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Puro-Cas9 donor

Plasmid

#58409

Purpose

Donor vector for genomic targeting of a Tetracycline-inducible Cas9 cassette to the human AAVS1/PPP1R12C locus

Depositor

Danwei Huangfu

Article

Gonzalez et al Cell Stem Cell. 2014 Jun 11. pii: S1934-

Insert

hSpCas9

Use

CRISPR

Tags

3xFlag, Nucleoplasmin NLS, and SV40 NLS

Expression

Mammalian

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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Puro-Cas9 donor

Plasmid

#58409

Purpose

Donor vector for genomic targeting of a Tetracycline-inducible Cas9 cassette to the human AAVS1/PPP1R12C locus

Depositor

Danwei Huangfu

Article

Gonzalez et al Cell Stem Cell. 2014 Jun 11. pii: S1934-

Insert

hSpCas9

Use

CRISPR

Tags

3xFlag, Nucleoplasmin NLS, and SV40 NLS

Expression

Mammalian

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pCASP-SptP120-3m160-HilA

Plasmid

#153325

Purpose

Triggers secretion of SptP120 fused to the 3m160 anti-mCherry DARPin from Salmonella upon induction with arabinose

Depositor

Josef Penninger

Article

Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

Insert

SptP120-3m160 DARPin

Use

Salmonella expression

Available Since

July 16, 2020

Availability

Academic Institutions and Nonprofits only

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pCASP-SptP120-3m160-HilA

Plasmid

#153325

Purpose

Triggers secretion of SptP120 fused to the 3m160 anti-mCherry DARPin from Salmonella upon induction with arabinose

Depositor

Josef Penninger

Article

Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

Insert

SptP120-3m160 DARPin

Use

Salmonella expression

Available Since

July 16, 2020

Availability

Academic Institutions and Nonprofits only

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ampl43

Plasmid

#161830

Purpose

pRS416-dCas9-Mxi1+TetR+pRPR1(TetO)-NotI-gRNA plasmid marked with His, expressing dCas9-Mxi1 under Tef1 promoter, and a tet-inducible promoter for gRNA expression.

Depositor

Saeed Tavazoie

Article

Momen-Roknabadi et al Commun Biol. 2020 Nov 27;3(1):723. doi:

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Promoter

TEF1

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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ampl43

Plasmid

#161830

Purpose

pRS416-dCas9-Mxi1+TetR+pRPR1(TetO)-NotI-gRNA plasmid marked with His, expressing dCas9-Mxi1 under Tef1 promoter, and a tet-inducible promoter for gRNA expression.

Depositor

Saeed Tavazoie

Article

Momen-Roknabadi et al Commun Biol. 2020 Nov 27;3(1):723. doi:

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Promoter

TEF1

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pJM220 (pUC18T-miniTn7T-gm-rhaSR-PrhaBAD)

Plasmid

#110559

Purpose

miniTn7 delivery plasmid with rhaSR-PrhaBAD inducible promoter

Depositor

Joanna Goldberg

Article

Meisner et al Appl Environ Microbiol. 2016 Oct 27;82(2

Insert

rhaSR-PrhaBAD

Expression

Bacterial

Promoter

rhaSR-PrhaBAD

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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pJM220 (pUC18T-miniTn7T-gm-rhaSR-PrhaBAD)

Plasmid

#110559

Purpose

miniTn7 delivery plasmid with rhaSR-PrhaBAD inducible promoter

Depositor

Joanna Goldberg

Article

Meisner et al Appl Environ Microbiol. 2016 Oct 27;82(2

Insert

rhaSR-PrhaBAD

Expression

Bacterial

Promoter

rhaSR-PrhaBAD

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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NLS-HA-tdMCP-tdTomato

Plasmid

#183938

Purpose

tandem MS2 binding protein labeled with tdTomato, containing a NLS and a HA-tag

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

tdMCP-tdTomato (cp Synthetic)

Tags

NLS-HA

Expression

Mammalian

Mutation

none

Promoter

CMV (TATA box removed)

