We narrowed to 2,317 results for: pET28

1. pET28a-matNODALvar-HIS

   Plasmid

   #115265

   Purpose

   For bacterial recombinant protein production of the mature peptide of a human NODAL splice variant

   Depositor

   Lynne Postovit

   Article

   Findlay et al (2018) bioRxiv 276170

   Insert

   NODAL variant mature peptide (NODAL Human)
   

   Use

   Tags

   6His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a-eGFP-Pep-H

   Plasmid

   #185788

   Purpose

   Encodes enhanced green flourescent protein linked to Pep-H self-assembling peptide via a GS-linker sequence

   Depositor

   Lizhong He

   Article

   Shanbhag et al Adv. Funct. Mater. 2022, 32, 2108039

   Insert

   eGFP-Pep4
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28b-MBP-bhaB5

   Plasmid

   #167812

   Purpose

   Expresses His6- and MBP-tagged BhaB5 in E. coli

   Depositor

   Wilfred van der Donk

   Article

   Daniels et al Nat Chem. 2022 Jan;14(1):71-77. doi: 10.

   Insert

   BhaB5
   

   Use

   Tags

   His6, MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET28_redesigned_Caspase-4_ANR261ENP_D315H_M318K_T352K_enzymatic domain

   Plasmid

   #183387

   Purpose

   Overexpression of catalytic domain of inflammatory caspase in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

   Insert

   redesigned Caspase-4 (AA94-377) (CASP4 Human)
   

   Use

   Tags

   His-TEV

   Expression

   Bacterial

   Mutation

   ANR261ENP, D315H, M318K, T352K

   Promoter

   T7

   Available since

   July 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a Cdk2ap1ΔN (MT2B2)

   Plasmid

   #178034

   Purpose

   Expression vector for the purpose of protein purification.

   Depositor

   Lin He

   Article

   Modzelewski et al Cell. 2021 Oct 7. pii: S0092-8674(21)011

   Insert

   Cdk2ap1 (Cdk2ap1 Mouse)
   

   Use

   Nonviral

   Tags

   6xHIS - Thrombin - MBP- TEV-TRS

   Expression

   Bacterial

   Mutation

   First N-Terminal 27aa are deleted

   Promoter

   T7LacI

   Available since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. 6xHis-CPL2/pET28a

   Plasmid

   #172095

   Purpose

   cDNA of CER11/CPL2 cloned into pET28a for protein expression

   Depositor

   George Haughn

   Article

   Shi et al Plant Physiol. 2019 Nov;181(3):901-915.

   Insert

   CPL2 (CPL2 Mustard Weed)
   

   Use

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 20, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pET28_6xHis-PARP1delZn1-L713F

   Plasmid

   #173937

   Purpose

   Bacterial expression of PARP1 lacking Zn1 domain but containing L713F gain-of-function mutation

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1delZn1-L713F (PARP1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Deletion of residues 1-96; L713F, V762A

   Promoter

   Available since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28_6xHis-PARP1delZn3-L713F

   Plasmid

   #173934

   Purpose

   Bacterial expression of PARP1 lacking Zn3 domain but containing L713F gain-of-function mutation

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1delZn3-L713F (PARP1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Deletion of residues 214-373; L713F, V762A

   Promoter

   Available since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28_6xHis-PARP1-G871L

   Plasmid

   #174798

   Purpose

   Bacterial expression of a hyperactive PARP1 mutant (G871L destabilizes the autoinhibitory HD subdomain)

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-G871L (PARP1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   G871L, V762A

   Promoter

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28_6xHis-PARP1-A870L

    Plasmid

    #174797

    Purpose

    Bacterial expression of a hyperactive PARP1 mutant (A870L destabilizes the autoinhibitory HD subdomain)

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-A870L (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    A870L, V762A

    Promoter

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET28_6xHis-PARP1-K893I

    Plasmid

    #174799

    Purpose

    Bacterial expression of an inactive mutant (K893I may disrupt NAD+ binding)

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-K893I (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    K893I, V762A

    Promoter

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET28a-XPO5-His6

    Plasmid

    #171562

    Purpose

    Encodes human XPO5 protein

    Depositor

    Yan Zeng

    Insert

    XPO5 (XPO5 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET28_hTLN2-head-LBD

    Plasmid

    #164837

    Purpose

    Expression of human talin2 head mutant

    Depositor

    Tina Izard

    Article

    Rangarajan et al J Biol Chem. 2020 Sep 11;295(37):12885-1

    Insert

    TLN2 (TLN2 Human)
    

    Use

    Tags

    His8

    Expression

    Bacterial

    Mutation

    Lipid binding deficient mutations

    Promoter

    Available since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET28b-GFP-tglG

    Plasmid

    #134679

    Purpose

    Expresses His6-GFP-TglG fusion in E. Coli

    Depositor

    Wilfred van der Donk

    Article

    Ting et al Science. 2019 Jul 19;365(6450):280-284.

    Insert

    TglG
    

    Use

    Tags

    His6-GFP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET28a SznF E281A

    Plasmid

    #139591

    Purpose

    expresses SznF E281A

    Depositor

    Emily Balskus

    Article

    Ng et al Nature. 2019 Feb;566(7742):94-99. doi: 1

    Insert

    SznF E281A
    

    Use

    Tags

    hexahistidine

    Expression

    Bacterial

    Mutation

    changed glutamate 281 to alanine

    Promoter

    T7

    Available since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET28a SznF H225A

    Plasmid

    #139590

    Purpose

    expresses SznF H225A

    Depositor

    Emily Balskus

    Article

    Ng et al Nature. 2019 Feb;566(7742):94-99. doi: 1

    Insert

    SznF H225A
    

    Use

    Tags

    hexahistidine

    Expression

    Bacterial

    Mutation

    changed histidine 225 to alanine

    Promoter

    T7

    Available since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28a-SznF E215A

    Plasmid

    #139565

    Purpose

    expresses SznF E215A in E. coli

    Depositor

    Emily Balskus

    Article

    Ng et al Nature. 2019 Feb;566(7742):94-99. doi: 1

    Insert

    SznF E215A
    

    Use

    Tags

    hexahistidine

    Expression

    Bacterial

    Mutation

    changed glutamate 215 to alanine

    Promoter

    T7

    Available since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET28a-DDX43-NT-G46D

    Plasmid

    #134588

    Purpose

    Express human DDX43 N-terminal region (1-253 aa, with G46D mutation) in E. coli

    Depositor

    Yuliang Wu

    Article

    Talwar et al J Biol Chem. 2017 Jun 23;292(25):10429-1

    Insert

    DDX43-N-terminal (1-253 aa) with G46D mutation (DDX43 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    full-length (G154D)

    Promoter

    T7

    Available since

    Jan. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28a-DDX43-FL-G84D

    Plasmid

    #134577

    Purpose

    Express human DDX43 mutant (full-length with G84D mutation) in E. coli

    Depositor

    Yuliang Wu

    Article

    Talwar et al J Biol Chem. 2017 Jun 23;292(25):10429-1

    Insert

    DDX43-G84D (DDX43 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    full-length (G84D)

    Promoter

    T7

    Available since

    Jan. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28B-PU.1-ETS-Wing

    Plasmid

    #124552

    Purpose

    Chimeric ETS domain: Wing of Ets-1 in PU.1 scaffold

    Depositor

    Gregory Poon

    Article

    Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

    Insert

    PU.1 ETS domain (residues 167-272) (Ets1, Spi1 Mouse)
    

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    The 5 residues making up the "wing" in …

    Promoter

    Available since

    June 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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