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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,038 results for: biotin

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Showing: 721 - 740 of 1038 results

  1. pET22b-EC12WTC9AAvi

    Plasmid
    #47925
    Purpose
    Expresses E-cadherin EC12 WT with C9A mutation and avi tag in E.Coli.
    Depositor
    Tanja Kortemme
    Publication
    Ritterson et al J Am Chem Soc. 2013 Aug 7.
    Insert
    E-Cadherin EC12
    Tags
    6xHis, Avi Tag, and TEV Cleavage
    Expression
    Bacterial
    Mutation
    changed Cysteine 9 to Alanine
    Promoter
    T7
    Available since
    Sept. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    47925-plasmid-map-sequence-id-68712

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  4. pcDNA4/TO-bio-myc-NES-EGFP

    Plasmid
    #112712
    Purpose
    For tetracycline-inducible expression of bio-myc-NES-EGFP in mammalian cells
    Depositor
    Daniel Schoenberg
    Publication
    Trotman et al J Biol Chem. 2018 Oct 26;293(43):16596-1
    Insert
    EGFP
    Tags
    HIV Rev nuclear localization signal (NES), biotin…
    Expression
    Mammalian
    Mutation
    Nucleotide sequence optimized for synthetic gene …
    Promoter
    CMV with Tet repressor binding sites
    Available since
    May 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    112712-plasmid-map-sequence-id-217058

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  5. PF3D7_1431400-bio

    Plasmid
    #47791
    Purpose
    Expresses enzymatically monobiotinylated full-length PF3D7_1431400 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Publication
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Insert
    Codon-optimised PF3D7_1431400
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    47791-plasmid-map-sequence-id-68871

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  6. TLP-bio

    Plasmid
    #47787
    Purpose
    Expresses enzymatically monobiotinylated full-length TLP ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Publication
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Insert
    Codon-optimised TLP
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    47787-plasmid-map-sequence-id-68823

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  9. pTCF170 H-2L(d)

    Plasmid
    #180458
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    John Altman
    Insert
    H-2L(d)
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    Replacement of transmembrane domain with enzymati…
    Available since
    March 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    180458-plasmid-map-sequence-id-355847

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  16. pTCF79 H-2D(b) (CD8 mut)

    Plasmid
    #180453
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    John Altman
    Insert
    H-2D(b) (CD8 mut)
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    D227K, replacement of transmembrane domain with e…
    Available since
    Sept. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    180453-plasmid-map-sequence-id-409097

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  18. p663-UBC-PM-miniTurbo-V5_IDG-K

    Plasmid
    #214454
    Purpose
    Gateway destination clone of PM-miniTurbo (plasma membrane control) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    PM-miniTurbo-V5
    Use
    Lentiviral; Gateway destination
    Tags
    V5
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available since
    March 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    214454-plasmid-map-sequence-id-433841

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Showing: 721 - 740 of 1038 results