We narrowed to 14,076 results for: Mpl;

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pcDNA5-FRT-TO-Nsp15-HF

Plasmid

#157722

Purpose

mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp15 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp15-HF (ORF1ab SARS-CoV-2)

Tags

His8-Flag

Expression

Mammalian

Mutation

codon optimized

Available Since

Aug. 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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HOXB3 (human) HIS-tag pET

Plasmid

#8524

Depositor

Corey Largman

Article

Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

Insert

HOXB3 (HOXB3 Human)

Tags

His and T7

Expression

Bacterial

Mutation

Contains 5 additional amino acids before ATG from…

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pJZC73

Plasmid

#62341

Purpose

sgRNA (no RNA aptamer addition) with COM-KRAB effector for mammalian cells

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Inserts

sgRNA
COM-KRAB

Use

Lentiviral

Tags

KRAB

Expression

Mammalian

Mutation

Targets sv40 promoter, sequence: GAATAGCTCAGAGGCC…

Promoter

CMV and U6

Available Since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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pBABE hygro MEN1 T344R

Plasmid

#11019

Depositor

Matthew Meyerson

Article

Hughes et al Mol Cell. 2004 Feb 27. 13(4):587-97.

Insert

MEN1 T344R (MEN1 Human)

Use

Retroviral

Expression

Mammalian

Mutation

T344R

Available Since

Dec. 2, 2005

Availability

Academic Institutions and Nonprofits only
EZH2-TALEN_Right_No_FLAG

Plasmid

#79900

Purpose

Untagged EZH2-TALEN_Right to target the N-terminus of human EZH2

Depositor

Yannick Doyon

Article

Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

Insert

EZH2-TALEN_Right_No_FLAG (EZH2 Human)

Use

TALEN

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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[pSK497] pLJC5 Flag-Depdc5(AB)

Plasmid

#109341

Purpose

Lenti-viral expression of Flag-tagged Depdc5 mutant AB

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Nature. 2018 Apr 5;556(7699):64-69. doi:

Insert

Depdc5 (DEPDC5 Human)

Use

Lentiviral

Tags

FLAG

Mutation

DIYGD(185-189)GSGSG;EQPLH(371-375)GSGSG

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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HA-VHL 1-187-pRc/CMV

Plasmid

#22079

Depositor

William Kaelin

Article

Lonergan et al Mol Cell Biol. 1998 Feb . 18(2):732-41.

Insert

von Hippel-Lindau tumor suppressor (VHL) (VHL Human)

Tags

HA-tag

Expression

Mammalian

Mutation

deletion mutant covering only aa 1-187 of wt VHL …

Available Since

Sept. 14, 2009

Availability

Academic Institutions and Nonprofits only

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pBABE hygro MEN1 W436R

Plasmid

#11018

Depositor

Matthew Meyerson

Article

Hughes et al Mol Cell. 2004 Feb 27. 13(4):587-97.

Insert

MEN1 W436R (MEN1 Human)

Use

Retroviral

Expression

Mammalian

Mutation

W436R

Available Since

Dec. 2, 2005

Availability

Academic Institutions and Nonprofits only
pU6-(BsaI)_CBh-Cas9

Plasmid

#135010

Purpose

Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9

Depositor

Yosef Shaul

Article

Reuven et al Biomolecules. 2019 Oct 8;9(10). pii: bio

Insert

humanized S. pyogenes Cas9

Tags

3xFLAG (N terminal on insert)

Expression

Mammalian

Promoter

CBh

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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Gal4-entry

Plasmid

#128014

Purpose

Vector containing the Gal4-entry expression cassette and a separate expression cassette driving mCherry via the traffic jam enhancer

Depositor

Julius Brennecke

Article

Batki et al Nat Struct Mol Biol. 2019 Aug;26(8):720-

Insert

Gal4-DBD-3xFlag

Use

Gal4 entry vector

Tags

Gal4-DBD-3xFlag

Available Since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.3 HA-TSPAN12^11-LEL

Plasmid

#115785

Purpose

Expresses a chimera in mammalian cells: the large extracellular loop of TSPAN12 is replaced with TSPAN11 sequence

Depositor

Harald Junge

Article

Lai et al Cell Rep. 2017 Jun 27;19(13):2809-2822.

