We narrowed to 5,839 results for: pcas

1. pCAGGS-Trex2

   Plasmid

   #79246

   Purpose

   Expression vector for Trex2, which is a non-processive 3' exonuclease.

   Depositor

   Jeremy Stark

   Article

   Bennardo et al PLoS Genet. 2009 Oct;5(10):e1000683. doi

   Insert

   Trex2 (Trex2 Mouse)
   

   Expression

   Mammalian

   Promoter

   pCAGGS

   Available Since

   Aug. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAGGS::AtHAP2-V5

   Plasmid

   #132958

   Purpose

   Express AtHAP2 full length protein in mammalian cells

   Depositor

   Benjamin Podbilewicz

   Article

   Valansi et al J Cell Biol. 2017 Mar 6;216(3):571-581.

   Insert

   AtHAP2 (GCS1, HAP2 Mustard Weed)
   

   Tags

   V5

   Expression

   Mammalian

   Available Since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-mCherry2-G

   Plasmid

   #164162

   Purpose

   Expresses mCherry2 under the control of CAG promoter, basic vector, multiclonig site at C terminal

   Depositor

   Isei Tanida

   Article

   Tanida et al Sci Rep. 2020 Dec 14;10(1):21871. doi: 1

   Insert

   mCherry2
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Feb. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAT.015

   Plasmid

   #119558

   Purpose

   Level T acceptor vector for chromosomal integration with lacZ compatible with blue/white screening. Modified pUC19 vector.

   Depositor

   Alistair McCormick

   Article

   Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

   Type

   Empty backbone

   Use

   Synthetic Biology

   Available Since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG.Myc-SARS2_3CL

   Plasmid

   #200120

   Purpose

   Expresses N-terminal Myc-tagged human codon optimyzed SARS-CoV-2 3CL in mammalian cells

   Depositor

   Eiji Morita

   Article

   Arakawa et al Sci Rep. 2023 Jan 31;13(1):1753. doi: 10

   Insert

   SARS-CoV-2 3CL protease (ORF1ab SARS-CoV-2)
   

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEF1a-nSpCas9-NG

   Plasmid

   #171692

   Purpose

   Expresses nSpCas9-NG in mammalian cells

   Depositor

   Jia Chen

   Article

   Wang et al Nat Cell Biol. 2021 May 10. pii: 10.1038

   Insert

   nSpCas9-NG
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1…

   Promoter

   EF1a

   Available Since

   Aug. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. KA2601_eSpCas9-G2P

   Plasmid

   #124203

   Purpose

   Expression of eSpCas9(1.1) and sgRNA

   Depositor

   Hans Schöler

   Article

   Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   March 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. AV44_pCAG.Cas9-D10A.gRNA.S1

   Plasmid

   #199258

   Purpose

   Expression construct encoding SpCas9-D10A nickase and an AAVS1-targeting guide RNA

   Depositor

   Manuel Goncalves

   Article

   Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

   Inserts

   SpCas9-D10A nickase
   AAVS1-targeting gRNA
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   CAG promoter and RNA polymerase III promoter for …

   Available Since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX330-HA-dSpCas9

   Plasmid

   #92112

   Purpose

   Expression plasmid for human codon-optimized dead/inactive SpCas9 (without U6-sgRNA coding sequence)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Insert

   dead/inactive SpCas9
   

   Use

   CRISPR

   Tags

   HA tag and NLS

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   Cbh

   Available Since

   Oct. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAFNF-tdTomato

    Plasmid

    #125575

    Purpose

    Flp-dependent expression of tdTomato in mammalian cells.

    Depositor

    Takeshi Imai

    Article

    Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

    Insert

    tdTomato
    

    Expression

    Mammalian

    Available Since

    July 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-Gdf9

    Plasmid

    #163599

    Purpose

    Expresses GDF-9 in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Gdf9 (Gdf9 Mouse)
    

    Expression

    Mammalian

    Available Since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-Rad18

    Plasmid

    #119003

    Purpose

    Mammalian expression of human Rad18 and BFP

    Depositor

    Ralf Kuehn

    Article

    Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

    Insert

    Rad18
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCaSpeR-act5cB-QF#7

    Plasmid

    #46125

    Depositor

    Christopher Potter

    Article

    Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

    Insert

    QF#7
    

    Expression

    Insect

    Available Since

    Aug. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-T3-st1CAS9-pA

    Plasmid

    #131542

    Purpose

    Expresses S. thermophilus 1-derived Cas9 nuclease in mammalian cells. Used to modify genome of mouse embroys

    Depositor

    Wataru Fujii

    Article

    Fujii et al Biochem Biophys Res Commun. 2016 Jun 16.

    Insert

    Codon optimized st1Cas9
    

    Use

    CRISPR

    Tags

    Flag, NLS, and polyA

    Expression

    Mammalian

    Available Since

    Nov. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAR-IF reporter

    Plasmid

    #16628

    Depositor

    Bert Vogelstein

    Article

    Nicolaides et al Mol Cell Biol. 1998 Mar . 18(3):1635-41.

    Insert

    CAR-IF
    

    Tags

    beta-galactosidase

    Expression

    Mammalian

    Available Since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

16. pCAG-V5-NLGN2

    Plasmid

    #82538

    Purpose

    pCAG vector for expressing V5-NLGN2

    Depositor

    Alice Ting

    Article

    Loh et al Cell. 2016 Aug 25;166(5):1295-1307.e21.

    Insert

    NLGN2 (Nlgn2 Rat)
    

    Tags

    V5

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

17. pCAG-mWasabi-ER

    Plasmid

    #164165

    Purpose

    Expresses mWasabi fused with an ER-targeting sequence of calreticulin and the ER retrieval sequence, KDEL, under the control of CAG promoter

    Depositor

    Isei Tanida

    Article

    Tanida et al Sci Rep. 2020 Dec 14;10(1):21871. doi: 1

    Insert

    mWasabi
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Feb. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. SpCas9 TadCBEb

    Plasmid

    #193832

    Purpose

    Express TadCBEb (with SpCas9) in mammalian cells

    Depositor

    David Liu

    Article

    Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

    Insert

    TadA-CDb-SpCas9 D10A-2xUGI
    

    Expression

    Mammalian

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCas9CR4-VQR

    Plasmid

    #120224

    Purpose

    pCas9CR4 with VQR mutations for changing PAM site specificity to NGAN or NGNG from Kleinstiver et al. 2015

    Depositor

    Christopher Reisch

    Insert

    SpCas9-VQR
    

    Use

    Synthetic Biology

    Tags

    ssrA

    Mutation

    Mutations D1135V/R1335V/T1337R

    Promoter

    pTet

    Available Since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCasper4-QF#7-hsp70

    Plasmid

    #46135

    Depositor

    Christopher Potter

    Article

    Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

    Insert

    QF#7
    

    Expression

    Insect

    Available Since

    Aug. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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