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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 18,351 results for: multi

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Showing: 7381 - 7400 of 18351 results

  2. pTPH413

    Plasmid
    #185728
    Purpose
    Bacterial PAM profiling of adenine base editor variants
    Depositor
    David Liu
    Publication
    Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038
    Inserts
    ecTadA(8e)-neNme2-C
    Nme2Cas9 sgRNA
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    Nme2Cas9 P6S/D16A/E33G/K104T/D152A/F260L/A263T/A3…
    Available since
    Sept. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    185728-plasmid-map-sequence-id-364088

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  4. pDAS12342_PEAR-GFP_2in1

    Plasmid
    #177184
    Purpose
    PEAR-GFP plasmid with a pegRNA that targets its own plasmid
    Depositor
    Ervin Welker
    Publication
    Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    EGFP split with an intron between amino acids 95-96
    pegRNA targeting its own plasmid
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    disrupted 5' splice site
    Promoter
    CMV and U6
    Available since
    Feb. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    177184-plasmid-map-sequence-id-348471

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  6. SGyA

    Plasmid
    #160292
    Purpose
    Drives expression of Cas9 and a TdTomato fluorescent marker
    Depositor
    Omar Akbari
    Publication
    Gamez et al Nat Commun. 2021 Dec 10;12(1):7202. doi:
    Inserts
    Vas promoter
    hSpCas9
    tdTomato
    CTCF insulator
    Gypsy insulator
    Left homology arm Y chromosome
    Right homology arm Y chromosome
    Tags
    T2A GFP
    Expression
    Insect
    Available since
    March 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    160292-plasmid-map-sequence-id-316542

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  7. pUltra-hot-H2B-mTagBFP2

    Plasmid
    #161792
    Purpose
    3rd generation lentiviral multicistronic vector for mammalian expression of mCherry, H2B-mTagBFP2, and one gene of interest
    Depositor
    Jorge Luis Arias-Arias
    Insert
    H2B-mTagBFP2
    Use
    Lentiviral; Multicistronic
    Tags
    mCherry and mTagBFP2
    Expression
    Mammalian
    Mutation
    I174A in the mTagBFP2 protein according to PLoS O…
    Promoter
    UbC
    Available since
    Nov. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    161792-plasmid-map-sequence-id-316815

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  9. pAAV CAG ChR2 E123T T159C 2A tDimer

    Plasmid
    #85399
    Purpose
    High efficiency channelrhodopsin with cytomegalovirus enhancer fused to chicken β-actin (CAG) promoter
    Depositor
    Thomas Oertner
    Publication
    Berndt et al Proc Natl Acad Sci U S A. 2011 May 3;108
    Inserts
    Channelrhodopsin-2
    red fluorescent protein
    Use
    AAV
    Expression
    Mammalian
    Mutation
    E123T , T159C
    Promoter
    CAG and ribosomal skip sequence 2A
    Available since
    Jan. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    85399-plasmid-map-sequence-id-201309

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  11. pDule-IBBN (G2)

    Plasmid
    #85500
    Purpose
    Plasmid for incorporating the non-canonical amino acid IBBN with the Mj IBBN (G2) synthetase and cognate amber suppressing tRNA in Ecoli.
    Depositor
    Ryan Mehl
    Publication
    Peeler et al J Am Chem Soc. 2010 Oct 6;132(39):13575-
    Insert
    IBBN (G2) synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    Expression
    Bacterial
    Mutation
    Y32G L65E F108W Q109M D158S R162K
    Promoter
    lpp (constitutive)
    Available since
    March 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    85500-plasmid-map-sequence-id-169863

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  13. pH2B-SCFP1-2A-mTq2-NES

    Plasmid
    #60491
    Purpose
    Co-expresses two cyan fluorescent protein variants, a short fluorescence lifetime CFP variant in the nucleus and a CFP variant with a long lifetime (mTurquoise2) in the cytoplasm
    Depositor
    Dorus Gadella
    Publication
    Joosen et al J Microsc. 2014 Sep 1. doi: 10.1111/jmi.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    H2B-SCFP1
    mTurquoise2-NES
    Tags
    SCFP1 and mTurquoise2
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 31, 2014
    Availability
    Academic Institutions and Nonprofits only
    60491-plasmid-map-sequence-id-96973

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  14. Cenp-B INCENP mCherry

    Plasmid
    #45235
    Purpose
    Target INCENP to centromeres via full length CENP-B
    Depositor
    Michael Lampson
    Publication
    Wang et al J Cell Biol. 2011 Aug 22;194(4):539-49.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cenp-B, INCENP-Δcen, mCherry
    Tags
    full length CENP-B and mCherry
    Expression
    Mammalian
    Mutation
    CEN box deleted from INCENP (aa1-46)
    Available since
    Sept. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    45235-plasmid-map-sequence-id-84488

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  18. rTetR-GFP-VP16

    Plasmid
    #183917
    Purpose
    VP16 activation domain coupled to reverse Tet repressor; labeled with GFP; binds tetO sites upon doxycycline addition
    Depositor
    Karsten Rippe
    Publication
    Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    rTetR-GFP-VP16 (UL48 Synthetic)
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    June 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    183917-plasmid-map-sequence-id-364155

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  19. pVenusCfC

    Plasmid
    #128567
    Purpose
    Protein interaction screening tool with Gateway-compatible BiFC vectors comprising of a set of 4 vectors permiting N or C fusions of candidate genes to the N or C fragment of Split-Venus.
    Depositor
    Oliver Vugrek
    Publication
    Lepur et al J Biomol Screen. 2016 Dec;21(10):1100-11
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Venus
    Expression
    Mammalian
    Available since
    Jan. 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    128567-plasmid-map-sequence-id-321691

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  20. pVenusCfN

    Plasmid
    #128566
    Purpose
    Protein interaction screening tool with Gateway-compatible BiFC vectors comprising of a set of 4 vectors permiting N or C fusions of candidate genes to the N or C fragment of Split-Venus.
    Depositor
    Oliver Vugrek
    Publication
    Lepur et al J Biomol Screen. 2016 Dec;21(10):1100-11
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Venus
    Expression
    Mammalian
    Available since
    Jan. 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    128566-plasmid-map-sequence-id-513794

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Showing: 7381 - 7400 of 18351 results