We narrowed to 36,683 results for: KAN

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pHcRed-NPM1c-C1

Plasmid

#131821

Purpose

Expresses HcRed-NPM1c (cytoplasmic mutant, human) in mammalian cells through transient transfection

Depositor

Mark Minden

Article

Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

Insert

NPM1 (NPM1 Human)

Tags

HcRed

Expression

Mammalian

Mutation

This is the cytoplasmic mutant causing AML, where…

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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hEGFR-mEGFP

Plasmid

#182878

Purpose

Mammalian expression of epidermal growth factor receptor (EGFR) fused to mEGFP

Depositor

Salvatore Chiantia

Article

Tzoneva et al Biomolecules. 2020 May 22;10(5). pii: bi

Insert

hEGFR-mEGFP (EGFR Human)

Expression

Mammalian

Available Since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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pETM33_EIF4E_eiF4E

Plasmid

#178474

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

EIF4E_eiF4E (EIF4E Human, Synthetic)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-G-Kras

Plasmid

#180571

Purpose

ddGFP sensor of Kras

Depositor

Won Do Heo

Article

Kim J et al Nat Commun. 2019 Jan 14;10(1):211. doi:

Insert

ddGFP sensor of Kras

Expression

Mammalian

Available Since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pEB2_mScarletI_mVenusNB-MrkH

Plasmid

#182291

Purpose

expresses c-di-GMP biosensor in E. coli

Depositor

Howard Berg

Article

Vrabioiu et al Proc Natl Acad Sci U S A. 2022 Feb 8;119

Insert

mVenusNB-MrkH

Tags

mVenusNB internal fusion to MrkH

Expression

Bacterial

Promoter

proC

Available Since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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pFX-mito-mKate2

Plasmid

#174178

Purpose

Express mKate2 with mitochondria-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

mKate2 with mitochondria-targeting sequence

Tags

the mitochondria-targeting sequence of the cytoch…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pFX-EGFP-Rab5

Plasmid

#174454

Purpose

Express EGFP with early endosome-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

EGFP with early endosome-targeting sequence

Tags

small GTPase (Rab5)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pFX-mCherry-Rab11

Plasmid

#174461

Purpose

Express mCherry with recycling endosome-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

mCherry with recycling endosome-targeting sequence

Tags

small GTPase (Rab11)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

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L1_lacZgRNA-Ck2

Plasmid

#136137

Purpose

L1 in position 2, to clone gRNA target using BbsI, lacZ blue-white screening

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-MpU6:lacZgRNA

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pmr101A-cPr-mCherry (KmR)

Plasmid

#177207

Purpose

Plasmid with p15A origin for constitutive mCherry expression in E. coli

Depositor

Alexey Merz

Insert

mCherry

Expression

Bacterial

Promoter

Synthetic constitutive promoter

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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PtxS1-c003

Plasmid

#173076

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

PtxS1

Expression

Bacterial

Mutation

D35-I221

Available Since

Nov. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pN1-QUE37C

Plasmid

#129318

Purpose

ATP measurement

Depositor

Yasushi Okada

Article

Yaginuma et al bioRxiv 2021.10.08.463131

Insert

QUEEN-37C

Expression

Mammalian

Available Since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pET41sIII_GST-ALC1

Plasmid

#173932

Purpose

Bacterial expression of ALC1

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

ALC1 (CHD1L Human)

Tags

GST

Expression

Bacterial

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pAT9651-BEAR-GFP

Plasmid

#162989

Purpose

BEAR target plasmid with split EGFP and disrupted 5' splice site

Depositor

Ervin Welker

Article

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

EGFP split with an intron between amino acids 95-96

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pME-PLIN2-tdTOMATO

Plasmid

#172533

Purpose

PLIN2-tdTOMATO fusion (zebrafish lipid droplet reporter) in middle entry vector for Gateway cloning

Depositor

Richard White

Article

Lumaquin et al Elife. 2021 Jun 11;10. pii: 64744. doi:

Insert

plin2-tdtomato

Mutation

S80R, A81S, E144D, I148F, and R415H mutations in …

Available Since

Sept. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221-SLCO1B1_STOP

Plasmid

#161354

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLCO1B1 (SLCO1B1 Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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p3E-TurboID

Plasmid

#166570

Purpose

Multisite Gateway 3' entry clone for fusions onto the N-terminus of TurboID. Parton lab clone JSS

Depositor

Rob Parton

Article

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

TurboID

Use

Gateway Cloning

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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mCherry-NES-SspB-MCS-P2A-iLIDcaax

Plasmid

#173869

Purpose

Mammalian expression of mCherry-NES-SspB-P2A-iLIDcaax (opto-control) containing multiple cloning sites (MCS)

Depositor

Takao Nakata

Article

Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

Insert

mCherry-NES-SspB-MCS-P2A-iLIDcaax

Tags

C-terminal CAAX-box and mCherry

Expression

Mammalian

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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EF1a-EGFP (no signal control)

Plasmid

#170167

Purpose

Mammalian Expression of no signal control

Depositor

Christopher Moxham

Type

Empty backbone

Expression

Mammalian

Mutation

n/a

Available Since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pSF279

Plasmid

#167674

Purpose

Intermediate vector for five gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

attL1-ccdB-attL2

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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