Available Since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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NLS-HA-tdMCP-tdTomato

Plasmid

#183938

Purpose

tandem MS2 binding protein labeled with tdTomato, containing a NLS and a HA-tag

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

tdMCP-tdTomato (cp Synthetic)

Tags

NLS-HA

Expression

Mammalian

Mutation

none

Promoter

CMV (TATA box removed)

Available Since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1-TetON-Puro-HIF1a 3809

Plasmid

#118705

Purpose

Lentivirus for expression of shRNA1 against human HIF1a (Dox-inducible)

Depositor

William Kaelin

Article

Briggs et al Cell. 2016 Jun 30;166(1):126-39. doi: 10

Insert

shRNA against human HIF1a

Use

Lentiviral

Available Since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO.1-TetON-Puro-HIF1a 3809

Plasmid

#118705

Purpose

Lentivirus for expression of shRNA1 against human HIF1a (Dox-inducible)

Depositor

William Kaelin

Article

Briggs et al Cell. 2016 Jun 30;166(1):126-39. doi: 10

Insert

shRNA against human HIF1a

Use

Lentiviral

Available Since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pET3a-hUb

Plasmid

#61937

Purpose

Expression of hUb in E.coli

Depositor

Chris Hill

Article

VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

Insert

human ubiquitin (UBC Human)

Expression

Bacterial

Promoter

T7

Available Since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only
pET3a-hUb

Plasmid

#61937

Purpose

Expression of hUb in E.coli

Depositor

Chris Hill

Article

VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

Insert

human ubiquitin (UBC Human)

Expression

Bacterial

Promoter

T7

Available Since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only
FKBP-myc

Plasmid

#20211

Depositor

Carolyn Bertozzi

Article

Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

Insert

FKBP (FKBP1A Human)

Tags

myc

Expression

Mammalian

Available Since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only
FKBP-myc

Plasmid

#20211

Depositor

Carolyn Bertozzi

Article

Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

Insert

FKBP (FKBP1A Human)

Tags

myc

Expression

Mammalian

Available Since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only
pDGB3_alpha1-pAtUBQ10:Myc-GVG-HA-tE9-6xUAS-35Smini-HA-LRR-NEL-mcherry-Tnos

Plasmid

#211842

Purpose

Plant transient expression cassette for a Dexamethasone-inducible E3-DART ligase

Depositor

Marcela Rojas-Pierce

Article

Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

Inserts

LRR-NEL
GAL4-VP16-GR

Use

Synthetic Biology

Tags

HA, Myc, and mCherry

Expression

Plant

Promoter

6xUAS-35Smini and pUBQ10

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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pDGB3_alpha1-pAtUBQ10:Myc-GVG-HA-tE9-6xUAS-35Smini-HA-LRR-NEL-mcherry-Tnos

Plasmid

#211842

Purpose

Plant transient expression cassette for a Dexamethasone-inducible E3-DART ligase

Depositor

Marcela Rojas-Pierce

Article

Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

Inserts

LRR-NEL
GAL4-VP16-GR

Use

Synthetic Biology

Tags

HA, Myc, and mCherry

Expression

Plant

Promoter

6xUAS-35Smini and pUBQ10

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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pGL2-NOS2Promoter-Luciferase

Plasmid

#19296

Depositor

Charles Lowenstein

Article

Lowenstein et al Proc Natl Acad Sci U S A. 1993 Oct 15. 9

Insert

NOS2 Promoter (Nos2 Mouse)

Use

Luciferase

Expression

Mammalian

Available Since

Nov. 12, 2008

Availability

Academic Institutions and Nonprofits only

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pGL2-NOS2Promoter-Luciferase

Plasmid

#19296

Depositor

Charles Lowenstein

Article

Lowenstein et al Proc Natl Acad Sci U S A. 1993 Oct 15. 9

Insert

NOS2 Promoter (Nos2 Mouse)

Use

Luciferase

Expression

Mammalian

Available Since

Nov. 12, 2008

Availability

Academic Institutions and Nonprofits only

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