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pAV0756

Plasmid

#133508

Purpose

Expression of 3mCherry tagged CRIB domain to monitor active Cdc42

Depositor

Sophie Martin

Publication

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pHis5(StuI)-p(act1)-CRIB(gic2aa2-181)-3mCherry-bsdMX

Expression

Yeast

Available since

Nov. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pBsaGSCYP101A1

Plasmid

#108206

Purpose

CYP101A1

Depositor

Teruyuki Nagamune

Publication

Iwata et al Biotechnol J. 2018 Nov;13(11):e1700662.

Insert

CYP101A1

Expression

Bacterial

Promoter

lac promoter

Available since

April 15, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR221-WNT3A

Plasmid

#79501

Purpose

MultiSite Gateway entry clones, second fragment (attL5, attL2) Human WNT3A CDS

Depositor

Peter Jon Nelson

Publication

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

WNT3A (WNT3A Human)

Use

Gateway Cloning

Promoter

no Promoter

Available since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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mEos2-CAF1-C-10

Plasmid

#57351

Purpose

Localization: Chromatin Assembly Factor 1, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Publication

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

CAF1

Tags

mEos2

Expression

Mammalian

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221-Phelo

Plasmid

#48995

Purpose

Gateway adapted transfer of Phleomycin resistance gene

Depositor

Rick Tarleton

Publication

Xu et al BMC Microbiol. 2009 May 11;9:90. doi: 10

Insert

Sh ble

Use

Gateway attb1 & attb2

Available since

April 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pfastbacHTb/mClock26-384

Plasmid

#47336

Purpose

mClock fragment cloned into pfastbacHTb vector with Hexa His tag

Depositor

Joseph Takahashi

Publication

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

Clock (Clock Mouse)

Tags

His and Tev

Mutation

aa 26-384

Promoter

PH

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
pVRb15_up436

Plasmid

#49709

Depositor

Christopher Voigt

Publication

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

sfgfp

Use

Synthetic Biology

Expression

Bacterial

Promoter

Pecf15_up436

Available since

Jan. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pVRb03_up1198

Plasmid

#49706

Depositor

Christopher Voigt

Publication

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

sfgfp

Use

Synthetic Biology

Expression

Bacterial

Promoter

Pecf03_up1198

Available since

Jan. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pFAB3725

Plasmid

#47837

Purpose

BIOFAB RFP reporter plasmid for measuring promoter 6 + BCD1 efficiency.

Depositor

Drew Endy

Publication

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

promoter 6 and BCD1

Use

Synthetic Biology

Expression

Bacterial

Promoter

see insert

Available since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pFAB3833

Plasmid

#47839

Purpose

BIOFAB RFP reporter plasmid for measuring promoter 8 + BCD1 efficiency.

Depositor

Drew Endy

Publication

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

promoter 8 and BCD1

Use

Synthetic Biology

Expression

Bacterial

Promoter

see insert

Available since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pfastbac/mBmal62-447

Plasmid

#47335

Purpose

mBmal fragment cloned into pfastbac and crystallized

Depositor

Joseph Takahashi

Publication

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

BMAL (Bmal1 Mouse)

Mutation

aa 62-447

Available since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
V32V

Plasmid

#29561

Depositor

Steven Vogel

Publication

Thaler et al Proc Natl Acad Sci U S A. 2009 Apr 14. 1

Insert

Venus-32-Venus

Use

Anisotropy and brightness standard

Expression

Mammalian

Available since

June 23, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
MSCV-PM mir shHoxB9 b

Plasmid

#27020

Depositor

Joan Massague

Publication

Nguyen et al Cell. 2009 Jul 10. 138(1):51-62.

Insert

mir HoxB9 3b

Use

RNAi and Retroviral

Expression

Mammalian

Available since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only
FepA

Plasmid

#26039

Depositor

Johann Deisenhofer

Publication

Buchanan et al Nat Struct Biol. 1999 Jan . 6(1):56-63.

Insert

FepA (fepA E Coli)

Expression

Bacterial

Available since

Dec. 3, 2010

Availability

Academic Institutions and Nonprofits only
pSKB3.TM1777

Plasmid

#11421

Depositor

Sung-Hou Kim

Publication

Shin et al Biochemistry. 2003 Nov 25. 42(46):13429-

Insert

NusA (nusA T. maritima)

Tags

6x His and TEV protease site

Expression

Bacterial

Available since

April 10, 2006

Availability

Academic Institutions and Nonprofits only
pIR35-WP_073484372.1

Plasmid

#232707

Purpose

Expresses the imine reductase WP_073484372.1 (IR35)

Depositor

Simon d'Oelsnitz, Pamela Silver

Publication

d'Oelsnitz et al Nat Chem Biol. 2026 Jul 23. doi: 10.1038

Insert

WP_073484372.1

Use

Synthetic Biology

Expression

Bacterial

Available since

July 2, 2026

Availability

Academic Institutions and Nonprofits only

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nTurboID-HA_pEarleyGate100

Plasmid

#247582

Purpose

A Gateway-compatible vector harboring a nTurboID–HA fusion and conferring BASTA resistance in plants

Depositor

Juan Dong

Publication

Huang et al Proc Natl Acad Sci U S A. 2025 Aug 12;12

Insert

nTurboID

Tags

HA

Expression

Plant

Available since

Jan. 6, 2026

Availability

Academic Institutions and Nonprofits only

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pDIRECT_26K

Plasmid

#91152

Purpose

Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:TALEN, Plant Selection: PvUbi2:bar

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Engineering Reagent: ZmUbi:TALEN

Use

TALEN

Expression

Plant

Available since

Jan. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pDIRECT_23J

Plasmid

#91142

Purpose

Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:TALEN, Plant Selection: 2x35S:bar

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Engineering Reagent: 35S:TALEN

Use

TALEN

Expression

Plant

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pYPQAT02

Plasmid

#223374

Purpose

T-DNA vector for SpCas9 mediated mutagenesis for plants; NGG PAM; Cas9 was driven by ZmUbi1 and the sgRNA was driven by AtU3 promoter; BASTA for plants selection.

Depositor

Yiping Qi

Publication

Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

Insert

ZmUbi-SpCas9-AtU3-gRNA scaffold

Use

CRISPR

Tags

3x FLAG and NLS

Expression

Plant

Available since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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