We narrowed to 10,386 results for: Coli
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Plasmid#120799PurposeE. coli-C. difficile shuttle vector for xylose-inducible expression in C. difficile; Pxyl driving expression of red fluorescent protein (mCherryOpt)DepositorInsertPxyl::mCherryOpt
ExpressionBacterialMutation1.4 kb xylR-Pxyl DNA fragment from C. difficile R…PromoterPxylAvailable SinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSupAR-MbPylRS(DiZPK)
Plasmid#91705Purposeexpress Methanosarcina barkeri pyrrolysyl-tRNA synthetase/tRNA pair in E. coli to incorporate photocrosslinkers DiZPK, DiZSeK, or DiZHSeC into an in-frame amber codon site on a protein of interestDepositorInsertspylS
pylT
ExpressionBacterialMutationL274A, C313SAvailable SinceJuly 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHBintE
Plasmid#48136PurposeShuttle vector E. coli/B.meg., carrying a temperature sensitive origin of replication for Bacillus megaterium, selection marker erytromycine for B. megaterium, integration vectorDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceOct. 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSD117A
Plasmid#218141PurposeExpresses AviTag-6xHis-MBP-TEV-SD40 for protein purification from E. coliDepositorInsertSD40
TagsAviTag-6xHis-MBP-TEV-(GGGGS)2Available SinceJune 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-TS2126 RNA Ligase
Plasmid#119941PurposeExpress TS2126 RNA ligase in E. coliDepositorInsertTS2126 RNA ligase
TagsHis6ExpressionBacterialPromoterT7Available SinceMay 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHS1623
Plasmid#239833PurposeWT OrufIscB E. coli expression for recombinant purificationDepositorInsertOrufIscB
Tags14xHis-TwinStrep-bdSUMOExpressionBacterialPromoterT7Available SinceJuly 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGM202T7
Plasmid#69993PurposeE. coli/Streptomyces expression vector containing the T7-promoterDepositorInsertsaphII
tsr
rep
sso
T7 promoter
dso
His-Orf
TagsN-terminal and C-terminal His tag encoding sequen…ExpressionBacterialAvailable SinceNov. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAC-ZEAXipi
Plasmid#53287PurposeContains crtE, idi, crtI, crtY, crtB, and crtZ genes of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces zeaxanthin in E. coliDepositorInsertcrtE, idi, crtY, crtI, crtB, crtZ
UseLow copy number bacterial cloning vectorPromoterendogenous promotersAvailable SinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-PLS-KP6_rpoA-U
Plasmid#190967PurposeTest the U-to-C editing activity of KP6 on AtrpoA editing site, in E. coliDepositorInsertPLS-KP6
TagsHisExpressionBacterialPromoterT7Available SinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAC-CANTHipi
Plasmid#53301PurposeContains crtE, idi, crtI, crtY, and crtB genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and a crtO cDNA of Haematococcus pluvialis fused to a Trc promoter. Produces canthaxanthin in E. coli.DepositorInsertcrtO
UseLow copy number bacterial cloning vectorTags6HisPromoterTrcAvailable SinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBR322-TIMER
Plasmid#103056Purposemedium copy plasmid for expression of a variant of the fluorescent timer protein suitable for bacteria under the control of the constitutive ybaJp promoter (works in E. coli and Salmonella enterica)DepositorInsertTIMER-bac
ExpressionBacterialMutationmutations: S4T and S179TPromoterconstitutive ybaJp promoterAvailable SinceNov. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAC-BETA-At
Plasmid#53288PurposeContains crtE, crtB, and crtI genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and an lcyB cDNA of Arabidopsis thaliana. Use with pY2F to produce alpha-carotene in E. coli.DepositorAvailable SinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMA_Int
Plasmid#110096PurposeE. coli replicative vector which contains the mycophage integrase gene required for the attP/attB integration. Is co-transformed in trans together with pFLAG_attP, but cannot replicate in mycobacteria, and is thus lost shortly after electroporation.DepositorInsertmycophage integrase
UseRequired for transformation of attb integrating v…Available SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MH6-RspWYL1
Plasmid#108306PurposeExpresses E. coli codon optimized RspWYL1 in the pET28a backbone.DepositorInsertRspWYL1
UseCRISPRTagsmH6 tagExpressionBacterialPromoterlacAvailable SinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR_L1-YFP-Turbo-L2
Plasmid#127350Purposegateway entry vector for making N-terminal YFP-TurboID fusions with nuclear and non-nuclear proteinsDepositorInsertTurboID (BirA mutant)
UseGateway entry vectorTagsGS linker, V5, and YFPMutationQ65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…Promoterno promoterAvailable SinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET21a - NDH
Plasmid#89626PurposeExpresses recombinant 6HIS tagged T. cruzi NADP-dependent oxidoreductase in E. coli upon IPTG inductionDepositorInsertNAD(P)-dependent oxidoreductase
Tags6HIS and T7 tag (gene 10 leader)ExpressionBacterialAvailable SinceApril 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET21a - EtfA
Plasmid#89616PurposeExpresses recombinant 6HIS tagged T. cruzi electron transfer flavoprotein A in E. coli upon IPTG inductionDepositorInsertElectron transport flavoprotein alpha subunit
Tags6HIS and T7 tag (gene 10 leader)ExpressionBacterialAvailable SinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET21a - EtfB
Plasmid#89617PurposeExpresses recombinant 6HIS tagged T. cruzi electron transfer flavoprotein B in E. coli upon IPTG inductionDepositorInsertelectron-transfer-flavoprotein, alpha polypeptide
Tags6HIS and T7 tag (gene 10 leader)ExpressionBacterialAvailable SinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET21a - NFDD
Plasmid#89624PurposeExpresses recombinant 6HIS tagged T. cruzi NAD-FAD–dependent dehydrogenase in E. coli upon IPTG inductionDepositorInsertNAD/FAD dependent dehydrogenase
Tags6HIS and T7 tag (gene 10 leader)ExpressionBacterialAvailable SinceApril 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
Cut190*_pET21b
Plasmid#215404PurposeExpression construct for Cut190* gene, codon optimized for expression in E. coliDepositorInsertCut190*
ExpressionBacterialMutationS226P/R228SAvailable SinceMay 16, 2024AvailabilityAcademic Institutions and Nonprofits only