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pDONR223_TNFRSF17_WT

Plasmid

#82263

Purpose

Gateway Donor vector containing TNFRSF17 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Publication

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

TNFRSF17 (TNFRSF17 Human)

Use

Gateway Cloning

Mutation

N81S; K86E

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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mKate2-P2A-APEX2-OSBP

Plasmid

#67664

Purpose

Mammalian expression vector driving APEX2-OSBP (Human OSBP domain, [marks PI4P] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.

Depositor

Rob Parton

Publication

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

mKate2-P2A-APEX2-OSBP

Expression

Mammalian

Promoter

CMV

Available since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pDONR223-CDK5

Plasmid

#23699

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

CDK5 (CDK5 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pDONR223-ACVR2A

Plasmid

#23654

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

ACVR2A (ACVR2A Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pH_35S/mEGFP

Plasmid

#135321

Purpose

mEGFP expression regulated by the CaMV35S promoter, co-expressed hygromycin phosphotransferase selectable marker (hpt)

Depositor

Mathias Pribil

Publication

Behrendorff et al Physiol Plant. 2020 Feb 25. doi: 10.1111

Insert

mEGFP

Expression

Plant

Promoter

Cauliflower mosaic virus 35S

Available since

Jan. 6, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDONR223-PRKCZ

Plasmid

#23724

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PRKCZ (PRKCZ Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pCD5-PR8-TEV-sfGFP-twinStrep

Plasmid

#157933

Purpose

Expresses influenza A virus trimeric hemagglutinin fused to a sfGFP

Depositor

Robert de Vries

Publication

Nemanichvili et al J Mol Biol. 2019 Feb 15;431(4):842-856.

Insert

A/PuertoRico/8/34 hemagglutinin (HA Synthetic)

Tags

sfGFP & TwinStrep

Expression

Mammalian

Mutation

E190D

Promoter

CMV

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pAC-EPSILON

Plasmid

#53276

Purpose

Contains ctrE, crtB, and crtI genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and the lcyE gene of Lactuca sativa and thereby produces epsilon-carotene in Escherichia coli

Depositor

Francis X Cunningham Jr

Publication

Cunningham FX et al Plant J. 2005 Feb;41(3):478-92.

Insert

lcyE

Use

Low copy number bacterial cloning vector

Promoter

lac

Available since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDONR223-RIOK1

Plasmid

#23759

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

RIOK1 (RIOK1 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-PIP5K2C

Plasmid

#23450

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PIP5K2C (PIP4K2C Human)

Use

Gateway Cloning

Mutation

1MM

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pAG414GAL-ccdB-HA

Plasmid

#14239

Depositor

Susan Lindquist

Publication

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway Cloning

Tags

3HA

Expression

Yeast

Available since

March 29, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDN-Cherry-sgRNA

Plasmid

#221387

Purpose

episomal (HygR), with constitutive mCherry (Psmyc) and AHT-inducible sgRNA cloning site, including Golden Gate for multiple sgRNAs

Depositor

Deborah Hung

Publication

Neo et al J Bacteriol. 2024 Mar 21;206(3):e0033523

Insert

none, cloning vector for introduction of targeting sequences

Use

CRISPR

Available since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223-DAPK3

Plasmid

#23436

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

DAPK3 (DAPK3 Human)

Use

Gateway Cloning

Mutation

1MM

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pDONR223-PLXNA3

Plasmid

#23518

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PLXNA3 (PLXNA3 Human)

Use

Gateway Cloning

Mutation

L1655M

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDEST27-Vinculin

Plasmid

#20144

Depositor

Valeri Vasioukhin

Publication

Lien et al J Cell Biol. 2008 Dec 15. 183(6):989-97.

Insert

pDEST27-Vinculin (Vcl Mouse)

Expression

Mammalian

Available since

March 31, 2009

Availability

Academic Institutions and Nonprofits only
pKRaION1-N1

Plasmid

#177801

Purpose

Mammalian expression of green fluorescent potassium indicator KRaION1

Depositor

Edward Boyden

Publication

Torres Cabán et al ACS Sens. 2022 May 27;7(5):1336-1346. do

Insert

KRaION1

Expression

Mammalian

Promoter

CMV

Available since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pAG416GPD-EGFP-ccdB

Plasmid

#14316

Depositor

Susan Lindquist

Publication

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway Cloning

Tags

EGFP

Expression

Yeast

Available since

June 18, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pAG423GPD-EGFP-ccdB

Plasmid

#14318

Depositor

Susan Lindquist

Publication

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway Cloning

Tags

EGFP

Expression

Yeast

Available since

June 18, 2007

Availability

Academic Institutions and Nonprofits only

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pAG415GPD-ccdB-DsRed

Plasmid

#14362

Depositor

Susan Lindquist

Publication

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway Cloning

Tags

DsRed

Expression

Yeast

Available since

June 18, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAG306GAL-ccdB-EGFP

Plasmid

#14187

Depositor

Susan Lindquist

Publication

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway Cloning

Tags

EGFP

Expression

Yeast

Available since

March 16, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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