We narrowed to 17,080 results for: Por

1. pGL3 T2P 2.6

   Plasmid

   #131151

   Purpose

   To investigate activity of a 2.6 kb region upstream of mouse Trib2.

   Depositor

   Karen Keeshan

   Article

   Rishi et al Blood. 2014 Apr 10;123(15):2389-400. doi

   Insert

   -2.6kb - 0 of mTrib2 upstream of gene transcriptional start site (Trib2 Mouse)
   

   Use

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGL3 T2P 500

   Plasmid

   #131150

   Purpose

   To investigate activity of a 500 bp region upstream of mouse Trib2.

   Depositor

   Karen Keeshan

   Article

   Rishi et al Blood. 2014 Apr 10;123(15):2389-400. doi

   Insert

   -500 - 0 of mTrib2 upstream of gene transcriptional start site (Trib2 Mouse)
   

   Use

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. A3H HapII R175/6E-Cas9n-UGI

   Plasmid

   #119142

   Purpose

   Base editor made from APOBEC3H Haplotype II RNA binding mutant RR175/6EE

   Depositor

   Reuben Harris

   Article

   Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

   Insert

   APOBEC3H Haplotype II RR175/6EE-Cas9 nickase-UGI
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   A3H Hap II RR175/6EE

   Promoter

   CMV

   Available Since

   June 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. N229A Lo-MetQ

   Plasmid

   #118268

   Purpose

   N229A Mutation in Methionine Binding Protein (MetQ) used to solve crystal structure

   Depositor

   Douglas Rees

   Article

   Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p

   Insert

   MetQ (N229A) (metQ E. coli)
   

   Use

   Tags

   10x His Tag and Enterokinase Cleavage Site

   Expression

   Bacterial

   Mutation

   Changed Asparagine (Asn) 229 to Alanine (Ala)

   Promoter

   T7 Promoter

   Available Since

   Jan. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA-h-mmPtch1-B-V111FL114FI766F-HA-TagBFP

   Plasmid

   #120911

   Purpose

   Expresses BFP tagged Ptch1-B (V111FL114FI766F)

   Depositor

   Philip Beachy

   Article

   Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

   Insert

   Ptch1 (Ptch1 Mouse)
   

   Use

   Tags

   HA and TagBFP

   Expression

   Mammalian

   Mutation

   deleted amino acids 620-643, truncated at 1291, Y…

   Promoter

   CMV

   Available Since

   Jan. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA-h-mmPtch1-B-V111FL114FW115A-HA-GFP

   Plasmid

   #120908

   Purpose

   Expresses Ptch1-B (V111FL114FW115A)

   Depositor

   Philip Beachy

   Article

   Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

   Insert

   Ptch1 (Ptch1 Mouse)
   

   Use

   Tags

   GFP and HA

   Expression

   Mammalian

   Mutation

   deleted amino acids 620-643, truncated at 1291

   Promoter

   CMV

   Available Since

   Jan. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA-h-mmPtch1-B-I766FV111F-HA-GFP

   Plasmid

   #120907

   Purpose

   Expresses Ptch1-B (I766FV111F)

   Depositor

   Philip Beachy

   Article

   Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

   Insert

   Ptch1 (Ptch1 Mouse)
   

   Use

   Tags

   GFP and HA

   Expression

   Mammalian

   Mutation

   deleted amino acids 620-643, truncated at 1291

   Promoter

   CMV

   Available Since

   Jan. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA-h-mmPtch1-B-Y233C-HA

   Plasmid

   #120904

   Purpose

   Expresses Ptch1-B (Y233C) for function assays

   Depositor

   Philip Beachy

   Article

   Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

   Insert

   Ptch1 (Ptch1 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   deleted amino acids 620-643, truncated at 1291, Y…

   Promoter

   CMV

   Available Since

   Jan. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA-h-mmPtch1-B-L234C-HA

   Plasmid

   #120905

   Purpose

   Expresses Ptch1-B (L234C)

   Depositor

   Philip Beachy

   Article

   Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

   Insert

   Ptch1 (Ptch1 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   deleted amino acids 620-643, truncated at 1291, L…

   Promoter

   CMV

   Available Since

   Jan. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET22b-ecDHFR-D27S/Y100F

    Plasmid

    #110827

    Purpose

    Bacterial expression of E.coli DHFR D27S/Y100F mutant

    Depositor

    Stephen Benkovic

    Article

    Liu et al Proc Natl Acad Sci U S A. 2014 Dec 23;11

    Insert

    ecDHFR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D27 mutated to S27, Y100 mutated to F100

    Promoter

    T7

    Available Since

    June 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA5/FRT/TO-TPI_PTC160-xrRNA-4H

    Plasmid

    #108378

    Purpose

    FRT integration of TPI reporter with premature termination codon (PTC160); enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)

    Depositor

    Niels Gehring

    Article

    Boehm et al Nat Commun. 2016 Dec 5;7:13691. doi: 10.

    Insert

    PTC160 TPI reporter with MVE xrRNA and 4H probe binding sites (TPI1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA5/FRT/TO-TPI_WT-xrRNA-4H

    Plasmid

    #108377

    Purpose

    FRT integration of wild type TPI reporter; enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)

    Depositor

    Niels Gehring

    Article

    Boehm et al Nat Commun. 2016 Dec 5;7:13691. doi: 10.

    Insert

    Wild type TPI reporter with MVE xrRNA and 4H probe binding sites (TPI1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHW564 (Pmyo-2::cGAL-N(C1A)::let-858 3'UTR)

    Plasmid

    #107739

    Purpose

    mutated cGAL-N (C1A) driver under the control of C. elegans myo-2 promoter

    Depositor

    Paul Sternberg

    Article

    Wang et al Proc Natl Acad Sci U S A. 2018 Mar 26. p

    Insert

    NLS::cGAL(DBD)::gp41-1-N-intein(C1A)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    change the first cysteine of gp41-1 N-intein to a…

    Promoter

    C. elegans myo-2 promoter

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pX458-sgRNA_Ago1_4

    Plasmid

    #73536

    Purpose

    Expresses SpCas9, GFP, and sgRNA targeting Ago1

    Depositor

    Constance Ciaudo

    Article

    Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

    Insert

    AGO1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    Feb. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

15. pPN018

    Plasmid

    #91664

    Purpose

    Express sgRNA targeting human SCN2A

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    SCN2A (SCN2A Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available Since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pPN282

    Plasmid

    #91665

    Purpose

    Express sgRNA targeting human SDCCAG8

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    SDCCAG8 (SDCCAG8 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available Since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pPN283

    Plasmid

    #91666

    Purpose

    Express sgRNA targeting human SDCCAG8

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    SDCCAG8 (SDCCAG8 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available Since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pPN272

    Plasmid

    #91643

    Purpose

    Express sgRNA targeting human MMP16

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    MMP16 (MMP16 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available Since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pPN273

    Plasmid

    #91644

    Purpose

    Express sgRNA targeting human MMP16

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    MMP16 (MMP16 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available Since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pPN278

    Plasmid

    #91653

    Purpose

    Express sgRNA targeting human PTN

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    PTN (PTN Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available Since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge