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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 8,392 results for: crispr grnas

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Showing: 7401 - 7420 of 8392 results

  1. pEvolvR-mCherry-SpRY-nCas9(D10A)-PolI5MΔ

    Plasmid
    #249074
    Purpose
    Expresses nucleus-localized SpRY-nCas9 (D10A) fused to PolI5MΔ and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.
    Depositor
    John Dueber, David Schaffer
    Article
    Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:
    Insert
    SpRY-nCas9-PolI5MΔ
    Use
    CRISPR
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    deletion of PolI5M N-terminal flap endonuclease d…
    Promoter
    CMV
    Available Since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249074-plasmid-map-sequence-id-496313

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  3. pXYB085

    Plasmid
    #239637
    Purpose
    Plant RNA Vision constructs with 164 bp gRNAs together with RFP input
    Depositor
    Xiaohan Yang
    Article
    Liu et al Plant Biotechnol J. 2025 May;23(5):1640-
    Inserts
    sfGFPn_Y66-P1_loop_01-stem01-gRNA09
    gRNA10-stem_02-P1_loop_03-internal guide sequence-Ribozyme-sfGFPn_Y66
    RFP
    Use
    Synthetic Biology
    Expression
    Plant
    Available Since
    June 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    239637-plasmid-map-sequence-id-477062

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  4. pXYB147

    Plasmid
    #239629
    Purpose
    Plant RNA Vision constructs with with 82 bp gRNAs targeting AT3G20840
    Depositor
    Xiaohan Yang
    Article
    Liu et al Plant Biotechnol J. 2025 May;23(5):1640-
    Inserts
    sfGFPn_Y66-P1_loop_01-stem01-gRNA05
    gRNA06-stem_02-P1_loop_03-internal guide sequence-Ribozyme-sfGFPn_Y66
    Use
    Synthetic Biology
    Expression
    Plant
    Available Since
    June 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    239629-plasmid-map-sequence-id-477058

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  5. pXYB084

    Plasmid
    #239636
    Purpose
    Plant RNA Vision constructs with 123 bp gRNAs together with RFP input
    Depositor
    Xiaohan Yang
    Article
    Liu et al Plant Biotechnol J. 2025 May;23(5):1640-
    Inserts
    sfGFPn_Y66-P1_loop_01-stem01-gRNA07
    gRNA08-stem_02-P1_loop_03-internal guide sequence-Ribozyme-sfGFPn_Y66
    RFP
    Use
    Synthetic Biology
    Expression
    Plant
    Available Since
    June 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    239636-plasmid-map-sequence-id-477052

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  6. pXYB086

    Plasmid
    #239638
    Purpose
    Plant RNA Vision constructs with 325 bp gRNAs together with RFP input
    Depositor
    Xiaohan Yang
    Article
    Liu et al Plant Biotechnol J. 2025 May;23(5):1640-
    Inserts
    sfGFPn_Y66-P1_loop_01-stem01-gRNA11
    gRNA12-stem_02-P1_loop_03-internal guide sequence-Ribozyme-sfGFPn_Y66
    RFP
    Use
    Synthetic Biology
    Expression
    Plant
    Available Since
    June 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    239638-plasmid-map-sequence-id-477050

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  13. pMOD_C2516

    Plasmid
    #91084
    Purpose
    Module C, Promoter: At7SL, Gene: Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol III
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)
    Use
    CRISPR
    Promoter
    At7SL
    Available Since
    July 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    91084-plasmid-map-sequence-id-182048

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  20. pFH51

    Plasmid
    #128213
    Purpose
    improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with OsU3 promoter module (pFH38)
    Depositor
    Vladimir Nekrasov
    Article
    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d
    Insert
    improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with OsU3 promoter module (pFH38)
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Available Since
    Aug. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    128213-plasmid-map-sequence-id-252899

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Showing: 7401 - 7420 of 8392 results