Insert

TSPAN12/11 chimera (TSPAN11, TSPAN12 Human)

Tags

HA

Expression

Mammalian

Mutation

large extracellular loop of TSPAN12 replaced with…

Promoter

CMV

Available Since

Oct. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pET14b-mEKLF

Plasmid

#67834

Purpose

Encodes murine EKLF (nucleotides 71-1215) for in vitro chromatin/transcription reconstitution

Depositor

James Bieker

Article

Armstrong et al Cell. 1998 Oct 2;95(1):93-104.

Insert

EKLF (Klf1 Mouse)

Expression

Bacterial

Mutation

Full-length mEKLF is aa 20-376; amino acid 19 is …

Promoter

T7

Available Since

Aug. 25, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA5FRT/TO PP1gamma-2*Strep

Plasmid

#113506

Purpose

Expresses dual Strep-tagged PP1gamma in mammalian cells

Depositor

Hemmo Meyer

Article

Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

Insert

PP1gamma (PPP1CC Human)

Tags

Strep-Strep

Expression

Mammalian

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pBABE hygro MEN1 P12L

Plasmid

#11020

Depositor

Matthew Meyerson

Article

Hughes et al Mol Cell. 2004 Feb 27. 13(4):587-97.

Insert

MEN1 P12L (MEN1 Human)

Use

Retroviral

Expression

Mammalian

Mutation

P12L

Available Since

Dec. 2, 2005

Availability

Academic Institutions and Nonprofits only

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pBABE hygro MEN1 A309P

Plasmid

#11023

Depositor

Matthew Meyerson

Article

Hughes et al Mol Cell. 2004 Feb 27. 13(4):587-97.

Insert

MEN1 A309P (MEN1 Human)

Use

Retroviral

Expression

Mammalian

Mutation

A309P

Available Since

Dec. 2, 2005

Availability

Academic Institutions and Nonprofits only

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pCMV10/3xflag-Clock L57E/L113E/W284A

Plasmid

#47343

Purpose

triple mutation used for co-IP

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

Clock (Clock Mouse)

Tags

3x Flag

Expression

Mammalian

Mutation

L57E/L113E/W284A

Promoter

CMV

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
pBScMXT + mSlo1 MBr5/23

Plasmid

#16151

Depositor

Larry Salkoff

Article

Butler et al Science. 1993 Jul 9. 261(5118):221-4.

Insert

Mus musculus potassium large conductance calcium-activated channel (Kcnma1 Mouse)

Use

Xenopus oocyte expression

Mutation

N/A

Available Since

Jan. 10, 2008

Availability

Academic Institutions and Nonprofits only
p28BIOHTEV-LIC: nsp16_SARS-CoV-2

Plasmid

#167846

Purpose

Bacterial expression of the SARS-CoV-2 protein nsp16

Depositor

Cheryl Arrowsmith

Article

Perveen et al SLAS Discov. 2021 Jan 10:247255522098504

Insert

nsp16

Tags

6xHis and AviTag

Expression

Bacterial

Promoter

T7

Available Since

April 16, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pmiRGLO-LFNG-203

Plasmid

#117351

Purpose

Dual luciferase assay for miRNA-targeted UTR

Depositor

Volker Busskamp

Article

Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

Insert

3' UTR LFNG-203

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Promoter

Murine PGK-1

Available Since

Dec. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA3-HA/Bmal I317D

Plasmid

#47347

Purpose

PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

BMAL (Bmal1 Mouse)

Tags

Ha

Expression

Mammalian

Mutation

I317D

Promoter

CMV

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only