Addgene

We narrowed to 16,731 results for: emb

Showing: 721 - 740 of 16731 results

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BASIC_8_RBS34- mCherry -B0015

Plasmid

#68142

Purpose

storage vector for BASIC part: RBS-RFP(ORF)

Depositor

Geoffrey Baldwin

Article

Storch et al ACS Synth Biol. 2015 Apr 16.

Insert

RBS34-mcherry-B0015

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 28, 2015

Availability

Academic Institutions and Nonprofits only

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Aahn

Plasmid

#112158

Purpose

A genetically encoded voltage indicator (Aahn), capable of optically monitoring both plasma membrane and internal membrane potentials.

Depositor

Bradley Baker

Article

Sepehri Rad et al Sci Rep. 2018 May 2;8(1):6911. doi: 10.1

Insert

Aahn

Use

Tags

Super ecliptic pHlorin A227D

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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BASIC_7_J23101-RBS34-mCherry-B0015

Plasmid

#68141

Purpose

storage vector for BASIC part: RFP expression cassette

Depositor

Geoffrey Baldwin

Article

Storch et al ACS Synth Biol. 2015 Apr 16.

Insert

J23101-RBS34-mcherry-B0015

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pSA10(gfcD)

Plasmid

#176191

Purpose

gfcD protein expression

Depositor

Mark Saper

Article

Larson et al PLoS One. 2021 Nov 15;16(11):e0259900. d

Insert

gfcD

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Ptac

Available since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pSA10(gfcD-10xHis)

Plasmid

#176192

Purpose

gfcD protein expression

Depositor

Mark Saper

Article

Larson et al PLoS One. 2021 Nov 15;16(11):e0259900. d

Insert

gfcD

Use

Tags

10xHis

Expression

Bacterial

Mutation

Promoter

Ptac

Available since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pMCSG26(gfcD)

Plasmid

#176198

Purpose

gfcD protein expression

Depositor

Mark Saper

Article

Larson et al PLoS One. 2021 Nov 15;16(11):e0259900. d

Insert

gfcD

Use

Tags

10xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pSA10(gfcBCD)

Plasmid

#176193

Purpose

expresses 3 overlapping genes (gfcB, gfcC, gfcD) from this one operon

Depositor

Mark Saper

Article

Larson et al PLoS One. 2021 Nov 15;16(11):e0259900. d

Insert

gfcBCD

Use

Tags

Expression

Bacterial

Mutation

Promoter

Ptac

Available since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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Golgi-DKAR

Plasmid

#49790

Purpose

FRET-based, Golgi-targeted kinase activity reporter for PKD

Depositor

Alexandra Newton

Article

Kunkel et al J Biol Chem. 2010 Jul 23;285(30):22748-5

Insert

D kinase activity reporter

Use

Tags

CFP, YFP, and eNOS(1-33)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 2, 2014

Availability

Academic Institutions and Nonprofits only
pUAS-MmTFP13xmyc

Plasmid

#86371

Purpose

membrane targeted Tfp1 expression in zebrafish

Depositor

Rachel Wong

Article

Yoshimatsu et al Nat Commun. 2016 Feb 3;7:10590. doi: 10.

Insert

mTfp1

Use

Zebrafish

Tags

3xMyc and GAP43 membrane targeting

Expression

Mutation

Promoter

UAS

Available since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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Destination Vector

Plasmid

#125754

Purpose

Destination vector for assembling gRNA fragments in the second round of assembly.

Depositor

Rodrigo Ledesma-Amaro

Article

McCarty et al ACS Synth Biol. 2019 Apr 2. doi: 10.1021

Insert

pTDH3-GFPdropout-tTDH1

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

July 11, 2019

Availability

Academic Institutions and Nonprofits only

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pH2B-mPlum/Gag

Plasmid

#102255

Purpose

co-expression of Gag and H2B Plum in mammalian cells

Depositor

Suliana Manley

Article

Gunzenhauser et al PLoS One. 2014 Dec 10;9(12):e115095. doi

Inserts

Gag
H2B Plum

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA-HIRAmut-polyA

Plasmid

#59782

Purpose

This Hira gene contains silent mutations in siRNA-targeted sequences (see Inoue and Zhang, 2014 NSMB).

Depositor

Yi Zhang

Article

Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

Insert

HIRA

Use

Tags

Expression

Mammalian

Mutation

Change from 'GATCGAAGTTTG' to 'GAc…

Promoter

CMV and T7

Available since

Sept. 16, 2014

Availability

Academic Institutions and Nonprofits only

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DPY-pNCS

Plasmid

#17365

Depositor

Stephen Goff

Article

Yuan et al J Virol. 2000 Aug . 74(16):7250-60.

Insert

MMLV proviral DNA

Use

Tags

Expression

Mutation

deletion that removes 5 amino acids (DPPPY) from …

Promoter

Available since

March 1, 2010

Availability

Academic Institutions and Nonprofits only
BASIC_10_J23101-RBS32-GFP-B0015

Plasmid

#68144

Purpose

storage vector for BASIC part: GFP expression cassette

Depositor

Geoffrey Baldwin

Article

Storch et al ACS Synth Biol. 2015 Apr 16.

Insert

J23101-RBS32-GFP-B0015

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 19, 2016

Availability

Academic Institutions and Nonprofits only

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BASIC_12_ATG-GFP-B0015

Plasmid

#68146

Purpose

storage vector for BASIC part: GFP(ORF)

Depositor

Geoffrey Baldwin

Article

Storch et al ACS Synth Biol. 2015 Apr 16.

Insert

ATG-GFP-B0015

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 21, 2015

Availability

Academic Institutions and Nonprofits only

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XE169 mouse Wnt-1-myc-pGEM4

Plasmid

#16855

Depositor

Randall Moon

Article

McMahon et al Cell. 1989 Sep 22;58(6):1075-84. doi: 10

Insert

Wnt-1-myc (Wnt1 Mouse)

Use

Xenopus expression

Tags

Myc

Expression

Mutation

Promoter

Available since

Sept. 13, 2012

Availability

Academic Institutions and Nonprofits only
pET26b(modified)-T-Lg

Plasmid

#216977

Purpose

Expression of GvpT-Linker-Large BiT

Depositor

George Lu

Article

Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

Insert

GvpT-Linker-Large BiT

Use

Tags

Split Nanoluciferase LargeBiT-FLAG

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pST39-Mega'-deltaBSU

Plasmid

#216953

Purpose

Mega' with knockout of GvpB, GvpS and GvpU

Depositor

George Lu

Article

Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

Insert

Mega' with knockout of GvpB, GvpS and GvpU

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pST39-Mega'-deltaBRG

Plasmid

#216945

Purpose

Mega' with knockout of GvpB, GvpR and GvpG

Depositor

George Lu

Article

Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

Insert

Mega' with knockout of GvpB, GvpR and GvpG

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pET26b(modified)-Sm-U

Plasmid

#217003

Purpose

Expression of Small BiT-Linker-GvpU

Depositor

George Lu

Article

Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

Insert

Small BiT-Linker-GvpU

Use

Tags

Split Nanoluciferase SmallBiT

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 8, 2024

Availability

Academic Institutions and Nonprofits only

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