Addgene

We narrowed to 19,228 results for: KIN

Showing: 721 - 740 of 19228 results

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CMV PGK1-3xHA

Plasmid

#220913

Purpose

Expresses PGK1-3xHA

Depositor

Timothy Ryan

Article

Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

Insert

PGK1-3xHA (PGK1 Human)

Use

Affinity Reagent/ Antibody

Tags

3xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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IKK-2 K44M

Plasmid

#11104

Depositor

Anjana Rao

Article

Mercurio et al Science. 1997 Oct 31. 278(5339):860-6.

Insert

IKK-2 (IKBKB Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

K44M, kinase inactive

Promoter

Available since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

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pET28a-TEV-TBP6.9F34MF37MF77M

Plasmid

#168268

Purpose

Expresses human TBP6.9 with the F34M/F37M/F77M triple mutant

Depositor

Joseph Wedekind

Article

Srivastava et al Crystals (Basel). 2021 Mar;11(3). doi: 1

Insert

TBP6.9

Use

Tags

6xHis tag N-terminus, TEV protease cleavage site

Expression

Bacterial

Mutation

changed F34M, F37M and F77M

Promoter

T7 promotor

Available since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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pBV-Luc wt MBS1-4

Plasmid

#16564

Depositor

Bert Vogelstein

Article

Hermeking et al Proc Natl Acad Sci U S A. 2000 Feb 29. 9

Insert

c-MYC binding sites (MYC Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Promoter

Available since

March 24, 2008

Availability

Academic Institutions and Nonprofits only

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RS35075_pMQ30_KO_Kan

Plasmid

#185390

Purpose

Non-replicating vector used to create markerless deletion of RR42_RS35075 in C. basilensis 4G11

Depositor

Adam Arkin

Article

Sander et al Biotechnol Bioeng. 2024 Jan;121(1):139-1

Inserts

RR42_RS35075 upstream flanking homology region
RR42_RS35075 downstream flanking homology region
nptII kanamycin resistance marker

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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PERK 5: PERK.K618R.pGEX4T-1

Plasmid

#21818

Depositor

David Ron

Article

Harding et al Nature. 1999 Jan 21. 397(6716):271-4.

Insert

PERK (537-1114) K618R (Eif2ak3 Mouse)

Use

Tags

GST and Myc (9E10)

Expression

Bacterial

Mutation

PERK Kinase Domain. Amino acids 537-1114. Kinase …

Promoter

Available since

March 29, 2010

Availability

Academic Institutions and Nonprofits only

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2XMyc-LRRK2-3XKD

Plasmid

#25366

Depositor

Mark Cookson

Article

J Biol Chem. 2008 Jun 13;283(24):16906-1

Insert

LRRK2 (LRRK2 Human)

Use

Tags

2XMyc

Expression

Mammalian

Mutation

"Kinase Dead" Lysine 1906 mutated to A…

Promoter

Available since

June 29, 2010

Availability

Academic Institutions and Nonprofits only

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AAV hSyn hPGK1-mRuby

Plasmid

#220911

Purpose

Neuronal specific expression of the fusion construct PGK1-mRuby

Depositor

Timothy Ryan

Article

Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

Insert

PGK1-mRuby (PGK1 Human)

Use

AAV

Tags

mRuby

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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pBV-Luc mut MBS1-4

Plasmid

#16565

Depositor

Bert Vogelstein

Article

Hermeking et al Proc Natl Acad Sci U S A. 2000 Feb 29. 9

Insert

c-MYC binding sites (MYC Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Mutant MBS1-4

Promoter

Available since

March 24, 2008

Availability

Academic Institutions and Nonprofits only
plenti hSyn PGK1-HALO

Plasmid

#220910

Purpose

Neuronal specific expression of the fusion construct PGK1-HALO

Depositor

Timothy Ryan

Article

Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

Insert

PGK1-HALO (PGK1 Human)

Use

Lentiviral

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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pEBTet-SNAP-CCCAP

Plasmid

#136798

Purpose

Mammalian expression of the centrosomal protein CCCAP N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-CCCAP (SDCCAG8 Synthetic, Human)

Use

Tags

FLAG-tag / His-tag

Expression

Mammalian

Mutation

Promoter

CMV-TetO2

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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H9 pMalLP

Plasmid

#41811

Depositor

Chris Garcia

Article

Levin et al Nature. 2012 Mar 25;484(7395):529-33. do

Insert

H9 Super-2 (IL2 Human)

Use

Tags

3C protease site, 6x His, and Maltose Binding Pro…

Expression

Bacterial

Mutation

Promoter

pTac

Available since

Jan. 8, 2013

Availability

Academic Institutions and Nonprofits only
pEBTet-SNAP-Sfi1

Plasmid

#136793

Purpose

Mammalian expression of the centrosomal protein hSfi1 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-hSfi1 (SFI1 Synthetic, Human)

Use

Tags

FLAG-tag / His-tag

Expression

Mammalian

Mutation

Promoter

CMV-TetO2

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pEBTet-SNAP-plk4

Plasmid

#136788

Purpose

Mammalian expression of the centrosomal protein plk4 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-plk4 (PLK4 Synthetic, Human)

Use

Tags

FLAG-tag / His-tag

Expression

Mammalian

Mutation

Promoter

CMV-TetO2

Available since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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pKM294

Plasmid

#134179

Purpose

Mammalian expression of CENP-C and KNL2

Depositor

Iain Cheeseman

Article

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

mcherry-CENP-C 510-end-KNL2 (CENPC Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pKM296

Plasmid

#134180

Purpose

Mammalian expression of CENP-C and KNL2 CDK SA

Depositor

Iain Cheeseman

Article

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

mcherry-CENP-C 510-end-KNL2 CDK SA (CENPC Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pMSCV-KD-mAbl

Plasmid

#69028

Purpose

Retorviral vector encoding Kinase Defective mouse c-Abl

Depositor

Jean Wang

Article

Sridevi et al PLoS One. 2013 Oct 11;8(10):e77495. doi:

Insert

mouse c-Abl Type VI - kinase defective

Use

Retroviral

Tags

FLAG tag inserted at Internal SalI site (2934 bp)

Expression

Mammalian

Mutation

The KD (kinase defective) mutation is at Lysine 2…

Promoter

Available since

Sept. 2, 2015

Availability

Academic Institutions and Nonprofits only
pEBTet-SNAP-HsSAS6

Plasmid

#136789

Purpose

Mammalian expression of the centrosomal protein SAS6 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-SAS6 (SASS6 Synthetic, Human)

Use

Tags

FLAG-tag / His-tag

Expression

Mammalian

Mutation

Promoter

CMV-TetO2

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5 FRT/TO Plk4 KD ∆24-mCherry

Plasmid

#80272

Purpose

mammalian expression plasmid for kinase dead Plk4 with multi-phospho degron deleted

Depositor

Andrew Holland

Article

Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

Insert

Plk4 (PLK4 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

deletion of multi-phospho degron (amino acids 282…

Promoter

CMV

Available since

Aug. 2, 2016

Availability

Academic Institutions and Nonprofits only

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2XMyc-LRRK2-RCK-Y1699C

Plasmid

#25067

Depositor

Mark Cookson

Article

J Biol Chem. 2008 Jun 13;283(24):16906-1

Insert

LRRK2 (LRRK2 Human)

Use

Tags

2XMyc

Expression

Mammalian

Mutation

Contains only the ROC-COR-Kinase domains aa 1329…

Promoter

Available since

June 29, 2010

Availability

Academic Institutions and Nonprofits only