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pALPS rtTA3_V14

Plasmid

#101350

Purpose

Codon optimized rtTA3 used in trans with Tet inducible HIV-1

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

rtTA3

Use

Lentiviral

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pKM410

Plasmid

#134212

Purpose

Baculovirus expression of CENP-T and CENP-W

Depositor

Iain Cheeseman

Article

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

GST-CENP-T+W

Expression

Insect

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pSL1045

Plasmid

#129525

Purpose

pDEF-SpCas9, Empty Vector for CRISPRi, PbEF1a promoter, 1st Generation

Depositor

Scott Lindner

Article

Walker et al J Biol Chem. 2019 Jun 14;294(24):9555-95

Insert

dSpCas9 (Other)

Use

Plasmodium, rodent-infectious species

Available Since

Sept. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pKM463

Plasmid

#134214

Purpose

Baculovirus expression of CENP-U and CENP-Q

Depositor

Iain Cheeseman

Article

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

his-CENP-U + Q

Expression

Insect

Available Since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pAL-pyk_T

Plasmid

#74070

Purpose

pAL374 plasmid carrying the pyk (T) sgRNA targeting the template strand of pyk, SpecR

Depositor

Timothy Lu

Article

Cleto et al ACS Synth Biol. 2016 Feb 16.

Insert

sgRNA pyk (T)

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

ptac

Available Since

April 12, 2016

Availability

Academic Institutions and Nonprofits only
p4580 AU-IRF-3a

Plasmid

#8660

Depositor

Peter Howley

Article

Karpova et al Mol Cell Biol 2001 Jul;21(13):4169-76.

Insert

IRF-3a (IRF3 Human)

Tags

AU

Expression

Mammalian

Available Since

June 16, 2005

Availability

Academic Institutions and Nonprofits only
M16 pCS-twhh-bGFP

Plasmid

#17153

Depositor

Randall Moon

Article

Du et al J Cell Biol. 1997 Oct 6. 139(1):145-56.

Insert

twhh promoter (shhb Zebrafish)

Tags

bGFP

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pJMP2367

Plasmid

#160076

Purpose

Z. mobilis CRISPRi, promoter C, empty guide, sfGFP

Depositor

Jason Peters

Article

Banta et al Appl Environ Microbiol. 2020 Nov 10;86(2

Insert

empty sgRNA

Expression

Bacterial

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pKM610

Plasmid

#134218

Purpose

Baculovirus expression of CENP-R

Depositor

Iain Cheeseman

Article

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

CENP-R untagged

Expression

Insect

Available Since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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jk089_h12

Plasmid

#112151

Purpose

C. thermophilum DASH/Dam1 complex (construct used for cryo-EM structure determination)

Depositor

Stephen Harrison

Article

Jenni et al Science. 2018 May 4;360(6388):552-558. d

Insert

C. thermophilum DASH/Dam1 complex

Tags

his tag on Spc34 and strep tag on Dad1

Available Since

Sept. 20, 2018

Availability

Academic Institutions and Nonprofits only

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p413TEF-HST2

Plasmid

#51745

Purpose

overexpression of HST2 in S. cerevisiae, HIS3 marker

Depositor

Markus Ralser

Article

Ralser et al PLoS One. 2009;4(4):e5376. doi: 10.1371/

Insert

HST2 (HST2 Budding Yeast)

Expression

Yeast

Promoter

TEF1

Available Since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

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pKM568

Plasmid

#134217

Purpose

Baculovirus expression of CENP-C

Depositor

Iain Cheeseman

Article

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

GST-CENP-C

Expression

Insect

Available Since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pJMP2480

Plasmid

#160080

Purpose

Z. mobilis CRISPRi, promoter C, empty guide

Depositor

Jason Peters

Article

Banta et al Appl Environ Microbiol. 2020 Nov 10;86(2

Insert

empty sgRNA

Expression

Bacterial

Available Since

Nov. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pXCX.Syn.tTA

Plasmid

#38058

Depositor

James Uney

Article

Lee et al J Gene Med. 2005 May;7(5):576-83.

Insert

tTA

Use

Adenoviral

Expression

Mammalian

Promoter

Synapsin (human SYN1)

Available Since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only
pWZL Hygro- TRF2 deltaM

Plasmid

#18012

Depositor

Titia de Lange

Article

Karlseder et al Science. 2002 Mar 29. 295(5564):2446-9.

Insert

hTRF2 (TERF2 Human)

Use

Retroviral

Expression

Mammalian

Mutation

Deleted Myb Domain (aa454-500)

Available Since

May 29, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA3-myc-p110α(K227E)

Plasmid

#39491

Depositor

Julian Downward

Article

Rodriguez-Viciana et al EMBO J. 1996 May 15;15(10):2442-51.

Insert

p110(K227E) (PIK3CA Bovine)

Tags

myc

Expression

Mammalian

Mutation

K227E

Promoter

CMV

Available Since

Oct. 26, 2012

Availability

Academic Institutions and Nonprofits only
pWZL Hygro-TRF2 deltaB

Plasmid

#18010

Depositor

Titia de Lange

Article

Karlseder et al Science. 2002 Mar 29. 295(5564):2446-9.

Insert

hTRF2 (TERF2 Human)

Use

Retroviral

Expression

Mammalian

Mutation

Deleted Basic Domain (aa1-44)

Available Since

May 29, 2008

Availability

Academic Institutions and Nonprofits only
pTRE3G-Fluc-DR

Plasmid

#242434

Purpose

Firefly Luciferse with a Direct Repeat sequence in the 3’UTR recognised by the CasRx under the control of the TRE3G promoter.

Depositor

Diego Di Bernardo

Article

De Carluccio et al Nat Commun. 2024 Apr 17;15(1):3311. doi:

Insert

Firefly Luciferase

Use

CRISPR and Luciferase

Tags

CasRx Direct Repeat

Available Since

Nov. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pDL1-Spol-foxA-C-term

Plasmid

#242944

Purpose

Encodes a C-terminal fragment of Spol-foxA to generate dsRNA for RNAi experiments in the planarian S. polychroa

Depositor

Erin Davies

Article

Deihl et al bioRxiv [Preprint]. 2025 Jul 22:2025.07.

Insert

Spol-foxA

Use

RNAi

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSpy

Plasmid

#220184

Purpose

sgRNA expression vector - promoterless backbone

Depositor

Larry Halverson

Article

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

promoterless backbone

Use

CRISPR

Expression

Bacterial

Promoter

promoterless

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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