We narrowed to 25,208 results for: promoter
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Plasmid#82701PurposeAAV backbone with a minimal H1/TO promoter driving expression of a sgRNA that targets exon 3 of the mouse Tet2 locus. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.DepositorInsertH1/TO gRNA Expression Cassette Containing Tet2 gRNA
UseAAV and CRISPRTagsCMV promoter driving expression of TetR-P2A-GFP-K…ExpressionMammalianAvailable SinceDec. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
FLAG-SFPQY527A
Plasmid#166961PurposeExpresses FLAG-tagged SFPQ Y527A protein in pcDNA3.1DepositorAvailable SinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
PLL5.0-Anillin ShRNA-GFP-AnillinFL
Plasmid#187272PurposeLentiviral expression of shRNA targeting Anillin + reconstitution of Anillin full lengthDepositorInsertAnillin ,hsAnillin, Entrez Gene ANLN (a.k.a. FSGS8, Scraps, scra) (ANLN Human)
UseLentiviralTagsEGFPPromoterU6, CMVAvailable SinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pROF441
Plasmid#155336PurposeBinary vector for expression of a gRNA targeting AtAP1 promoterDepositorInsertLB_PAtU6-26-gRNA(PAtAP1)-sgRNA_RB
UseCRISPR and Synthetic BiologyExpressionPlantAvailable SinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
GST-PS-Sos1 (PRM) - Cys (XSB364)
Plasmid#78506PurposeConstruct for expressing Sos1 proline-rich motif in E. Coli, with a C-terminal Cys for labelingDepositorInsertSos1 -Cys (PRM) (SOS1 Human)
TagsGST followed by a prescission protease claevage s…ExpressionBacterialAvailable SinceJune 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMT-myl7-cytoBirA-2A-mCherry_Ras
Plasmid#80060PurposeMyl7 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
UseZebrafish biotaggingTags3x HAExpressionBacterialPromoterMyl7 promoterAvailable SinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSLPB2B-SMASh-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin
Plasmid#187949PurposeSMASh degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.DepositorInsertSMASh-SpdCas9-tagRFPt-P2A-tagBFP
UseCRISPR and Synthetic BiologyTagsGGS linkerExpressionMammalianAvailable SinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDB.His.MBP.3C CARD8 UPA-CARD WT
Plasmid#164021PurposeBacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 CARDDepositorAvailable SinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
LZRS ERTm RasN17
Plasmid#21198DepositorAvailable SinceJune 16, 2010AvailabilityAcademic Institutions and Nonprofits only -
7432 Bicistronic_GFP_ires_puro
Plasmid#64336PurposeThis a retroviral expression plasmid expressing GFP along with puro resistance geneDepositorInsertGFP
UseRetroviralTagsGFPAvailable SinceJuly 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLX307 ARHGAP30
Plasmid#117738PurposeOpen reading frame vector encoding ARHGAP30DepositorAvailable SinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-LAP-KNL1-M3
Plasmid#115896PurposeUsed for expression of siRNA-resistant KNL1-M3 (modified codons 258 and 259) from the FRT site of FlpIn cells upon addition of doxycyclin.DepositorInsertKNL1-M3 (KNL1 Human)
TagsEYFPExpressionMammalianMutationFragment of KNL1 containing three KNL1 repeat seq…Available SinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLX307 DEDD
Plasmid#117744PurposeOpen reading frame vector encoding DEDDDepositorAvailable SinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPKm-230
Plasmid#90499PurposepSIN - EF-1alpha - PIF3 - MTAD - IRES - PhyB - GBD, dual vector of PIF3-MTAD under EF-1 alpha promoter and PhyB-DBD under IRES promoter; See growth conditions below.DepositorInsertmito-tFD and mito-tFNR
UseLentiviralAvailable SinceJune 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGL3-IRES-Lin28b-P3
Plasmid#64794PurposeLuciferase expression driven by Lin28b promoter P3DepositorInsertLin28b promoter P3 (LIN28B Human)
UseLuciferaseAvailable SinceJuly 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 myc-mβTrCP WD1-7 (241-569)
Plasmid#62977PurposeExpresses myc-tagged mouse βTrCP residues 241-569DepositorAvailable SinceMay 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGL-p21UTRm1&2
Plasmid#20880DepositorInsertp21 3'UTR site 1 and 2 mutation (Cdkn1a Mouse)
UseLuciferaseExpressionMammalianMutationPredicted miRNA binding site 1 (Position ~420) GC…Available SinceAug. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pNCST-AausFP3
Plasmid#129501PurposeExpresses AausFP3 constitutively in E. coli (most strains)DepositorInsertAausFP3
ExpressionBacterialPromotersynthetic constitutive (stationary phase) promote…Available SinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-VprBP (C590)
Plasmid#133589PurposeExpresses human VprBP C590 mutant in mammalian cellsDepositorInsertVpr (HIV-1) binding protein (DCAF1 Human)
TagsHAExpressionMammalianMutationC590 mutation of human VprBP lacking the N-termin…Available SinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
Coexpressed Free GFP-NES
Plasmid#182491PurposeYeast integrative plasmid for expressing ERG20 (GAL10 promoter) and fusion protein GFP-AcNES1 (GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertsFPPS
GFP-NES
UseSynthetic Biology; Metabolic engineeringExpressionYeastPromoterGAL10 and GAL7Available SinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only