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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,041 results for: biotin

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Showing: 721 - 740 of 1041 results

  1. pDS170-enNTS1

    Plasmid
    #199152
    Purpose
    JZ73, bacterial expression construct expressing thermostabilised rat neurotensin receptor 1 (enNTS1)
    Depositor
    Daniel Scott
    Publication
    Bumbak et al Cell Rep. 2023 Jan 31;42(1):112015. doi:
    Insert
    enNTS1 (Ntsr1 Rat)
    Tags
    10xHis tag, Avi tag, MBP, and muGFP
    Expression
    Bacterial
    Mutation
    V208M, C273S, C391S and C393S (over NTS1-B5)
    Promoter
    Lac/T5
    Available since
    May 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    199152-plasmid-map-sequence-id-393468

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  2. PRDX6A-c001

    Plasmid
    #175474
    Purpose
    Protein expression in bacterial cells. Entire CDS, M1-P224. Can be biotinylated.
    Depositor
    Opher Gileadi
    Insert
    PRDX6 (PRDX6 Human)
    Tags
    AviTag and His6-TEV
    Expression
    Bacterial
    Mutation
    See Depositor Comments
    Available since
    Feb. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    175474-plasmid-map-sequence-id-353175

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  4. pET22b-EC12WTC9AAvi

    Plasmid
    #47925
    Purpose
    Expresses E-cadherin EC12 WT with C9A mutation and avi tag in E.Coli.
    Depositor
    Tanja Kortemme
    Publication
    Ritterson et al J Am Chem Soc. 2013 Aug 7.
    Insert
    E-Cadherin EC12
    Tags
    6xHis, Avi Tag, and TEV Cleavage
    Expression
    Bacterial
    Mutation
    changed Cysteine 9 to Alanine
    Promoter
    T7
    Available since
    Sept. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    47925-plasmid-map-sequence-id-68712

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  7. pcDNA4/TO-bio-myc-NES-EGFP

    Plasmid
    #112712
    Purpose
    For tetracycline-inducible expression of bio-myc-NES-EGFP in mammalian cells
    Depositor
    Daniel Schoenberg
    Publication
    Trotman et al J Biol Chem. 2018 Oct 26;293(43):16596-1
    Insert
    EGFP
    Tags
    HIV Rev nuclear localization signal (NES), biotin…
    Expression
    Mammalian
    Mutation
    Nucleotide sequence optimized for synthetic gene …
    Promoter
    CMV with Tet repressor binding sites
    Available since
    May 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    112712-plasmid-map-sequence-id-217058

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  8. PF3D7_1431400-bio

    Plasmid
    #47791
    Purpose
    Expresses enzymatically monobiotinylated full-length PF3D7_1431400 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Publication
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Insert
    Codon-optimised PF3D7_1431400
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    47791-plasmid-map-sequence-id-68871

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  9. TLP-bio

    Plasmid
    #47787
    Purpose
    Expresses enzymatically monobiotinylated full-length TLP ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Publication
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Insert
    Codon-optimised TLP
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    47787-plasmid-map-sequence-id-68823

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  10. pTCF170 H-2L(d)

    Plasmid
    #180458
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    John Altman
    Insert
    H-2L(d)
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    Replacement of transmembrane domain with enzymati…
    Available since
    March 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    180458-plasmid-map-sequence-id-355847

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  13. JB97

    Plasmid
    #128926
    Purpose
    mammalian expression, proximity labelling, mitochondrial relocalisation
    Depositor
    Sean Munro
    Publication
    Gillingham et al Elife. 2019 Jul 11;8. pii: 45916. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RalBGV-BirA*-HA-MAO (RALB Human)
    Expression
    Mammalian
    Available since
    Aug. 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    128926-plasmid-map-sequence-id-254245

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  14. JB3

    Plasmid
    #128919
    Purpose
    mammalian expression, proximity labelling, mitochondrial relocalisation
    Depositor
    Sean Munro
    Publication
    Gillingham et al Elife. 2019 Jul 11;8. pii: 45916. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Rab30TN-BirA*-HA-MAO (RAB30 Human)
    Expression
    Mammalian
    Available since
    Aug. 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    128919-plasmid-map-sequence-id-254223

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  15. pTCF79 H-2D(b) (CD8 mut)

    Plasmid
    #180453
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    John Altman
    Insert
    H-2D(b) (CD8 mut)
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    D227K, replacement of transmembrane domain with e…
    Available since
    Sept. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    180453-plasmid-map-sequence-id-409097

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  17. JB24

    Plasmid
    #128906
    Purpose
    mammalian expression, proximity labelling, mitochondrial relocalisation
    Depositor
    Sean Munro
    Publication
    Gillingham et al Elife. 2019 Jul 11;8. pii: 45916. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Rab8AQL-BirA*-HA-MAO (RAB8A Human)
    Expression
    Mammalian
    Available since
    Aug. 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    128906-plasmid-map-sequence-id-254234

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  18. JB10

    Plasmid
    #128917
    Purpose
    mammalian expression, proximity labelling, mitochondrial relocalisation
    Depositor
    Sean Munro
    Publication
    Gillingham et al Elife. 2019 Jul 11;8. pii: 45916. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Rab18SN-BirA*-HA-MAO (RAB18 Human)
    Expression
    Mammalian
    Available since
    Aug. 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    128917-plasmid-map-sequence-id-254219

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  19. JB2

    Plasmid
    #128899
    Purpose
    mammalian expression, proximity labelling, mitochondrial relocalisation
    Depositor
    Sean Munro
    Publication
    Gillingham et al Elife. 2019 Jul 11;8. pii: 45916. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Rab2ASN-BirA*-HA-MAO (RAB2A Human)
    Expression
    Mammalian
    Available since
    Aug. 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    128899-plasmid-map-sequence-id-254204

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  20. p663-UBC-PM-miniTurbo-V5_IDG-K

    Plasmid
    #214454
    Purpose
    Gateway destination clone of PM-miniTurbo (plasma membrane control) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    PM-miniTurbo-V5
    Use
    Lentiviral; Gateway destination
    Tags
    V5
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available since
    March 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    214454-plasmid-map-sequence-id-433841

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Showing: 721 - 740 of 1041 results