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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 13,612 results for: 11

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Showing: 7421 - 7440 of 13612 results

  7. MTK0_012

    Plasmid
    #123967
    Purpose
    Encodes the hROSA26 BxBI recognition site with Hygromycin resistance and mRuby2 landing pad with GFP dropout destination vector as a type 0 part to be used in the MTK system
    Depositor
    Hana El-Samad
    Publication
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    hRosa26-BxBI landing pad
    Expression
    Mammalian
    Available since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    123967-plasmid-map-sequence-id-251720

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  8. MTK3_009

    Plasmid
    #123724
    Purpose
    Encodes nuclear localized PHYB fused to activating domain of VP16 as Type 3 part to be used in the MTK system
    Depositor
    Hana El-Samad
    Publication
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PHYB-VP16-NLS
    Expression
    Mammalian
    Available since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    123724-plasmid-map-sequence-id-248657

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  9. PatoL-OG241

    Plasmid
    #72841
    Purpose
    Induced by acetoacetate to express GFP using atoSC two-component system in E. coli. Contains the atoDAEB promoter (Pato) with a strong RBS, Bba_B0034 (iGEM). OG241 backbone.
    Depositor
    Chris Barnes
    Publication
    Rutter et al Biotechnol Bioeng. 2021 Nov;118(11):4278
    Insert
    PatoL
    Expression
    Bacterial
    Promoter
    PatoL
    Available since
    Aug. 17, 2017
    Availability
    Academic Institutions and Nonprofits only
    72841-plasmid-map-sequence-id-133605

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  13. pAAV-EF1α1.1-FLEX-rc [ChrimsonR-GFP]

    Plasmid
    #108273
    Purpose
    AAV-mediated expression of ChrimsonR-GFP under the EF1α promoter (1.1kb short version), in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.
    Depositor
    Edward Boyden
    Publication
    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:
    Insert
    ChrimsonR-GFP
    Use
    AAV
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Chrimson K176R mutant
    Promoter
    EF1α promoter (1.1kb short version)
    Available since
    June 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    108273-plasmid-map-sequence-id-213551

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  14. pBbB8k-csg-nb112

    Plasmid
    #171702
    Purpose
    Arabinose-inducible csgBACEFG operon for curli fiber synthesis in which curli subunit CsgA has been fused to a nanobody targeting the spike protein of SARS-CoV-2 via a flexible linker.
    Depositor
    Neel Joshi
    Publication
    Birnbaum et al ACS ES T Water. 2022 Nov 11;2(11):1836-1
    Insert
    csgBACEFG, with nanobody fused to CsgA
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available since
    June 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    171702-plasmid-map-sequence-id-336472

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  15. pUC19-U6-MbCas12a crRNA-BsmBI cassette (BPK4449)

    Plasmid
    #114088
    Purpose
    U6 promoter crRNA entry vector used for all MbCas12a crRNAs (clone spacer oligos into BsmBI cassette)
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Publication
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    entry vector for MbCas12a crRNAs
    Expression
    Mammalian
    Available since
    Dec. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    114088-plasmid-map-sequence-id-220507

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  16. pCAG-hMbCas12a-NLS(nucleoplasmin)-3xHA (AAS2134)

    Plasmid
    #114090
    Purpose
    CAG promoter expression plasmid for human codon optimized MbCas12a nuclease with C-terminal NLS and HA tag
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Publication
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized MbCas12a
    Tags
    NLS(nucleoplasmin) and 3xHA
    Expression
    Mammalian
    Available since
    Dec. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    114090-plasmid-map-sequence-id-230476

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  17. JBEI-3716

    Plasmid
    #100169
    Purpose
    Used to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growth
    Depositor
    Jay Keasling
    Publication
    Anthony et al Metab Eng. 2009 Jan;11(1):13-9. doi: 10.
    Insert
    codon-optimized MK
    Expression
    Bacterial
    Available since
    Sept. 15, 2017
    Availability
    Academic Institutions and Nonprofits only
    100169-plasmid-map-sequence-id-193493

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  19. pCAG-RFP-p2a-postASAP

    Plasmid
    #178793
    Purpose
    Expresses voltage sensor targeted to dendrites and spines along with RFP
    Depositor
    Rafael Yuste
    Publication
    Cornejo et al Science. 2022 Jan 7;375(6576):82-86. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FingR.PSD95, ASAP2f and TagRFP
    Tags
    TagRFP
    Expression
    Mammalian
    Mutation
    mutations in amino acids L146G, S147T N149R, S150…
    Promoter
    CAG
    Available since
    April 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    178793-plasmid-map-sequence-id-359665

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Showing: 7421 - 7440 of 13612 results