Oct. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUC57 mini-FLAG-HA-Regnase-3 D252N

    Plasmid

    #222652

    Purpose

    For in vitro transcription of mouse Zc3h12c D252N mutant tagged with FLAG-HA at N-term

    Depositor

    Silvia Monticelli

    Article

    Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

    Insert

    Zc3h12c (Zc3h12c Mouse)
    

    Use

    In vitro transcription

    Tags

    FLAG and HA

    Mutation

    mutated aspartic acid 252 to asparagine to abroga…

    Promoter

    T7

    Available Since

    Oct. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcs2-N-HRI(1-138)-GFP-IRES-mCherry

    Plasmid

    #226100

    Purpose

    HRI N-terminal region (residues 1-138) stability reporter construct for transient expression in mammalian cells

    Depositor

    Michael Rape

    Article

    Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:

    Insert

    HRI (EIF2AK1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    contains only N-HRI (aa 1-138)

    Available Since

    Oct. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. GFP-PODXL FBM*/PBM*

    Plasmid

    #195221

    Purpose

    Mammalian expression vector containing GFP tagged Podocalyxin. Dual mutant for HRS>AAA / lacking DTHL.

    Depositor

    David Bryant, Keith Mostov

    Article

    Bryant et al Dev Cell. 2014 Oct 27;31(2):171-87. doi:

    Insert

    PODXL (PODXL Rabbit)
    

    Expression

    Mammalian

    Mutation

    Dual mutant for HRS>AAA / lacking DTHL

    Available Since

    Sept. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pACB120

    Plasmid

    #222213

    Purpose

    For recombinant expression of fghA (PP_1617 from Pseudomonas putida) with five mutations (K10N, S11R, W15C, G76V, G258D) and a C-terminal His tag

    Depositor

    Gregg Beckham

    Article

    Bleem et al Metab Eng. 2024 Jul;84:145-157. doi: 10.

    Insert

    fghA(K10N, S11R, W15C, G76V, G258D)
    

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Contains five amino acid mutations: K10N, S11R, W…

    Promoter

    T7

    Available Since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. lentiGuide-puro_Panc480-MT7

    Plasmid

    #200941

    Purpose

    Multiplexed CRISPR array expressing 7 sgRNA in a lentiviral backbone

    Depositor

    James Eshleman

    Article

    Teh et al NAR Cancer. 2024 Jun 19;6(2):zcae028. do

    Insert

    multiplexed sgRNA array
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6, EF-1a

    Available Since

    July 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRRL-VcnTS-E1015A-E1021A

    Plasmid

    #213411

    Purpose

    Vinculin tension sensor (VcnTS) with both directionally asymmetric, force strengthening (DAFS) variant point mutations (E1015A and E1021A), in lentiviral expression vector.

    Depositor

    Brenton Hoffman

    Article

    Chirasani et al Nat Commun. 2023 Dec 14;14(1):8300. doi:

    Insert

    VcnTS-E1015A-E1021A (VCL Synthetic, Chicken)
    

    Use

    Lentiviral

    Mutation

    Mutated vinculin glutamic acid 1015 and 1021 to a…

    Promoter

    CMV

    Available Since

    Feb. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pENTR-Creb5(T59/T61A)(HA)

    Plasmid

    #195025

    Purpose

    Gateway vector containing HA-tagged Creb5 with T59/T61A mutation

    Depositor

    Andrew Lassar, Cheng-Hai Zhang

    Article

    Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

    Insert

    HA-tagged Creb5 (full length, 508aa; with with T59/T61A mutation) (CREB5 Bovine)
    

    Use

    Gateway cloning

    Tags

    3xHA

    Mutation

    T59/T61A

    Available Since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pENTR-Creb5(T59/T61D)(HA)

    Plasmid

    #195026

    Purpose

    Gateway vector containing HA-tagged Creb5 with T59/T61D mutation

    Depositor

    Andrew Lassar, Cheng-Hai Zhang

    Article

    Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

    Insert

    HA-tagged Creb5 (full length, 508aa; with with T59/T61D mutation) (CREB5 Bovine)
    

    Use

    Gateway cloning

    Tags

    3xHA

    Mutation

    T59/T61D

    Available Since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. SpyTag003(M115G)-Talin rod-mCherry

    Plasmid

    #210026

    Purpose

    Expression of SpyTag003(M115G)-Talin rod(434-2541)-mCherry in mammalian cells

    Depositor

    Vesa Hytönen

    Article

    Rahikainen et al J Am Chem Soc. 2023 Nov 15;145(45):24459

    Insert

    SpyTag003(M115G)-Talin1 rod domain(434-2541)-mCherry
    

    Expression

    Mammalian

    Mutation

    SpyTag003 M115G mutation to modify SpyCatcher003 …

    Promoter

    CMV

    Available Since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. SpyTag003(M115A)-Talin rod-mCherry

    Plasmid

    #210025

    Purpose

    Expression of SpyTag003(M115A)-Talin rod(434-2541)-mCherry in mammalian cells

    Depositor

    Vesa Hytönen

    Article

    Rahikainen et al J Am Chem Soc. 2023 Nov 15;145(45):24459

    Insert

    SpyTag003(M115A)-Talin1 rod domain(434-2541)-mCherry
    

    Expression

    Mammalian

    Mutation

    SpyTag003 M115A mutation to modify SpyCatcher003 …

    Available Since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. SpyTag003(V114T, V116T)-Talin rod-mCherry

    Plasmid

    #210027

    Purpose

    Expression of SpyTag003(V114T, V116T)-Talin rod(434-2541)-mCherry in mammalian cells

    Depositor

    Vesa Hytönen

    Article

    Rahikainen et al J Am Chem Soc. 2023 Nov 15;145(45):24459

    Insert

    SpyTag003(V114T,V116T)-Talin1 rod domain(434-2541)-mCherry
    

    Expression

    Mammalian

    Mutation

    SpyTag003 V114T and V116T mutations to modify Spy…

    Promoter

    CMV

    Available Since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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